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Invitrogen
Human NADK (aa 136-222) Control Fragment Recombinant Protein
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Product Details
RP-106458
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
VYVEKKVLEDPAIASDESFGAVKKKFCTFREDYDDISNQIDFIICLGGDGTLLYASSLFQGSVPPVMAFHLGSLGFLTPFSFENFQS
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (95%), Rat (95%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-65779. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
NADK (Nicotinamide Adenine Dinucleotide Kinase) is an enzyme crucial in the regulation of intracellular NADP+ levels, which are necessary for metabolic processes such as fatty acid and DNA synthesis. The human NADK gene is located on chromosome 1p36.33 and encodes a protein that primarily functions in the cytoplasm. NADK catalyzes the phosphorylation of NAD+ to NADP+, playing a significant role in maintaining the cellular redox state. Structurally, human NADK forms a dimeric organization, as revealed by its crystal structure, and includes four subunits (chains A-D) with specific colors indicating different chains. The enzyme's activity is regulated post-translationally, but its pathophysiological significance in humans remains underexplored. Multiple orthologs of the NADK gene are present in various organisms, illustrating its evolutionary conservation and importance across different species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ13052; NAD kinase; Poly(P)/ATP NAD kinase; ppnk
Gene Aliases: dJ283E3.1; NADK
UniProt ID: (Human) O95544
Entrez Gene ID: (Human) 65220
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