SYTO™ 63 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

Citas y referencias (8)

Invitrogen™

SYTO™ 63 Red Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

La tinción de ácido nucleico rojo fluorescente permeable en células SYTO 63 exhibe fluorescencia rojo brillante al enlazarse con losMás información

Número de catálogo	Cantidad
S11345	250 μL

Número de catálogo S11345

Precio (MXN)

Cantidad:

250 μL

La tinción de ácido nucleico rojo fluorescente permeable en células SYTO 63 exhibe fluorescencia rojo brillante al enlazarse con los ácidos nucleicos. Debido a que el patrón de tinción de SYTO en las células vivas puede variar entre los diferentes tipos de células, ofrecemos el kit de muestreo para tinción de ácido nucleico rojo fluorescente SYTO (S-11340) para permitir a los investigadores encontrar el tinte más adecuado para su sistema.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorRojo

DescripciónTinción de ácido nucleico SYTO™ 63 Red Fluorescent - Solución de 5 mm en DMSO

Método de detecciónFluorescente

Tipo de colorantePermeabilidad celular

Emisión673 nm

Intervalo de longitud de onda de excitación657 nm

Para utilizar con (equipo)Microscopio de fluorescencia

Línea de productosSYTO

Cantidad250 μL

Condiciones de envíoTemperatura ambiente

Volumen (métrico)250 μl

Tipo de etiquetaFluorescente

Tipo de productoTinción de ácidos nucleicos

SubCellular LocalizationÁcidos nucleicos

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (8)

Citations & References

Abstract

Nucleic acid binding agents exert local toxic effects on neurites via a non-nuclear mechanism.

Authors:Pin S, Chen H, Lein PJ, Wang MM

Journal:J Neurochem

PubMed ID:16441515

'The mechanism by which drugs that target nucleic acids cause neurotoxicity is not well described. We characterized the neurotoxicity of Hoechst 33342 (bis-benzimide), a common cell permeable nuclear dye, in primary neuronal cultures. The mechanism of cell death was not apoptotic, as death is rapid, not accompanied by typical nuclear ... More

Multiparameter detection of apoptosis using red-excitable SYTO probes.

Authors:Wlodkowic D, Skommer J, Hillier C, Darzynkiewicz Z,

Journal:Cytometry A

PubMed ID:18431792

'Functional assays allowing phenotypic characterization of different cell death parameters at a single-cell level are important tools for preclinical anticancer drug screening. Currently, the selection of cytometric assays is limited by the availability of fluorescent probes with overlapping spectral characteristics. Following on our earlier reports on green and orange fluorescent ... More

Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry.

Authors:Day JP, Kell DB, Griffith GW

Journal:Appl Environ Microbiol

PubMed ID:11772606

'The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or ... More

High throughput single molecule detection for monitoring biochemical reactions.

Authors:Okagbare PI, Soper SA,

Journal:Analyst

PubMed ID:19082181

The design, performance and application of a novel optical system for high throughput single molecule detection (SMD) configured in a continuous flow format using microfluidics is reported. The system consisted of a microfabricated polymer-based multi-channel fluidic network situated within the optical path of a laser source (lambda(ex) = 660 nm) ... More

Cytoplasmic O-glycosylation prevents cell surface transport of E-cadherin during apoptosis.

Authors:Zhu W, Leber B, Andrews DW

Journal:EMBO J

PubMed ID:11689440

Cellular adhesion is regulated by members of the cadherin family of adhesion receptors and their cytoplasmic adaptor proteins, the catenins. Adhesion complexes are regulated by recycling from the plasma membrane and proteolysis during apoptosis. We report that in MCF-7, MDA-MB-468 and MDCK cells, induction of apoptosis by agents that cause ... More

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