Zitierungen und Referenzen (5)

Invitrogen™

ProBond™ Nickel-Chelating Resin

Name: ProBond™ Nickel-Chelating Resin
SKU: R80115
Price: 2.475.00 EUR

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Katalognummer	Menge
R80115	150 ml
R80101	50 ml

2 Optionen

Katalognummer R80115

Preis (EUR)

2.475,00

Each

Menge:

150 ml

Großbestellung oder individuelle Größe anfordern

Preis (EUR)

2.475,00

Each

ProBond™ Nickel-chelatbildendes Harz ist ein nickelbeladenes Affinitätsharz zur Aufreinigung rekombinanter Proteine, die eine Polyhistidin-Sequenz (6xHis) enthalten. An das Harz gebundene Proteine können entweder mit einem Puffer mit niedrigem pH-Wert oder durch Konkurrenz mit Imidazol oder Histidin eluiert werden. Die einstufige Reinigung kann sowohl unter nativen als auch unter denaturierenden Bedingungen durchgeführt werden. Das ProBond™-Harz enthält chelatbildende Iminodiessigsäure (NTA), die an ein stark vernetztes 6 %iges Agaroseharz gekoppelt ist, das sich für den Einsatz in FPLC-, Batch- und schwerkraftbasierten Anwendungen eignet.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

Menge150 ml

Stationäre PhaseNickel-chelatbildend

SäulentypAffinitätssäule

FormSuspension

ProduktlinieProBond

TypHarz-

Unit SizeEach

Inhalt und Lagerung

Das ProBond™ Harz ist vorgeladen und kann 1 bis 5 mg rekombinantes Protein pro 1 ml Harz binden. Es wird als 50%ige Suspension in 20% Ethanol bereitgestellt. Das Harz erscheint blau, wenn es mit Ni²⁺ geladen wird. Bei +4 °C lagern. ProBond™ Harz ist bei ordnungsgemäßer Lagerung garantiert 6 Monate lang stabil.

Häufig gestellte Fragen (FAQ)

How do you typically detect expression of a recombinant fusion protein?

Western blot analysis is typically used to detect the expressed protein. We sell several antibodies against various epitopes, such as Xpress, HisG, V5, or C-terminal 6xHis. Additionally, His-tagged proteins can be purified using our ProBond Purification System via affinity purification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What expression levels can be expected with the pTrcHis/CAT construct?

In one experiment, 35 µg CAT/mg total protein or 68.2 mg CAT/liter of culture was obtained. 50 ml of cell extract was loaded onto a ProBond column and 2 mg of CAT was recovered. The eluted protein appeared reasonably pure on a gel.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

I purified my protein from a ProBond column using denaturing conditions. After elution, I tried digesting off my N- terminal tag with EKMax Enterokinase, but see no EK cleavage. What can you suggest I try?

The enzyme could be denatured. Try buffer exchange or dialysis before digestion with EKMax Enterokinase.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

The pH of my ProBond buffers is off. Instead of pH 4-7, it is close to pH 9. What should I do?

pH drift is typical with these buffers. Adjust with concentrated HCl if the pH is too high or with 10 N NaOH if the pH is low.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

View all ProBond™ Nickel-Chelating Resin, 150 mL FAQs

Zitierungen und Referenzen (5)

Zitierungen und Referenzen

Abstract

Total synthesis of cyclic ADP-carbocyclic-ribose, a stable mimic of Ca2+-mobilizing second messenger cyclic ADP-ribose.

Authors: Shuto S; Fukuoka M; Manikowsky A; Ueno Y; Nakano T; Kuroda R; Kuroda H; Matsuda A;

Journal:J Am Chem Soc

PubMed ID:11535079

'The synthesis of cyclic ADP-carbocyclic-ribose (cADPcR, 4) designed as a stable mimic of cyclic ADP-ribose (cADPR, 1), a Ca2+-mobilizing second messenger, was achieved using as the key step a condensation reaction with the phenylthiophosphate-type substrate 14 to form an intramolecular pyrophosphate linkage. The N-1-carbocyclic-ribosyladenosine derivative 16 was prepared via the ... More

Interaction between the insulin receptor and its downstream effectors. Use of individually expressed receptor domains for structure/function analysis.

Authors:Paz K, Voliovitch H, Hadari YR, Roberts CT Jr, LeRoith D, Zick Y

Journal:J Biol Chem

PubMed ID:8636129

'A structural analysis has been carried out to determine which part of the intracellular domain of the insulin receptor (IR) beta subunit is involved in direct interaction with the receptor substrates IRS-1 and Shc. Toward this end, the juxtamembrane (JM) domain (amino acids 943- 984) and the carboxyl-terminal (CT) region ... More

A Calcium-Responsive Transcription Factor, CaRF, that Regulates Neuronal Activity-Dependent Expression of BDNF.

Authors: Tao Xu; West Anne E; Chen Wen G; Corfas Gabriel; Greenberg Michael E;

Journal:Neuron

PubMed ID:11832226

'Transcription of the brain-derived neurotrophic factor (BDNF) gene is regulated in a calcium- and neuron-selective manner; however, the mechanisms that underlie this selectivity are not known. We have characterized a new calcium-response element, CaRE1, that is required for activity-dependent transcription of BDNF exon III and have cloned a transcription factor, ... More

Tryptophan fluorescence reports nucleotide-induced conformational changes in a domain of the ArsA ATPase.

Authors:Zhou T, Rosen BP

Journal:J Biol Chem

PubMed ID:9242630

The ars operon of plasmid R773 encodes an ATP-dependent extrusion pump for arsenite and antimonite in Escherichia coli. The ArsA ATPase is the catalytic subunit of the pump protein, with two nucleotide binding consensus sequences, one in the NH2-terminal half and one in the COOH- terminal half of the protein. ... More

Structure and ligand of a histone acetyltransferase bromodomain.

Authors:Dhalluin C, Carlson JE, Zeng L, He C, Aggarwal AK, Zhou MM

Journal:Nature

PubMed ID:10365964

Histone acetylation is important in chromatin remodelling and gene activation. Nearly all known histone-acetyltransferase (HAT)-associated transcriptional co-activators contain bromodomains, which are approximately 110-amino-acid modules found in many chromatin-associated proteins. Despite the wide occurrence of these bromodomains, their three-dimensional structure and binding partners remain unknown. Here we report the solution structure ... More