Human Melanocyte Growth Supplement (HMGS)

Catalog number: S0025

Gibco™  Related applications: Mammalian Cell Culture | Primary Cell Culture

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Description

A sterile, concentrated (100X) solution intended for use with Medium 254 or Medium 254CF to culture human epidermal melanocytes. Each 5 ml bottle of HMGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of human epidermal melanocytes and is the correct amount of supplement for a 500 ml bottle of basal Medium 254 or Medium 254CF.

When a 500 ml bottle of medium is supplemented with HMGS, the final concentrations of the components in the supplemented medium are:

• Bovine pituitary extract (BPE) (0.2% v/v)
• Fetal bovine serum (0.5% v/v)
• Recombinant human insulin-like growth factor-I (1 µg/ml)
• Bovine transferrin (5 µg/ml)
• Basic fibroblast growth factor (3 ng/ml)
• Hydrocortisone (0.18 µg/ml)
• Heparin (3 µg/ml)
• Phorbol 12-myristate 13-acetate (PMA) (10 ng/ml)
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type: Melanocytes
Product Size: 5 mL
Species: Human
Shipping Condition: Dry Ice

Contents & storage

Store at -20°C.

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Manuals & protocols

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