SYTO™ Orange Fluorescent Nucleic Acid Stain Sampler Kit - SYTO™ dyes 80-85 - 50 μL each

Incluye colorantes SYTO naranja fluorescente y con permeabilidad celular

Número de catálogo	Cantidad
S11360	1 kit

Número de catálogo S11360

Precio (MXN)

Cantidad:

1 kit

The SYTO Orange Fluorescent Nucleic Acid Stain Sampler Kit contains our collection of cell-permeant, orange-fluorescent SYTO nucleic acid stains. Because the dyes may demonstrate different staining behaviors with various tissues and cells, it may be necessary to test the dyes to find the optimal dye for a specific application. The kit contains 50 μL each of SYTO 80–85 dyes.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

ColorNaranja

DescripciónKit de muestreador de tinción de ácido nucleico fluorescente SYTO™ Orange: colorantes SYTO™ 80-85 de 50 μl cada uno

Método de detecciónFluorescente

Tipo de colorantePermeabilidad celular

Emisión545, 544, 560, 559, 582, 583 nm

Intervalo de longitud de onda de excitación531, 530, 541, 543, 567, 567 nm

Para utilizar con (equipo)Microscopio de fluorescencia

Línea de productosSYTO

Cantidad1 kit

Condiciones de envíoTemperatura ambiente

Volumen (métrico)50 μl

Tipo de etiquetaFluorescente

Tipo de productoKit de muestreador de atenuador de tinciones

Sub Cellular LocalizationÁcidos nucleicos

Unit SizeEach

Contenido y almacenamiento

Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.

Preguntas frecuentes

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (2)

Citations & References

Abstract

SYTO probes in the cytometry of tumor cell death.

Authors:Wlodkowic D, Skommer J, Darzynkiewicz Z,

Journal:Cytometry A

PubMed ID:18260152

Apoptosis is a complex and finely controlled cell death process of great relevance in tissue homeostasis and pathogenesis. The majority of classical apoptotic features can be examined by flow as well as image cytometry. Therefore, cytometry has been used as a technology of choice in studies of tumor cell demise. ... More

Synergistic effects of proteasome inhibitor carfilzomib in combination with tyrosine kinase inhibitors in imatinib-sensitive and -resistant chronic myeloid leukemia models.

Authors:

Journal:Oncogenesis

PubMed ID:24590311