Ni-NTA Agarose

Catalog number:  R90101

 Related applications: Protein Purification & Isolation

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Ni-NTA Agarose is a nickel-charged affinity resin that can be used to purify recombinant proteins containing a polyhistidine (6xHis) sequence. Proteins bound to the resin may be eluted with either low pH buffer or by competition with imidazole or histidine. One-step purification can be performed under both native and denaturing conditions. Ni-NTA uses the chelating ligand nitrilotriacetic acid (NTA) coupled to a cross-linked 6% agarose resin that is suitable for use in batch and gravity flow applications.
For Research Use Only. Not for use in diagnostic procedures.


Bulk or Custom Form: Bulk Custom Form 1
Column Type: Affinity Column
Format: Suspension
Immobilized Phase: Ni-NTA
Product Size: 10 mL

Contents & storage

The Ni-NTA resin is pre-charged and able to bind up to 50 mg of recombinant protein per 1 ml of resin. It is provided as a 50% slurry in 30% ethanol. The resin will appear blue in color when charged with Ni2+ . Store at +4°C. Ni-NTA resin is guaranteed stable for 6 months when properly stored.


Manuals & protocols