Alexa Fluor™ 532 streptavidin
comprises a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Alexa Fluor™ dye). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays. Alexa Fluor™ dye streptavidin conjugates are supplied as 1 mg lyophilized product or in 0.5 mL volumes of a 2 mg/mL solution.Important Features of Alexa Fluor™ 532 Streptavidin Conjugates
• Alexa Fluor™ 532 streptavidin conjugate has Ex/Em maxima of ∼ (530/554)
• Bright, photostable fluorescence
• High solubility in aqueous solutions
• Available in multiple colors
• Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and moreProperties of Alexa Fluor™ Dyes
Alexa Fluor™ dyes are organic fluorescent dyes developed for better performance in imaging and other labeling protocols and exhibit improved photostability
and improved solubility in aqueous solutions. Available in a broad range of colors
, these dyes are a good choice for most imaging applications.Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit
to minimize this interference.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.Related Links: Learn more about Avidin-Biotin DetectionLearn more about Alexa Fluor™ DyesFind out about other Labeled Streptavidin ConjugatesRead Avidin and Streptavidin Conjugates-Section 7.6
in the Molecular Probes Handbook
For Research Use Only. Not for use in diagnostic procedures.