GripTite™ 293 MSR Cell Line

Catalog number: R79507

Invitrogen™  Related applications: Antibody & Cell Culture Production | Mammalian Cell Culture | Protein Expression | Transfection

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Description

The GripTite™ 293 MSR Cell Line is a genetically engineered Human Embryonic Kidney (HEK 293) cell line that expresses the human macrophage scavenger receptor and strongly adheres to standard tissue culture plates for dependable results. Developed from a 293-H subclone, GripTite™ 293 MSR cells show the same fast cell growth, high transfection efficiency, and high-level expression characteristics of the parental 293-H cells. Unlike most 293 cells, GripTite® 293 MSR cells dont wash away during the repeated manipulations of routine tissue culture or plate washing protocols (Figure 1). Their superior adherence enables reliable reproduction of ligand-binding, enzymatic, or immunofluorescence assay results.

Use in High-Throughput Applications
The GripTite™ 293 MSR cells enhance performance in high-throughput cell-based, as well as standard tissue culture, applications. Uncontrolled cell loss during high-throughput protocols leads to unreliable results and expensive, time-consuming repeat experiments. Since GripTite™ 293 MSR cells adhere to standard tissue culture treated plastic, theres no need for the costly poly-lysine coated plates typically used in high-throughput analysis. The GripTite™ 293 MSR cells withstand automated plate washing and protocols using liquid handling robots on standard tissue culture plates without significant cell loss (Figure 2).
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Species: Human
Cell Line: 293 GripTite™ MSR
Product Size: 1 kit
System: GripTite™

Contents & storage

The GripTite™ 293 MSR Cell Line is provided as two tubes of a frozen stock of 1 x 107 cells, each in 1 ml of 90% complete media and 10% DMSO. Store in liquid nitrogen. Liquid Geneticin® Selective Antibiotic (20 ml) is supplied as a 50 mg/ml solution. Store at -20°C or 2°C to 8°C. Versene (100 ml) contains 0.2 g/L EDTA 4Na in phosphate-buffered saline. 1X concentration is 0.2 g/L=0.53 mM. Store at 2°C to 8°C. Guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols