Agarosa Ni-NTA
Agarosa Ni-NTA
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Citas y referencias (4)
Invitrogen™

Agarosa Ni-NTA

La agarosa Ni-NTA es una resina de afinidad cargada de níquel que se puede utilizar para purificar proteínas recombinantes queMás información
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Número de catálogoCantidad
R9010110 mL
R9011525 ml
R90110100 mL
Número de catálogo R90101
Precio (MXN)
-
Cantidad:
10 mL
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La agarosa Ni-NTA es una resina de afinidad cargada de níquel que se puede utilizar para purificar proteínas recombinantes que contienen una secuencia de polihistidina (6xHis). Las proteínas ligadas a la resina pueden eluirse con tampón de pH bajo o por competencia con imidazol o histidina. La purificación en un solo paso se puede realizar tanto en condiciones nativas como desnaturalizantes. Ni-NTA utiliza el ácido nitrilotriacético (NTA) del ligando quelante unido a una resina de agarosa al 6 % entrecruzada que es adecuada para su uso en aplicaciones de flujo por gravedad y lotes.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Cantidad10 mL
Fase estacionariaNi-NTA
Tipo de columnaColumna de afinidad
FormularioSuspensión
TipoAgarosa
Unit SizeEach
Contenido y almacenamiento
La resina Ni-NTA está precargada y es capaz de unir hasta 50 mg de proteína recombinante por cada 1 ml de resina. Se proporciona como una pulpa al 50 % en etanol al 30 %. La resina aparecerá de color azul cuando se cargue con Ni2+. Almacenar a +4 °C. Se garantiza la estabilidad de la resina Ni-NTA durante 6 meses si se almacena correctamente.

Preguntas frecuentes

Are there other sequences that can bind to nickel more tightly than 6xHis-tagged proteins and how can they be eluted.

Yes, 7xHis-tagged proteins, proteins naturally high in histidine, and other combinations of His and other amino acids will bind. To elute them, you have to increase the concentration of imidazole. Generally these peptides will not contaminate your fraction since they remain on the column. However after multiple uses of the same column, these peptides may reduce the binding capacity of the column.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can ProBond or Ni-NTA beads be used for large-scale preparations?

ProBond and Ni-NTA beads can be used in FPLC columns. However, the beads can only withstand low pressure (~43.5 psi max).

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

What should the typical protein recovery be when using the Probond Purification System or Ni-NTA Purification system?

Both systems are qualified by purifying 2 mg of myoglobin protein on a column and performing a Bradford assay. Protein recovery must be 75% or higher.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (4)

Citations & References
Abstract
A protein kinase encoded by the t complex responder gene causes non-mendelian inheritance
Authors:BERNHARD G. HERRMANN, BIRGIT KOSCHORZ, KARIN WERTZ, K. JOHN MCLAUGHLIN* & ANDREAS KISPERT
Journal:Nature
PubMed ID:10647005
'Males heterozygous for the t-haplotype form of mouse chromosome 17 preferentially transmit the t-chromosome to their progeny. Several distorter/sterility loci carried on the t-haplotype together impair flagellar function in all spermatozoa whereas the responder, Tcr, rescues t-sperm but not wild-type sperm. Thus, t-sperm have anadvantage over wild-type sperm in fertilizing ... More
DNA Polymerase lambda, a Novel DNA Repair Enzyme in Human Cells.
Authors: Garcia-Diaz Miguel; Bebenek Katarzyna; Sabariegos Rosario; Dominguez Orlando; Rodriguez Josana; Kirchhoff Tomas; Garcia-Palomero Esther; Picher Angel J; Juarez Raquel; Ruiz Jose F; Kunkel Thomas A; Blanco Luis;
Journal:J Biol Chem
PubMed ID:11821417
'DNA polymerase lambda (pol lambda) is a novel family X DNA polymerase that has been suggested to play a role in meiotic recombination and DNA repair. The recent demonstration of an intrinsic 5''-deoxyribose-5-phosphate lyase activity in pol lambda supports a function of this enzyme in base excision repair. However, the ... More
A ribozyme that ligates RNA to protein.
Authors: Baskerville Scott; Bartel David P;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12077317
'We have used a combination of in vitro selection and rational design to generate ribozymes that form a stable phosphoamide bond between the 5'' terminus of an RNA and a specific polypeptide. This reaction differs from that of previously identified ribozymes, although the product is analogous to the enzyme-nucleotidyl intermediates ... More
Transduction of MIN6 beta Cells with TAT-Syntaxin SNARE Motif Inhibits Insulin Exocytosis in Biphasic Insulin Release in a Distinct Mechanism Analyzed by Evanescent Wave Microscopy.
Authors: Ohara-Imaizumi Mica; Nakamichi Yoko; Nishiwaki Chiyono; Nagamatsu Shinya;
Journal:J Biol Chem
PubMed ID:12393909
To investigate the in vivo interaction of syntaxin-mediated soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) assembly and insulin exocytosis in biphasic release, we examined the dynamics of insulin granule motion such as docking and fusion with the plasma membrane when the syntaxin SNARE motif (H3 domain) was transduced into ... More