SYTOX™ Deep Red Nucleic Acid Stain, for fixed/dead cells, 50 μL - FAQs

View additional product information for SYTOX™ Deep Red Nucleic Acid Stain, for fixed/dead cells - FAQs (S11381, S11380)

3 product FAQs found

Can I use SYTOX Deep Red Nucleic Acid Stain with a cell permeable nucleic acid stain for live/dead cell analysis?

Yes. For live cell staining, you may use any of the blue, green, or orange SYTO dyes or any of the blue fluorescent Hoechst stains.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use SYTOX Deep Red Nucleic Acid Stain for cell cycle analysis?

We do not recommend using SYTOX Deep Red Nucleic Acid Stain for cell cycle analysis. Instead, we recommend using Vybrant DyeCycle Ruby Stain (Cat Nos. V10273, V10309).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How is SYTOX Deep Red Nucleic Acid Stain, for fixed/dead cells (Cat. Nos. S11380, S11381) different from SYTOX Red Dead Cell Stain (Cat No. S34859)?

Although they are both far red, cell impermeant nucleic acid stains, there is approximately a 20 nm difference in the excitation and emission maxima: SYTOX Deep Red Nucleic Acid Stain ex/em = 660/682 nm and SYTOX Red Dead Cell Stain ex/em = 640/658. In addition, the main difference is that SYTOX Deep Red Nucleic Acid Stain has less propensity to stain RNA compared to SYTOX Red Dead Cell Stain.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.