SYTOX™ Orange Nucleic Acid Stain - 5 mM Solution in DMSO

Citations & References (41)

Invitrogen™

SYTOX™ Orange Nucleic Acid Stain - 5 mM Solution in DMSO

SYTOX Orange dye stains nucleic acids in cells with compromised membranes. This stain is useful as an indicator of cellRead more

Catalog Number	Quantity
S11368	250 μL

Catalog number S11368

Price (MXN)

Quantity:

250 μL

SYTOX Orange dye stains nucleic acids in cells with compromised membranes. This stain is useful as an indicator of cell death and is much brighter than propidium iodide (P-1304).

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

ColorOrange

Detection MethodFluorescence

Dye TypeCell-Permeant

Emission570 nm

Excitation Wavelength Range547 nm

For Use With (Equipment)Fluorescence Microscope

FormSolution

FormatTube(s)

Product LineSYTOX

Quantity250 μL

Shipping ConditionRoom Temperature

Volume (Metric)250 μL

Label TypeFluorescent Dye

Product TypeNucleic Acid Stain

SubCellular LocalizationNucleic Acids

Unit SizeEach

Contents & Storage

Store in freezer at -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

How do SYTO dyes bind to DNA?

The binding mode of SYTO nucleic acid stains is unknown. However, the behavior of these and related nucleic acid dyes suggests the following binding properties:

1.They appear to contact the solvent (suggested by sensitivity to salt, divalent cations, and in particular, SDS) and thus are likely to have contacts in the grooves.
2.All SYTO dyes appear to show some base selectivity and are thus likely to have minor groove contacts.
3.They can be removed from nucleic acid via ethanol precipitation; this characteristic is not shared by ethidium bromide and other intercalators. Likewise, the dyes are not removed from nucleic acid via butanol or chloroform extraction. These extraction methods do remove ethidium bromide from nucleic acid. 4. SYTO binding is not affected by nonionic detergents.
5. SYTO dyes are not quenched by BrdU, so they do not bind nucleic acids in precisely the same way as Hoechst 33342 and DAPI ((4′,6-diamidino-2-phenylindole).

SYBR Green I has shown little mutagenicity on frameshift indicator strains, indicating that it isn't likely to strongly intercalate.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (41)

Citations & References

Abstract

Nucleosomes are exposed at the cell surface in apoptosis.

Authors:Radic M, Marion T, Monestier M

Journal:J Immunol

PubMed ID:15153485

'Apoptotic cells are considered the source of DNA, histones, and nucleoprotein complexes that drive the production of autoantibodies in systemic lupus erythematosus. However, the role of apoptotic cells in the activation of the immune system is not clear. To explore interactions that may initiate or sustain the production of anti-nuclear ... More

Peroxisome proliferator-activated receptor gamma-mediated regulation of neural stem cell proliferation and differentiation.

Authors:Wada K, Nakajima A, Katayama K, Kudo C, Shibuya A, Kubota N, Terauchi Y, Tachibana M, Miyoshi H, Kamisaki Y, Mayumi T, Kadowaki T, Blumberg RS

Journal:J Biol Chem

PubMed ID:16524877

'Peroxisome proliferator-activated receptor gamma (PPARgamma) plays an important role in insulin sensitivity, tissue homeostasis, and regulating cellular functions. We found high-level expression of PPARgamma in embryo mouse brain and neural stem cells (NSCs), in contrast to extremely low levels in adult mouse brain. Here, we show that PPARgamma mediates the ... More

Diverse microglial motility behaviors during clearance of dead cells in hippocampal slices.

Authors:Petersen MA, Dailey ME

Journal:Glia

PubMed ID:15042586

'We used two-channel three-dimensional time-lapse fluorescence confocal imaging in live rat hippocampal slice cultures (1-7 days in vitro) to determine the motility behaviors of activated microglia as they engage dead and dying cells following traumatic brain tissue injury. Live microglia were labeled with a fluorescently conjugated lectin (IB(4)), and dead ... More

Probing the kinetics of SYTOX Orange stain binding to double-stranded DNA with implications for DNA analysis.

Authors:Yan X, Habbersett RC, Yoshida TM, Nolan JP, Jett JH, Marrone BL

Journal:Anal Chem

PubMed ID:15924389

'Rapid binding kinetics of SYTOX Orange stain with double-stranded DNA (dsDNA) was revealed on the DNA fragment sizing flow cytometer. We demonstrated for the first time that the dye molecules could be adsorbed onto the capillary surface and native DNA fragments can be dynamically stained while passing through the capillary. ... More

Droplet microfluidic technology for single-cell high-throughput screening.

Authors:Brouzes E, Medkova M, Savenelli N, Marran D, Twardowski M, Hutchison JB, Rothberg JM, Link DR, Perrimon N, Samuels ML,

Journal:Proc Natl Acad Sci U S A

PubMed ID:19617544

'We present a droplet-based microfluidic technology that enables high-throughput screening of single mammalian cells. This integrated platform allows for the encapsulation of single cells and reagents in independent aqueous microdroplets (1 pL to 10 nL volumes) dispersed in an immiscible carrier oil and enables the digital manipulation of these reactors ... More

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