The 293FT Cell line is ideal for generating high-titer lentivirus using the ViraPower™ Lentiviral Expression System. The 293FT Cell Line is a fast-growing, highly transfectable clonal isolate derived from human embryonal kidney cells transformed with the SV40 large T antigen. When a ViraPower™ expression vector and the ViraPower™ Lentiviral Packaging Mix are co-transfected into 293FT cells, high levels of the viral RNA and the gag⁄pol and rev proteins required for packaging are produced.
Advantages • Fast growing • Highly transfectable • Produces high viral titers • Allows very high levels of protein
Key Features • Presence of the SV40 large T antigen allows very high levels of protein to be expressed from vectors containing the SV40 origin. Higher, in fact, than other 293 cells or the T antigen-transformed COS cells classically used.
Kit includes • 293FT Cell Line provided as a frozen stock in 1 ml of 90% complete media and 10% DMSO