Zitierungen und Referenzen (8)

Streptavidin, Pacific Blue™ conjugate

Unser Streptavidin, Pacific Blue™ Konjugat ist eine helle, blau fluoreszierende Sonde mit Anregung/Emission bei ∼ 410/455 nm. Streptavidin ist einWeitere Informationen

Katalognummer	Menge
S11222	1 mg

Katalognummer S11222

Preis (EUR)

449,00

Menge:

Preis (EUR)

449,00

Unser Streptavidin, Pacific Blue™ Konjugat ist eine helle, blau fluoreszierende Sonde mit Anregung/Emission bei ∼ 410/455 nm. Streptavidin ist ein nicht glykosyliertes Biotin-bindendes Protein, das aus Bakterien isoliert ist und einen nahezu neutralen isoelektrischen Punkt hat. Es wird häufig verwendet, um biotinylierte Sonden nachzuweisen, da es Berichten zufolge weniger unspezifische Bindungen aufweist als das glykosylierte, Biotin-bindende Protein Avidin.

Sehen Sie sich eine Auswahlhilfe für alle Alexa Fluor™ Streptavidin- und Avidin-Konjugate an.

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.

Specifications

FarbeGelb

BeschreibungPacific Blue™ Konjugat Streptavidin, 1 mg, Gelb, Festform, 407 nm Absorptionsfähigkeit, Pacific Blue™ Marker oder Farbstoff, 410/455 nm Anregung/Emission, wird in Blot-Analyse, Durchflusszytometrie, histochemischen Anwendungen, Immunassays verwendet, Lagertemperatur -5 bis -30 °C

NachweisverfahrenFluoreszent

Anregung/Emission410/455 nm

Durchflusszytometer-Laserlinien405

Zur Verwendung mit (Anwendung)Durchflusszytometrie, Immunassays, histochemische Anwendungen, Blot-Analyse

ZusammensetzungFest

Marker oder FarbstoffPacific Blue

Verpackung1 x

ProdukttypStreptavidin-Konjugat

Reinheits- oder QualitätsgradTLC

Menge1 mg

KennzeichnungNur für Forschungszwecke (RUO)

Probenvolumen1 mg

Haltbarkeit6 Monate

VersandbedingungRaumtemperatur

Lagerungspuffer10 mg/ml PBS

KonjugatPacific Blue

FormFest

ProduktliniePacific Blue

Unit SizeEach

Inhalt und Lagerung

Enthält 1 Fläschchen Streptavidin Pacific Blue™ Konjugat.

Tiefgekühlt (bei -5 bis -30 °C), vor Licht geschützt lagern.

Häufig gestellte Fragen (FAQ)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

Zitierungen und Referenzen (8)

Zitierungen und Referenzen

Abstract

Arenavirus nucleoprotein targets interferon regulatory factor-activating kinase IKKe.

Authors:Pythoud C, Rodrigo WW, Pasqual G, Rothenberger S, Martínez-Sobrido L, de la Torre JC, Kunz S,

Journal:J Virol

PubMed ID:22532683

'Arenaviruses perturb innate antiviral defense by blocking induction of type I interferon (IFN) production. Accordingly, the arenavirus nucleoprotein (NP) was shown to block activation and nuclear translocation of interferon regulatory factor 3 (IRF3) in response to virus infection. Here, we sought to identify cellular factors involved in innate antiviral signaling ... More

In vivo expansion of T reg cells with IL-2-mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression.

Authors:Webster KE, Walters S, Kohler RE, Mrkvan T, Boyman O, Surh CD, Grey ST, Sprent J,

Journal:J Exp Med

PubMed ID:19332874

'Via a transcription factor, Foxp3, immunoregulatory CD4(+)CD25(+) T cells (T reg cells) play an important role in suppressing the function of other T cells. Adoptively transferring high numbers of T reg cells can reduce the intensity of the immune response, thereby providing an attractive prospect for inducing tolerance. Extending our ... More

Rapid lytic granule convergence to the MTOC in natural killer cells is dependent on dynein but not cytolytic commitment.

Authors:Mentlik AN, Sanborn KB, Holzbaur EL, Orange JS,

Journal:Mol Biol Cell

PubMed ID:20444980

'Natural killer cells are lymphocytes specialized to participate in host defense through their innate ability to mediate cytotoxicity by secreting the contents of preformed secretory lysosomes (lytic granules) directly onto a target cell. This form of directed secretion requires the formation of an immunological synapse and occurs stepwise with actin ... More

Immunosuppressive property within the Streptococcus pneumoniae cell wall that inhibits generation of T follicular helper, germinal center, and plasma cell response to a coimmunized heterologous protein.

Authors:Saumyaa, Arjunaraja S, Pujanauski L, Colino J, Torres RM, Snapper CM,

Journal:

PubMed ID:23817619

'We previously demonstrated that intact, inactivated Streptococcus pneumoniae (unencapsulated strain R36A) inhibits IgG responses to a number of coimmunized soluble antigens (Ags). In this study, we investigated the mechanism of this inhibition and whether other extracellular bacteria exhibited similar effects. No inhibition was observed if R36A was given 24 h ... More

Thymocytes may persist and differentiate without any input from bone marrow progenitors.

Authors:Peaudecerf L, Lemos S, Galgano A, Krenn G, Vasseur F, Di Santo JP, Ezine S, Rocha B,

Journal:J Exp Med

PubMed ID:22778388

'Thymus transplants can correct deficiencies of the thymus epithelium caused by the complete DiGeorge syndrome or FOXN1 mutations. However, thymus transplants were never used to correct T cell-intrinsic deficiencies because it is generally believed that thymocytes have short intrinsic lifespans. This notion is based on thymus transplantation experiments where it ... More

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