Flp-In™ 293 T-REx cell lines is designed for rapid generation of stable cell lines that ensure homogenous expression of your protein of interest from a Flp-In™ expression vector. These cells contain a single stably integrated FRT site at a transcriptionally active genomic locus. Targeted integration of a Flp-In™ expression vector ensures high-level expression of your gene of interest. The Flp-In™ T-REx™-293 Cell Line contains pFRT⁄lacZeo and pcDNA™6⁄TR (from the T-REx™ System) stably integrated. Co-transfection of the Flp-In™ Cell Lines with a Flp-In™ expression vector and the Flp recombinase vector, pOG44, results in targeted integration of the expression vector to the same locus in every cell, ensuring homogeneous levels of gene expression.
The Flp-In™-293 work well with Flp-In™ vectors that express a gene from the CMV promoter (e.g., pcDNA™5⁄FRT, pcDNA5⁄FRT⁄V5-His-TOPO®, and pSecTag⁄FRT⁄V5-His-TOPO®).
For Research Use Only. Not for use in diagnostic procedures.
Flp-In™ T-Rex™ 293
Contents & Storage
1 vial of 1 x 107 cells are supplied frozen in 1 ml of 90% complete medium and 10% DMSO. Cells must be stored in liquid nitrogen. Cells are guaranteed stable for 6 months when properly stored.