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Invitrogen
Human ZNF554 (aa 241-323) Control Fragment Recombinant Protein
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Product Details
RP-107232
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
GNHLCGSELDITSLASDSVLNHHQLGYADRRPCESNECGNAIRQNSHFIQHGGKMFVYLENGQSLNHGMALTIHNKINTAEKP
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (34%), Rat (34%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-111564. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
ZNF554, or Zinc Finger Protein 554, is a member of the Kruppel-associated box domain-zinc finger proteins (KRAB-ZNF) subfamily, residing on chromosome 19p13. This region is characterized by a concentration of heterochromatin proteins, which indicates tightly regulated gene expression. ZNF554 is implicated in various cellular processes including transcription regulation and apoptosis. Dysregulation of ZNF554 has been linked to several diseases; notably, its decreased expression is associated with poor prognosis in gliomas, specifically grades III and IV astrocytomas, where it affects the PI3K-Akt signaling pathway. It also plays a role in placental development, with dysfunction contributing to conditions such as fetal growth restriction (FGR). Additionally, ZNF554 transcription is influenced by factors like ZEB1, ZEB2, and hypoxic conditions, with neighboring hypoxia-response elements showing hypermethylation in preeclampsia. This gene's expression can significantly alter the transcriptional landscape, with overexpression leading to the differential regulation of hundreds of genes, underscoring its importance in cellular regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ34817; unnamed protein product
UniProt ID: (Human) Q86TJ5
Entrez Gene ID: (Human) 115196
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