Gibco™

FreeStyle™ 293-F Cells

Catalog number:  R79007

Related applications:

Antibody & Cell Culture Production

| Transfection

| Protein Expression

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Description

The FreeStyle™ 293-F cell line is derived from the 293 cell line and is intended for use with the FreeStyle™ MAX 293 Expression System or FreeStyle™ 293 Expression System. FreeStyle™ 293-F cells are adapted to suspension culture in FreeStyle™ 293 Expression Medium. Frozen cells are supplied in and may be thawed directly into FreeStyle™ 293 Expression Medium. The FreeStyle™ 293-F cell line is supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in 90% FreeStyle™ 293 Expression Medium and 10% DMSO. Characteristics of FreeStyle™ 293-F cells:

• Prepared from low-passage Master Cell Bank cultures derived from parental 293-F cells that were re-cloned by limiting dilution. The 293 clonal derived cultures are maintained in serum-free conditions for only 30 to 35 total passages.
• Adapted to high-density, serum-free, suspension growth and may be maintained in FreeStyle™ 293 Expression Medium.
• Demonstrate high transfection efficiencies with FreeStyle™ MAX reagent.
• Suspension cultures may be transfected in FreeStyle™ 293 Expression Medium without the need to change media.
• Permits transfection of cells at large volumes.

Specifications

Product Type: 293-F Cell
Quantity: 1 mL
Cell Line: FreeStyle™ 293-F Cells
Media Adaptation: FreeStyle™ 293 Expression Medium
System Type: FreeStyle™
Product Line: Freestyle™
For Use With (Application): Protein Expression and Transfection
Species: Human
Cell Type: 293-F

Contents & storage

1 vial of 1 x 107 cells. Store in liquid nitrogen.