Streptavidin, Pacific Blue™ conjugate

Citas y referencias (8)

Streptavidin, Pacific Blue™ conjugate

Nuestra estreptavidina, conjugado Pacific Blue™ es una sonda fluorescente azul brillante con excitación ∼ 410/455 nm. La estreptavidina es unaMás información

Número de catálogo	Cantidad
S11222	1 mg

Número de catálogo S11222

Precio (MXN)

Cantidad:

Nuestra estreptavidina, conjugado Pacific Blue™ es una sonda fluorescente azul brillante con excitación ∼ 410/455 nm. La estreptavidina es una proteína no glucosilada de unión de biotina que se aísla de bacterias y tiene un punto isoeléctrico casi neutro. Se utiliza ampliamente para detectar sondas biotiniladas, porque se ha constatado que exhibe una unión menos inespecífica que la avidina, una proteína glucosilada de unión de biotina.

Consulte la guía de selección para ver todos los conjugados de estreptavidina y avidina Alexa Fluor™.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorAmarillo

DescripciónEstreptavidina, conjugado Pacific Blue™, 1 mg, amarillo, forma sólida, absorbancia de 407 nm, etiqueta o colorante Pacific Blue™, excitación/emisión de 410/455 nm, se utiliza en análisis de blotting, citometría de flujo, aplicaciones histoquímicas, inmunoensayos, temperatura de almacenamiento de –5 a –30 °C.

Método de detecciónFluorescente

Excitación/emisión410/455 nm

Líneas láser del citómetro de flujo405

Para utilizar con (aplicación)Citometría de flujo, inmunoensayos, aplicaciones histoquímicas, análisis de blot

FormulaciónMaciza

Etiqueta o tintePacific Blue

Envase1 unidad

Tipo de productoConjugado de estreptavidina

Grado de pureza o calidadTLC

Cantidad1 mg

Estado normativoPara uso exclusivo en investigación (RUO)

Volumen de muestra1 mg

Duración de almacenamiento6 meses

Condiciones de envíoTemperatura ambiente

Tampón de almacenamiento10 mg/ml de PBS

ConjugadoPacific Blue

FormularioSólido

Línea de productosPacific Blue

Unit SizeEach

Contenido y almacenamiento

Contiene 1 vial de conjugado de estreptavidina Pacific Blue™.

Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.

Preguntas frecuentes

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

Citations & References (8)

Citations & References

Abstract

Arenavirus nucleoprotein targets interferon regulatory factor-activating kinase IKKe.

Authors:Pythoud C, Rodrigo WW, Pasqual G, Rothenberger S, Martínez-Sobrido L, de la Torre JC, Kunz S,

Journal:J Virol

PubMed ID:22532683

'Arenaviruses perturb innate antiviral defense by blocking induction of type I interferon (IFN) production. Accordingly, the arenavirus nucleoprotein (NP) was shown to block activation and nuclear translocation of interferon regulatory factor 3 (IRF3) in response to virus infection. Here, we sought to identify cellular factors involved in innate antiviral signaling ... More

In vivo expansion of T reg cells with IL-2-mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression.

Authors:Webster KE, Walters S, Kohler RE, Mrkvan T, Boyman O, Surh CD, Grey ST, Sprent J,

Journal:J Exp Med

PubMed ID:19332874

'Via a transcription factor, Foxp3, immunoregulatory CD4(+)CD25(+) T cells (T reg cells) play an important role in suppressing the function of other T cells. Adoptively transferring high numbers of T reg cells can reduce the intensity of the immune response, thereby providing an attractive prospect for inducing tolerance. Extending our ... More

Rapid lytic granule convergence to the MTOC in natural killer cells is dependent on dynein but not cytolytic commitment.

Authors:Mentlik AN, Sanborn KB, Holzbaur EL, Orange JS,

Journal:Mol Biol Cell

PubMed ID:20444980

'Natural killer cells are lymphocytes specialized to participate in host defense through their innate ability to mediate cytotoxicity by secreting the contents of preformed secretory lysosomes (lytic granules) directly onto a target cell. This form of directed secretion requires the formation of an immunological synapse and occurs stepwise with actin ... More

Immunosuppressive property within the Streptococcus pneumoniae cell wall that inhibits generation of T follicular helper, germinal center, and plasma cell response to a coimmunized heterologous protein.

Authors:Saumyaa, Arjunaraja S, Pujanauski L, Colino J, Torres RM, Snapper CM,

Journal:

PubMed ID:23817619

'We previously demonstrated that intact, inactivated Streptococcus pneumoniae (unencapsulated strain R36A) inhibits IgG responses to a number of coimmunized soluble antigens (Ags). In this study, we investigated the mechanism of this inhibition and whether other extracellular bacteria exhibited similar effects. No inhibition was observed if R36A was given 24 h ... More

Thymocytes may persist and differentiate without any input from bone marrow progenitors.

Authors:Peaudecerf L, Lemos S, Galgano A, Krenn G, Vasseur F, Di Santo JP, Ezine S, Rocha B,

Journal:J Exp Med

PubMed ID:22778388

'Thymus transplants can correct deficiencies of the thymus epithelium caused by the complete DiGeorge syndrome or FOXN1 mutations. However, thymus transplants were never used to correct T cell-intrinsic deficiencies because it is generally believed that thymocytes have short intrinsic lifespans. This notion is based on thymus transplantation experiments where it ... More

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