Tinción de ácido nucleico SYTO™ 60 Red Fluorescent - Solución de 5 mm en DMSO

Citas y referencias (25)

Invitrogen™

Tinción de ácido nucleico SYTO™ 60 Red Fluorescent - Solución de 5 mm en DMSO

La tinción de ácido nucleico rojo fluorescente permeable en células SYTO 60 exhibe fluorescencia rojo brillante al enlazarse con losMás información

Número de catálogo	Cantidad
S11342	250 μL

Número de catálogo S11342

Precio (MXN)

Cantidad:

250 μL

La tinción de ácido nucleico rojo fluorescente permeable en células SYTO 60 exhibe fluorescencia rojo brillante al enlazarse con los ácidos nucleicos. Debido a que el patrón de tinción de SYTO en las células vivas puede variar entre los diferentes tipos de células, ofrecemos el kit de muestreo para tinción de ácido nucleico rojo fluorescente SYTO (S-11340) para permitir a los investigadores encontrar el tinte más adecuado para su sistema.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorRojo

DescripciónTinción de ácido nucleico SYTO™ 60 Red Fluorescent - Solución de 5 mm en DMSO

Método de detecciónFluorescente

Tipo de colorantePermeabilidad celular

Emisión678 nm

Intervalo de longitud de onda de excitación652 nm

Para utilizar con (equipo)Microscopio de fluorescencia

Línea de productosSYTO

Cantidad250 μL

Condiciones de envíoTemperatura ambiente

Volumen (métrico)250 μl

Tipo de etiquetaFluorescente

Tipo de productoTinción de ácidos nucleicos

SubCellular LocalizationÁcidos nucleicos

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (25)

Citations & References

Abstract

The protein tyrosine phosphatase PTP-BL associates with the midbody and is involved in the regulation of cytokinesis.

Authors:Herrmann L, Dittmar T, Erdmann KS

Journal:Mol Biol Cell

PubMed ID:12529439

'PTP-BL is a highly modular protein tyrosine phosphatase of unknown function. It consists of an N-terminal FERM domain, five PDZ domains, and a C-terminally located tyrosine phosphatase domain. Here we show that PTP-BL is involved in the regulation of cytokinesis. We demonstrate localization of endogenous PTP-BL at the centrosomes during ... More

Nucleic acid binding agents exert local toxic effects on neurites via a non-nuclear mechanism.

Authors:Pin S, Chen H, Lein PJ, Wang MM

Journal:J Neurochem

PubMed ID:16441515

'The mechanism by which drugs that target nucleic acids cause neurotoxicity is not well described. We characterized the neurotoxicity of Hoechst 33342 (bis-benzimide), a common cell permeable nuclear dye, in primary neuronal cultures. The mechanism of cell death was not apoptotic, as death is rapid, not accompanied by typical nuclear ... More

Multiparameter detection of apoptosis using red-excitable SYTO probes.

Authors:Wlodkowic D, Skommer J, Hillier C, Darzynkiewicz Z,

Journal:Cytometry A

PubMed ID:18431792

'Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent ... More

Liver fatty acid-binding protein colocalizes with peroxisome proliferator activated receptor alpha and enhances ligand distribution to nuclei of living cells.

Authors:Huang H, Starodub O, McIntosh A, Atshaves BP, Woldegiorgis G, Kier AB, Schroeder F

Journal:Biochemistry

PubMed ID:14992586

'Although it is hypothesized that long-chain fatty acyl CoAs (LCFA-CoAs) and long-chain fatty acids (LCFAs) regulate transcription in the nucleus, little is known regarding factors that determine the distribution of these ligands to nuclei of living cells. Immunofluorescence colocalization showed that liver fatty acid-binding protein (L-FABP; binds LCFA-CoA as well ... More

Cytoplasmic and nuclear delivery of a TAT-derived peptide and a beta-peptide after endocytic uptake into HeLa cells.

Authors:Potocky TB, Menon AK, Gellman SH

Journal:J Biol Chem

PubMed ID:14517218

'Several short, highly cationic peptides are able to enter the cytoplasm and nucleus of cells from the extracellular medium. The mechanism of entry is unknown. A number of fluorescence-based studies suggested that these molecules cross the plasma membrane by an energy-independent process, directly gaining access to the cytoplasm. Recent reports ... More

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