SYTO™ 41 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
SYTO™ 41 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO
Citas y referencias (8)
Invitrogen™

SYTO™ 41 Blue Fluorescent Nucleic Acid Stain - 5 mM Solution in DMSO

La tinción de ácido nucleico azul fluorescente permeable en células SYTO 41 exhibe fluorescencia azul brillante al enlazarse con losMás información
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Número de catálogoCantidad
S11352250 μL
Número de catálogo S11352
Precio (MXN)
-
Cantidad:
250 μL
La tinción de ácido nucleico azul fluorescente permeable en células SYTO 41 exhibe fluorescencia azul brillante al enlazarse con los ácidos nucleicos. Debido a que el patrón de tinción de SYTO en las células vivas puede variar entre los diferentes tipos de células, ofrecemos el kit de muestreo para tinción de ácido nucleico azul fluorescente SYTO (S-11350) para permitir a los investigadores encontrar el tinte más adecuado para su sistema.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ColorAzul
DescripciónTinción de ácido nucleico SYTO™ 41 Blue Fluorescent: solución de 5 mM en DMSO
Método de detecciónFluorescente
Tipo de colorantePermeabilidad celular
Emisión454 nm
Intervalo de longitud de onda de excitación430 nm
Para utilizar con (equipo)Microscopio de fluorescencia
Línea de productosSYTO
Cantidad250 μL
Condiciones de envíoTemperatura ambiente
Volumen (métrico)250 μl
Tipo de etiquetaFluorescente
Tipo de productoTinción de ácidos nucleicos
SubCellular LocalizationÁcidos nucleicos
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (8)

Citations & References
Abstract
Nucleic acid binding agents exert local toxic effects on neurites via a non-nuclear mechanism.
Authors:Pin S, Chen H, Lein PJ, Wang MM
Journal:J Neurochem
PubMed ID:16441515
'The mechanism by which drugs that target nucleic acids cause neurotoxicity is not well described. We characterized the neurotoxicity of Hoechst 33342 (bis-benzimide), a common cell permeable nuclear dye, in primary neuronal cultures. The mechanism of cell death was not apoptotic, as death is rapid, not accompanied by typical nuclear ... More
Imaging collagen in intact viable healthy and atherosclerotic arteries using fluorescently labeled CNA35 and two-photon laser scanning microscopy.
Authors:Megens RT, Oude Egbrink MG, Cleutjens JP, Kuijpers MJ, Schiffers PH, Merkx M, Slaaf DW, van Zandvoort MA,
Journal:Mol Imaging
PubMed ID:17711780
We evaluated CNA35 as a collagen marker in healthy and atherosclerotic arteries of mice after both ex vivo and in vivo administration and as a molecular imaging agent for the detection of atherosclerosis. CNA35 conjugated with fluorescent Oregon Green 488 (CNA35/OG488) was administered ex vivo to mounted viable muscular (uterine), ... More
Two pathways converge at CED-10 to mediate actin rearrangement and corpse removal in C. elegans.
Authors:Kinchen JM, Cabello J, Klingele D, Wong K, Feichtinger R, Schnabel H, Schnabel R, Hengartner MO
Journal:Nature
PubMed ID:15744306
The removal of apoptotic cells is essential for the physiological well being of the organism. In Caenorhabditis elegans, two conserved, partially redundant genetic pathways regulate this process. In the first pathway, the proteins CED-2, CED-5 and CED-12 (mammalian homologues CrkII, Dock180 and ELMO, respectively) function to activate CED-10 (Rac1). In ... More
Two-photon microscopy of vital murine elastic and muscular arteries. Combined structural and functional imaging with subcellular resolution.
Authors:Megens RT, Reitsma S, Schiffers PH, Hilgers RH, De Mey JG, Slaaf DW, oude Egbrink MG, van Zandvoort MA
Journal:J Vasc Res
PubMed ID:17192719
Understanding vascular pathologies requires insight in the structure and function, and, hence, an imaging technique combining subcellular resolution, large penetration depth, and optical sectioning. We evaluated the applicability of two-photon laser-scanning microscopy (TPLSM) in large elastic and small muscular arteries under physiological conditions. Elastic (carotid) and muscular (uterine, mesenteric) arteries ... More
Loss of α-gal during primate evolution enhanced antibody-effector function and resistance to bacterial sepsis.
Authors:
Journal:Cell Host Microbe
PubMed ID:33497603
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