Invitrogen™

SiteClick™ Antibody Labeling Kits

Catalog number: S10469
Invitrogen™

SiteClick™ Antibody Labeling Kits

Catalog number: S10469
Generate perfectly labeled antibodies with the SiteClick R-PE, Qdot, or Biotin Antibody Labeling Kits. Site selectivity is achieved by targeting the carbohydrate domains present on essentially all IgG antibodies, regardless of isotype and host species. Depending upon the label, the resulting SiteClick R-PE, Qdot, or Biotin conjugated antibody can be used in flow cytometry, fluorescence imaging, or Western blot detection. Each kit contains everything needed to label 100 µg of IgG antibody with an easy-to-follow, step-by-step protocol. Achieve highly efficient, site-specific, and reproducible labeling with the SiteClick R-PE, Qdot, or Biotin Antibody Labeling Kits.
 
Catalog Number
S10469
Unit Size
1 kit
Label or Dye
Qdot 605
Excitation
405, <605
Emission
605
Labeling Scale
100 µg
Price (USD)
471.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <605
Emission605
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 605
ColorRed
Labeling TargetAntibodies (IgG)
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Qdot 655
Excitation
405, <655
Emission
655
Labeling Scale
100 µg
Price (USD)
472.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <655
Emission655
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 655
ColorFar-Red
Labeling TargetAntibodies (IgG)
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
R-PE
Excitation
496, 546, 565
Emission
578
Labeling Scale
100 µg
Price (USD)
436.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation496, 546, 565
Emission578
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineSiteClick
Label or DyeR-PE
ColorRed-Orange
Labeling TargetAntibodies (IgG)
Flow Cytometer Laser LinesBlue, Green, Yellow
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Qdot 525
Excitation
405, <525
Emission
525
Labeling Scale
100 µg
Price (USD)
467.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <525
Emission525
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 525
ColorGreen
Labeling TargetAntibodies (IgG)
Filter TypeGFP, Qdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Qdot 705
Excitation
405, <705
Emission
705
Labeling Scale
100 µg
Price (USD)
469.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <705
Emission705
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 705
ColorNear-IR
Labeling TargetAntibodies (IgG)
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow, Red
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Qdot 565
Excitation
405, <565
Emission
565
Labeling Scale
100 µg
Price (USD)
464.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <565
Emission565
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 565
ColorGreen
Labeling TargetAntibodies (IgG)
Filter TypeQdot 525-800, TRITC
Flow Cytometer Laser LinesUV, Violet, Blue, Green
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Qdot 585
Excitation
405, <585
Emission
585
Labeling Scale
100 µg
Price (USD)
463.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <585
Emission585
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 585
ColorOrange
Labeling TargetAntibodies (IgG)
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Qdot 625
Excitation
405, <625
Emission
625
Labeling Scale
100 µg
Price (USD)
473.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <625
Emission625
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 625
ColorFar-Red
Labeling TargetAntibodies (IgG)
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Qdot 800
Excitation
405, <800
Emission
800
Labeling Scale
100 µg
Price (USD)
474.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <800
Emission800
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 800
ColorNear-IR
Labeling TargetAntibodies (IgG)
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow, Red
Unit Size1 kit
Catalog Number
Unit Size
1 kit
Label or Dye
Biotin
Excitation
Emission
Labeling Scale
100 - 250 µg
Price (USD)
462.00
Your Price:
Availability
***
Full specifications
Quantity
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Labeling MethodSiteClick Labeling System
Labeling Scale100 - 250 µg
Shipping ConditionWet Ice
Product LineSiteClick
Label or DyeBiotin
Labeling TargetAntibodies (General)
Unit Size1 kit
Showing 10 of 10
Catalog NumberSpecificationsUnit SizeLabel or DyeExcitationEmissionLabeling ScalePrice (USD)AvailabilityQuantity
S10469Full specifications
1 kitQdot 605405, <605605100 µg
471.00
Your Price:
***
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <605
Emission605
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 605
ColorRed
Labeling TargetAntibodies (IgG)
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
S10453Full specifications
1 kitQdot 655405, <655655100 µg
472.00
Your Price:
***
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <655
Emission655
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 655
ColorFar-Red
Labeling TargetAntibodies (IgG)
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
S10467Full specifications
1 kitR-PE496, 546, 565578100 µg
436.00
Your Price:
***
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation496, 546, 565
Emission578
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineSiteClick
Label or DyeR-PE
ColorRed-Orange
Labeling TargetAntibodies (IgG)
Flow Cytometer Laser LinesBlue, Green, Yellow
Unit Size1 kit
S10449Full specifications
1 kitQdot 525405, <525525100 µg
467.00
Your Price:
***
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <525
Emission525
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 525
ColorGreen
Labeling TargetAntibodies (IgG)
Filter TypeGFP, Qdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue
Unit Size1 kit
S10454Full specifications
1 kitQdot 705405, <705705100 µg
469.00
Your Price:
***
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <705
Emission705
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 705
ColorNear-IR
Labeling TargetAntibodies (IgG)
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow, Red
Unit Size1 kit
S10450Full specifications
1 kitQdot 565405, <565565100 µg
464.00
Your Price:
***
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <565
Emission565
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 565
ColorGreen
Labeling TargetAntibodies (IgG)
Filter TypeQdot 525-800, TRITC
Flow Cytometer Laser LinesUV, Violet, Blue, Green
Unit Size1 kit
S10451Full specifications
1 kitQdot 585405, <585585100 µg
463.00
Your Price:
***
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <585
Emission585
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 585
ColorOrange
Labeling TargetAntibodies (IgG)
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
S10452Full specifications
1 kitQdot 625405, <625625100 µg
473.00
Your Price:
***
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <625
Emission625
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 625
ColorFar-Red
Labeling TargetAntibodies (IgG)
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow
Unit Size1 kit
S10455Full specifications
1 kitQdot 800405, <800800100 µg
474.00
Your Price:
***
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody.
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Excitation405, <800
Emission800
Labeling MethodSiteClick Labeling System
Labeling Scale100 µg
Shipping ConditionWet Ice
Product LineQdot, SiteClick
Label or DyeQdot 800
ColorNear-IR
Labeling TargetAntibodies (IgG)
Filter TypeQdot 525-800
Flow Cytometer Laser LinesUV, Violet, Blue, Green, Yellow, Red
Unit Size1 kit
S20033Full specifications
1 kitBiotin100 - 250 µg
462.00
Your Price:
***
Kit ContentsContains everything needed to azido modify and label 100 µg of antibody
Storage RequirementsStore the entire kit at 2 to 6°C upon receipt
Labeling MethodSiteClick Labeling System
Labeling Scale100 - 250 µg
Shipping ConditionWet Ice
Product LineSiteClick
Label or DyeBiotin
Labeling TargetAntibodies (General)
Unit Size1 kit
Showing 10 of 10
Conventional amine or thiol labeling chemistry can result in labeling of the antigen binding domain or compromise the structure of an antibody. SiteClick labeling technology, however, attaches the label under mild conditions to the heavy chains of an IgG antibody, ensuring that the antigen binding domains remain available for binding to your antigen target. Because SiteClick Labeling Kits allow simple and gentle site-selective attachment of detection molecules to heavy chain N-linked glycans — far from the antigen-binding domain — excellent reproducibility is achieved during sequential labelings, as well from antibody to antibody. No harsh reduction steps are required, and antibody labeling is consistent and reproducible each time it is performed.

The SiteClick R-PE, Qdot, or Biotin Antibody labeling workflow consists of three steps:

1. antibody carbohydrate domain modification,
2. azide attachment to the antibody and
3. conjugation with the DIBO-modified label.

This workflow employs copper-free click chemistry to covalently link the label containing the DIBO moiety with the azide-modified antibody. Labeling antibodies with the SiteClick antibody labeling system gives you confidence that the antibody will be labeled the same way every time, with no need for reaction optimization. This means the same number of dyes are attached to each molecule, the antigen binding site is preserved, and the same results are achieved every time.

Important features of the SiteClick Qdot Antibody Labeling Kits:

• Include everything required to label 100 µg of IgG antibody (100–250 µg of IgG antibody with the SiteClick Biotin Antibody Labeling Kit)
• Easy to follow step-by-step protocol that require only a few hours hands-on time to complete.
• Highly efficient, site-specific, reproducible labeling chemistry results in high quality antibody conjugates.
For Research Use Only. Not for use in diagnostic procedures.

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Frequently asked questions (FAQs)

Citations & References