T-REx™-HeLa Cell Line

Catalog number: R71407

Invitrogen™  Related applications: Protein Expression

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Description

T-REx™ Cell Lines stably express the tetracycline repressor protein (Table 1). They save significant time and effort when using the T-REx™ System. The T-REx™ Cell Lines are functionally tested by transient transfection with the positive control vector pcDNA™4⁄TO⁄lacZ. T-REx™ Cell Lines exhibit extremely low basal expression levels in the repressed state and high expression upon induction with tetracycline (Figure 1).
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

PCR Method: 1-Step qRT-PCR
Purity or Quality Grade: UP (Ultra Pure)
Reverse Transcriptase: ArrayScript™ UP
Polymerase: AmpliTaq Gold® DNA Polymerase
Detection Method: Primer-Probe Detection
Primer-Probe Compatibility: TaqMan®
Reaction Speed: Standard
For Use With (Equipment): 7300 System, 7700 System, 7500 System, StepOnePlus™, Standard Mode, StepOne™, Standard Mode, 7900HT System, 7000 System
Sample Type (General): RNA
Passive Reference Dye: ROX (Pre-mixed)
Product Size: 200 reactions
Shipping Condition: Wet Ice

Contents & storage

3 x 106 cells are supplied frozen in 1 ml 90% complete medium and 10% DMSO. Store in liquid nitrogen. Cells are guaranteed stable for 6 months when properly stored.

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