RPCI-23 MM BAC CLONE - FAQs

How do I find/order an individual BAC/PAC clone?

There are several internet sites that may be used to locate BAC/PAC clones. One of them is listed below:

- Go here.
- Select the species of interest from the “Select organism” box.
- Type the feature, location or gene name in the “Search in genome” box on the right.
- As an example, choose “Search in genome”, enter “ITGA5” as the “Feature name” and click the search icon.
- Click on “Tracks shown”.
- Find the Clone library of interest, click on “Genomic Clones” and then select the box for the library of interest: for example: RP23 (RPCI-23 Female (C57BL/6J) Mouse BAC Library) and click “Configure”.
- In the refreshed window, you will see a list of BAC/PAC clones containing the ITGA5 gene (for example: RP23-427P19 where the Clone ID is 427P19).
- Go to CloneRanger, select the “BAC Mouse RP23” Collection, type in the Clone ID (in this case, 427P19), and click “Submit” to see if we carry the BAC clone. If the order is being placed by phone, provide the Cat No. (RPCI23.C) and the Clone address (in this case, RP23-427P19).