293FT Cell Line

본 제품은 액체 질소로 배송 및 보관되는 세포 관련 제품으로, 해당 제품 구매 시 세포 사용 가이드라인을 반드시 확인해주십시오. 세포사용가이드라인 다운로드

본 제품은 LMO 제품으로, 고객 분께서 LMO 신고 시스템을 통해 직접 수입 신고를 진행해주셔야 합니다. 자세히보기

인용 및 참조 문헌 (3)

Invitrogen™

293FT Cell Line

293FT Cell line은 ViraPower™ Lentiviral Expression System을 사용해 고역가 lentivirus를 생성하는 데 이상적입니다. 293FT Cell Line은 인간 태아 신장세포에서 유래된자세히 알아보기

카탈로그 번호	수량
R70007	1 x 10⁷ cells

카탈로그 번호 R70007

제품 가격(KRW)

999,000

온라인 행사

Ends: 31-Dec-2025

1,110,000

할인액 111,000 (10%)

카트에 추가하기

1 x 10⁷ cells

제품 가격(KRW)

999,000

온라인 행사

Ends: 31-Dec-2025

1,110,000

할인액 111,000 (10%)

카트에 추가하기

293FT Cell line은 ViraPower™ Lentiviral Expression System을 사용해 고역가 lentivirus를 생성하는 데 이상적입니다. 293FT Cell Line은 인간 태아 신장세포에서 유래된 성장 속도가 빠르고 transfection 효율이 높은 클론 부분으로 SV40 large T antigen으로 transformation됩니다. ViraPower™ expression vector와 ViraPower™ Lentiviral Packaging Mix를 293FT 세포에 co-transfection하면 viral RNA와 gag⁄pol, rev protein (포장에 필요)이 생성됩니다.

장점
• 빠른 성장
• 높은 transfection 효율
• 높은 바이러스 역가
• 매우 높은 수준의 단백질

주요 특징
• SV40 large T antigen이 있어 SV40 기원 물질을 함유한 벡터에서 단백질이 매우 높게 발현되게 합니다. 실제로 기존에 이용된 다른 293 cells 또는 T antigen-transformed COS cell 보다 발현률이 높습니다.

Kit 구성
• 293FT Cell Line은 1 ml 동결 제품으로 제공됩니다(90% complete media+10% DMSO).

본 제품은 연구용으로만 사용가능합니다. 치료 또는 진단 목적으로 사용할 수 없습니다.

For research use only. Not intended for any therapeutic or diagnostic use.

미디어 권장 사항SFM (Serum Free Medium)

셀 수1 x 10⁷

제품라인ViraPower

수량1 x 10⁷ cells

세포주293FT

종Human

Unit SizeEach

구성 및 보관

• 293FT Cell Line (Store in liquid nitrogen)

자주 묻는 질문(FAQ)

Do 293FT cells lift off the plate during lentivirus production?

If 293FT cells detach shortly after transfection (4 hours to overnight):

- This may be a sign of Lipofectamine 2000 toxicity. Cells may have been plated too sparsely prior to transfection.
- The cells may not have been handled gently enough (these cells have a tendency to lift off easily).
- The cells may have been kept at room temperature for too long.

If cells detach 48 to 72 hours post-transfection:
- If the cells lift off in large sheets, this may be a sign of lentivirus production.

What are syncytia?

Syncytia are large multi-nucleated cells that result from VSV-G-induced fusion with neighboring 293FT producer cells. Syncytia production is indicative of high transfection efficiency and lentivirus production. Keep in mind, though, that the absence of syncytia does not mean that virus will not be produced.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

Why do I have to culture 293FT cells under Geneticin antibiotic selection?

The 293FT cell line stably expresses the SV40 large T antigen from the pCMVSPORT6Tag.neo plasmid that contains the neomycin resistance marker. In order to maintain the plasmid/phenotype, the cells have to be routinely cultured in medium containing Geneticin (G418) antibiotic at a concentration of 500 µg/mL.

Do you recommend a specific FBS for culturing 293FT cells? Which plastic plates do you recommend?

We use Mycoplasma-tested Gibco FBS (Cat. No. 16000-044). We have observed that when 293FT cells are cultured in the presence of this FBS following the instructions in the manual, virus production is better than that obtained with many other serum sources. We use the following plasticware for 293FT cells:

T175-Fisher Cat. No. 10-126-13; this is a Falcon flask with 0.2 µm vented plug seal cap.
T75-Fisher Cat. No. 07-200-68; this is a Costar flask with 0.2 µm vented seal cap.
100 mm plate-Fisher Cat. No. 08-772E; this is a Falcon tissue culture-treated polystyrene plate.
We get excellent adherence on these plates under routine cell culture/maintenance conditions.

What are the morphological changes that I should be looking for in my 293FT cells as signs of lentivirus production?

During lentivirus production, 293FT cells undergo the following morphological changes:
- They become multi-nucleated (syncytia development)
- They start to look like balloons or as if they are about to explode
- They often, but not always, lift off from the surface
- Untransfected 293FT cells leave empty spaces and “pile” up at other spots in the flask

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all 293FT Cell Line FAQs

인용 및 참조 문헌 (3)

인용 및 참조 문헌

Abstract

The interaction between HIV-1 Gag and human lysyl-tRNA synthetase during viral assembly.

Authors:Javanbakht H, Halwani R, Cen S, Saadatmand J, Musier-Forsyth K, Gottlinger H, Kleiman L,

Journal:J Biol Chem

PubMed ID:12756246

'Human lysyl-tRNA synthetase (LysRS) is a tRNA-binding protein that is selectively packaged into HIV-1 along with its cognate tRNALys isoacceptors. Evidence exists that Gag alone is sufficient for the incorporation of LysRS into virions. Herein, using both in vitro and in vivo methods, we begin to map regions in Gag ... More

RIP140-targeted repression of gene expression in adipocytes.

Authors:Christian M, Kiskinis E, Debevec D, Leonardsson G, White R, Parker MG,

Journal:Mol Cell Biol

PubMed ID:16227589

'Ligand-dependent repression of nuclear receptor activity forms a novel mechanism for regulating gene expression. To investigate the intrinsic role of the corepressor RIP140, we have monitored gene expression profiles in cells that express or lack the RIP140 gene and that can be induced to undergo adipogenesis in vitro. In contrast ... More

A novel method for long term bone marrow culture and genetic modification of murine neutrophils via retroviral transduction.

Authors:Zemans RL, Briones N, Young SK, Malcolm KC, Refaeli Y, Downey GP, Worthen GS,

Journal:J Immunol Methods

PubMed ID:19010330

'Neutrophils are a critical component of the innate immune response to invading microbial pathogens. However, an excessive and/or prolonged neutrophil response can result in tissue injury that is thought to underlie the pathogenesis of various inflammatory diseases. The development of novel therapeutic strategies for inflammatory diseases depends on an improved ... More