Human adipose-derived stem cells (ADSCs) are isolated from human lipoaspirate tissue and cryopreserved from primary cultures. ADSCs have phenotypic and functional characteristics very similar to those of bone marrow-derived mesenchymal stem cells. Before cryopreservation, the ADSCs are expanded for one passage in MesenPRO RS™ Medium, a reduced-serum (2%) medium that improves MSC expansion compared to conventional serum supplemented media. The StemPro® Human Adipose-Derived Stem Cell Kit contains cryopreserved normal human ADSCs and MesenPRO RS™ Medium - together, these components ensure optimal ADSC growth and expansion. Each lot of ADSCs originates from a single donor of human lipoaspirate tissue. Each vial of ADSCs contains cells that can differentiate into multiple mature cell phenotypes in vitro, including adipocytes, osteoblasts, and chondrocytes. ADSCs can be expanded to 4-5 passages before they lose their ability to grow or differentiate into all potential phenotypes. The ADSCs express the following flow cytometry cell-surface protein profile: positive CD29, CD44, CD73, CD90, CD105, CD166; negative CD14, CD31, CD45, Lin1.
Applications: Formulated for the growth and expansion of mesenchymal stem cells.
Note: MesenPRO RS™ Medium (Cat. no. 12746-012), composed of MesenPRO RS™ Growth Supplement and MesenPRO RS™ Basal Medium, is available for purchase separately.
For Research Use Only. Not for use in diagnostic procedures.
Stably Integrated Gene or Element:
Stem Cells (Mesenchymal)
Contents & storage
This kit is shipped in two parts with separate storage requirements. These components are not sold individually:
–StemPro® Human Adipose-Derived Stem Cells (one vial containing ~1 x 106 cells; store in liquid nitrogen) and MesenPRO RS™ Growth Supplement (10 ml; store in the dark at -5 to -20°C) –MesenPRO RS™ Basal Medium (500 ml; store in the dark at 2-8°C)