Informational:The SiteClick Antibody Labeling System is also available as the SiteClick™ Antibody Azido Modification Kit and compatible with small molecule SiteClick™ sDIBO Alkyne Kits
Invitrogen™

SiteClick™ Antibody Labeling Kits

Catalog number: S10454
Invitrogen™

SiteClick™ Antibody Labeling Kits

Catalog number: S10454
Generate perfectly labeled antibodies with the SiteClick R-PE, Qdot, or Biotin Antibody Labeling Kits. Site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies, regardless of isotype and host species. Depending upon the label, the resulting SiteClick R-PE, Qdot, or Biotin conjugated antibody can be used in flow cytometry, fluorescence imaging, or Western blot detection. Each kit contains everything needed to label 100 μg of IgG antibody with an easy-to-follow, step-by-step protocol. Achieve highly efficient, site-specific, and reproducible labeling with the SiteClick R-PE, Qdot, or Biotin Antibody Labeling Kits.
 
Catalog Number
S10454
Unit Size
1 kit
Label or Dye
Qdot 705
Excitation/Emission
405/705
Labeling Scale
100 μg
Price (USD)
Price: 576.00
Online price: 552.65
Your price:
Availability
-
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission405/705
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 705
ColorNear-IR
Labeling TargetIgG
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow, Red
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Qdot 655
Excitation/Emission
405/655
Labeling Scale
100 μg
Price (USD)
Price: 582.00
Online price: 558.65
Your price:
Availability
-
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission405/655
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 655
ColorFar-Red
Labeling TargetIgG
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
R-PE (R-Phycoerythrin)
Excitation/Emission
496, 546, 565/578
Labeling Scale
100 μg
Price (USD)
Price: 536.00
Online price: 514.65
Your price:
Availability
-
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission496, 546, 565/578
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineSiteClick™
Label or DyeR-PE (R-Phycoerythrin)
ColorRed-Orange
Labeling TargetIgG
Flow Cytometer Laser LinesBlue, Green, Yellow
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Qdot 525
Excitation/Emission
405/525
Labeling Scale
100 μg
Price (USD)
Price: 574.00
Online price: 560.65
Your price:
Availability
-
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission405/525
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 525
ColorGreen
Labeling TargetIgG
Filter TypeGFP, Qdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Qdot 565
Excitation/Emission
405/565
Labeling Scale
100 μg
Price (USD)
Price: 572.00
Online price: 549.65
Your price:
Availability
-
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission405/565
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 565
ColorGreen
Labeling TargetIgG
Filter TypeQdot 525-800, TRITC
Flow Cytometer Laser LinesUV, Violet, Blue, Green
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Qdot 585
Excitation/Emission
405/585
Labeling Scale
100 μg
Price (USD)
Price: 570.00
Online price: 547.65
Your price:
Availability
-
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission405/585
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 585
ColorOrange
Labeling TargetIgG
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Qdot 625
Excitation/Emission
405/625
Labeling Scale
100 μg
Price (USD)
Price: 582.00
Online price: 558.65
Your price:
Availability
-
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission405/625
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 625
ColorFar-Red
Labeling TargetIgG
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Qdot 800
Excitation/Emission
Labeling Scale
100 μg
Price (USD)
Price: 584.00
Online price: 560.65
Your price:
Availability
-
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 800
ColorNear-IR
Labeling TargetIgG
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow, Red
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Qdot 605
Excitation/Emission
405/605
Labeling Scale
100 μg
Price (USD)
Price: 578.00
Online price: 554.65
Your price:
Availability
-
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission405/605
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 605
ColorRed
Labeling TargetIgG
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Biotin
Excitation/Emission
Labeling Scale
100 to 250 μg
Price (USD)
Price: 570.00
Online price: 546.65
Your price:
Availability
-
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody
Product TypeLabeling Kit
Labeling MethodSiteClick Labeling System
Labeling Scale100 to 250 μg
Shipping ConditionWet Ice
Product LineSiteClick™
Label or DyeBiotin
Labeling TargetAntibodies
Unit Size1 kit
Showing 10 of 10
Catalog NumberSpecificationsUnit SizeLabel or DyeExcitation/EmissionLabeling ScalePrice (USD)AvailabilityQuantity
S10454Full specifications
1 kitQdot 705405/705100 μg
Price: 576.00
Online price: 552.65
Your price:
-
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission405/705
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 705
ColorNear-IR
Labeling TargetIgG
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow, Red
Unit Size1 kit
S10453Full specifications
1 kitQdot 655405/655100 μg
Price: 582.00
Online price: 558.65
Your price:
-
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission405/655
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 655
ColorFar-Red
Labeling TargetIgG
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
S10467Full specifications
1 kitR-PE (R-Phycoerythrin)496, 546, 565/578100 μg
Price: 536.00
Online price: 514.65
Your price:
-
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission496, 546, 565/578
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineSiteClick™
Label or DyeR-PE (R-Phycoerythrin)
ColorRed-Orange
Labeling TargetIgG
Flow Cytometer Laser LinesBlue, Green, Yellow
Unit Size1 kit
S10449Full specifications
1 kitQdot 525405/525100 μg
Price: 574.00
Online price: 560.65
Your price:
-
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission405/525
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 525
ColorGreen
Labeling TargetIgG
Filter TypeGFP, Qdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue
Unit Size1 kit
S10450Full specifications
1 kitQdot 565405/565100 μg
Price: 572.00
Online price: 549.65
Your price:
-
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission405/565
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 565
ColorGreen
Labeling TargetIgG
Filter TypeQdot 525-800, TRITC
Flow Cytometer Laser LinesUV, Violet, Blue, Green
Unit Size1 kit
S10451Full specifications
1 kitQdot 585405/585100 μg
Price: 570.00
Online price: 547.65
Your price:
-
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission405/585
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 585
ColorOrange
Labeling TargetIgG
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
S10452Full specifications
1 kitQdot 625405/625100 μg
Price: 582.00
Online price: 558.65
Your price:
-
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission405/625
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 625
ColorFar-Red
Labeling TargetIgG
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
S10455Full specifications
1 kitQdot 800100 μg
Price: 584.00
Online price: 560.65
Your price:
-
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 800
ColorNear-IR
Labeling TargetIgG
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow, Red
Unit Size1 kit
S10469Full specifications
1 kitQdot 605405/605100 μg
Price: 578.00
Online price: 554.65
Your price:
-
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody.
Product TypeLabeling Kit
Excitation/Emission405/605
Labeling MethodSiteClick Labeling System
Labeling Scale100 μg
Shipping ConditionWet Ice
Product LineQdot™, SiteClick™
Label or DyeQdot 605
ColorRed
Labeling TargetIgG
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
S20033Full specifications
1 kitBiotin100 to 250 μg
Price: 570.00
Online price: 546.65
Your price:
-
Kit ContentsContains everything needed to azido modify and label 100 μg of antibody
Product TypeLabeling Kit
Labeling MethodSiteClick Labeling System
Labeling Scale100 to 250 μg
Shipping ConditionWet Ice
Product LineSiteClick™
Label or DyeBiotin
Labeling TargetAntibodies
Unit Size1 kit
Showing 10 of 10

Conventional amine or thiol labeling chemistry can result in labeling of the antigen binding domain or compromise the structure of an antibody. SiteClick labeling technology, however, attaches the label under mild conditions to the heavy chains of an IgG antibody, ensuring that the antigen binding domains remain available for binding to your antigen target. Because SiteClick Labeling Kits allow simple and gentle site-selective attachment of detection molecules to heavy chain N-linked glycans — far from the antigen-binding domain — excellent reproducibility is achieved during sequential labelings, as well from antibody to antibody. No harsh reduction steps are required, and antibody labeling is consistent and reproducible each time it is performed.

The SiteClick R-PE, Qdot, or Biotin Antibody labeling workflow consists of three steps:

1. antibody carbohydrate domain modification,
2. azide attachment to the antibody and
3. conjugation with the DIBO-modified label.

This workflow employs copper-free click chemistry to covalently link the label containing the DIBO moiety with the azide-modified antibody. Labeling antibodies with the SiteClick antibody labeling system gives you confidence that the antibody will be labeled the same way every time, with no need for reaction optimization. This means the same number of dyes are attached to each molecule, the antigen binding site is preserved, and the same results are achieved every time.

Important features of the SiteClick Qdot Antibody Labeling Kits:

• Include everything required to label 100 μg of IgG antibody (100–250 μg of IgG antibody with the SiteClick Biotin Antibody Labeling Kit)
• Easy to follow step-by-step protocol that require only a few hours hands-on time to complete.
• Highly efficient, site-specific, reproducible labeling chemistry results in high quality antibody conjugates.

For Research Use Only. Not for use in diagnostic procedures.

Contents & Storage

Store the entire kit at 2 to 6°C upon receipt

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