Comodulation of cellular polyamines and proliferation: biomarker application to colorectal mucosa.
AuthorsHiguchi CM,Wang W
JournalJournal of cellular biochemistry
PubMed ID7759562
Polyamines are low molecular weight aliphatic amines required for normal cellular growth which are ubiquitously found in all living tissues. Polyamine biosynthesis is known to increase with mitogenesis, and elevated polyamine concentrations are found in hyperproliferative tissues. Quantitation of tissue polyamine content may thus provide a biochemical measure of proliferation, ... More
Human (MDR1) and mouse (mdr1, mdr3) P-glycoproteins can be distinguished by their respective drug resistance profiles and sensitivity to modulators.
AuthorsTang-Wai DF, Kajiji S, DiCapua F, de Graaf D, Roninson IB, Gros P
JournalBiochemistry
PubMed ID7819214
Possible functional differences between P-glycoproteins (P-gps) encoded by the human MDR1 and mouse mdr1 and mdr3 genes with respect to drug resistance profiles and sensitivity to known modulators have been investigated. For this, the three genes were introduced and overexpressed in the same cellular background, that of Chinese hamster LR73 ... More
Comparison of Hoechst 33342 and propidium iodide as fluorescent markers for sperm fusion with hamster oocytes.
AuthorsGreen DP
JournalJ Reprod Fertil
PubMed ID1285064
'Hamster oocytes were loaded with the DNA dyes Hoechst 33342 or propidium iodide. Oocytes incubated in 10 mumol Hoechst 333421(-1) showed intracellular fluorescence within 10-20 s of exposure, as did hamster and guinea-pig spermatozoa. Impaled oocytes to which acrosome-intact hamster spermatozoa were bound before injection of Hoechst 33342 showed dye ... More
Folic acid-enhanced synergy for the combination of trimetrexate plus the glycinamide ribonucleotide formyltransferase inhibitor 4-[2-(2-amino-4-oxo-4,6,7,8-tetrahydro-3H-pyrimidino[5,4,6][1,4]thiazin -6-yl)-(S)-ethyl]-2,5-thienoylamino-L-glutamic acid (AG2034): comparison across sensitive and resistant human tumor cell lines.
AuthorsFaessel HM, Slocum HK, Rustum YM, Greco WR
JournalBiochem Pharmacol
PubMed ID9952321
'Folic acid (PteGlu)-enhanced intense synergy has been observed between nonpolyglutamylatable dihydrofolate reductase (DHFR) inhibitors and polyglutamylatable inhibitors of other folate-requiring enzymes, such as glycinamide ribonucleotide formyltransferase (GARFT) and thymidylate synthase. Since this phenomenon is potentially therapeutically useful, we explored its universality by examining the combined action of a DHFR inhibitor, ... More
Cell-to-cell diffusion of fluorescent dyes in paired ventricular cells.
AuthorsImanaga I, Kameyama M, Irisawa H
JournalAm J Physiol
PubMed ID3812712
'The intracellular and cell-to-cell diffusion of fluorescent dyes of various molecular sizes were studied in enzymatically isolated paired ventricular cells of the guinea pig heart. Fluorescein sodium (mol wt 332), 6-carboxyfluorescein (mol wt 376), Lucifer yellow CH (mol wt 457), lissamine rhodamine B-200 (mol wt 559), and tetraglycine-conjugated lissamine rhodamine ... More
Forskolin-induced clearance of the fluorescent dye sulforhodamine from rat parotid intralobular duct lumen: visualization of the secretory function under a confocal laser scanning microscope.
AuthorsNakamoto T, Hirono C, Sugita M, Takemoto K, Iwasa Y, Akagawa Y, Shiba Y
JournalJ Membr Biol
PubMed ID12533784
'Cyclic AMP evokes fluid secretion with bicarbonate in exocrine ducts. Clearance of fluorescent dyes from rat parotid intralobular ducts by forskolin was visualized as a fluorescence change in the duct luminal space by optical sectioning under a confocal laser scanning microscope to clarify the secretory function in the ducts. When ... More
Isolation of a fluorophore-specific DNA aptamer with weak redox activity.
AuthorsWilson C, Szostak JW
JournalChem Biol
PubMed ID9831529
'BACKGROUND: In vitro selection experiments with pools of random-sequence nucleic acids have been used extensively to isolate molecules capable of binding specific ligands and catalyzing self-modification reactions. RESULTS: In vitro selection from a random pool of single-stranded DNAs has been used to isolate molecules capable of recognizing the fluorophore sulforhodamine ... More
Transferrin conjugates of doxorubicin: synthesis, characterization, cellular uptake, and in vitro efficacy.
AuthorsKratz F, Beyer U, Roth T, Tarasova N, Collery P, Lechenault F, Cazabat A, Schumacher P, Unger C, Falken U
JournalJ Pharm Sci
PubMed ID9523988
'One strategy for improving the antitumor selectivity and toxicity profile of antitumor agents is to design drug carrier systems employing suitable carrier proteins. Thus, thiolated human serum transferrin was conjugated with four maleimide derivatives of doxorubicin that differed in the stability of the chemical link between drug and spacer. Of ... More
Spectrophotometric determination of clonogenic capacity of leukemic cells in a semisolid microtiter culture system.
AuthorsSchweitzer CM, van de Loosdrecht AA, Jonkhoff AR, Ossenkoppele GJ, Huijgens PC, Dräger AM, Broekhoven MG, Langenhuijsen MM
JournalExp Hematol
PubMed ID8462666
'In this study we describe a semiautomated clonogenic assay in which human leukemic cell lines (U937 and HL60) are cultured in a semisolid 96-well microtiter culture system and clonogenicity is measured spectrophotometrically in a 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT)-assay and sulforhodamine (SRB)-assay. Culture medium with 0.6% (wt/vol) methylcellulose and 10% ... More
Isolation and characterization of fluorophore-binding RNA aptamers.
AuthorsHoleman LA, Robinson SL, Szostak JW, Wilson C
JournalFold Des
PubMed ID9889155
'BACKGROUND: In vitro selection has been shown previously to be a powerful method for isolating nucleic acids with specific ligand-binding functions (''aptamers''). Given this capacity, we have sought to isolate RNA motifs that can confer fluorescent labeling to tagged RNA transcripts, potentially allowing in vivo detection and in vitro spectroscopic ... More
Apoptosis as a measure of chemosensitivity to cisplatin and taxol therapy in ovarian cancer cell lines.
AuthorsGibb RK, Taylor DD, Wan T, O'Connor DM, Doering DL, Gerçel-Taylor C
JournalGynecol Oncol
PubMed ID9103385
'OBJECTIVE: Cisplatin- and Taxol-induced apoptosis was studied in four human ovarian cancer cell lines to evaluate apoptosis as a measure of chemosensitivity. METHODS: In vitro sensitivities of OVCAR-3, SKOV-3, UL-1, and UL-2 cells to cisplatin or Taxol were determined by the sulforhodamine B assay. Induction of apoptosis was studied by ... More
Effect of crosslinked poly(1-vinyl-2-pyrrolidinone) gels on cell growth in static cell cultures.
AuthorsHong Y, Chirila TV, Fitton JH, Ziegelaar BW, Constable IJ
JournalBiomed Mater Eng
PubMed ID9171901
'Poly(1-vinyl-2-pyrrolidinone) (PVP) and copolymers of 1-vinyl-2-pyrrolidinone are insoluble in water when crosslinked but they can absorb very large amounts of water to become syringe-injectable hydrogels. Such gels have been investigated recently as potential substitutes for the vitreous humour in the eye. In this study, during the cytotoxic evaluation by sulforhodamine ... More
Time-resolved total internal reflection fluorometry study on polarity at a liquid/liquid interface.
AuthorsIshizaka S, Kim HB, Kitamura N
JournalAnal Chem
PubMed ID11403281
'The polarity of a water/oil (oil: cyclohexane, carbon tetrachloride, toluene, chlorobenzene, o-dichlorobenzene, or 1,2-dichloroethane) interface was investigated by means of time-resolved total-internal-reflection (TIR) fluorescence spectroscopy of a polarity-sensitive probe: sulforhodamine B (SRB). In bulk solutions, the nonradiative decay rate constant of SRB increased with an increase in a solvent polarity ... More
Evidence for uptake of vital dye by activated rat peritoneal mast cells: an in vitro imaging study.
AuthorsWei JY, Go VL, Taché Y, Kruger L
JournalNeuroimage
PubMed ID9343581
'Uptake of material from surrounding medium by activated rat peritoneal mast cells (PMCs) was studied using in vitro peritoneal eluate cells, the vital fluorescent dye sulforhodamine B (SFRM-B), secretagogue compound 48/80, and an imaging technique. PMCs, which undergo different states of degranulation, are shown to possess the ability to take ... More
The combination of paclitaxel with cisplatin exhibits antagonism in vitro against human melanoma.
AuthorsMohith A, Photiou A, Retsas S
JournalAnticancer Drugs
PubMed ID8826619
'The in vitro cytotoxicity of paclitaxel and cisplatin, alone and in combination, was evaluated against the established human melanoma cell line, G361, with either 1 or 24 h asynchronous paclitaxel exposure using the sulforhodamine B assay. As single agent, the mean cisplatin concentration which inhibited G361 cell growth by 50% ... More
Modeling of the time-dependency of in vitro drug cytotoxicity and resistance.
'For potential clinical extrapolation of in vitro findings, it is of interest to relate the measured effect of an anticancer agent to concentration and exposure time. The Hill model (A. V. Hill, J. Physiol., 40: iv-vii, 1910) is commonly used to describe pharmacodynamic (PD) effects, including drug-induced growth inhibition of ... More
Multiple pathways of organic anion secretion in renal proximal tubule revealed by confocal microscopy.
AuthorsMasereeuw R, Russel FG, Miller DS
JournalAm J Physiol
PubMed ID8997391
'Previous studies with p-aminohippurate (PAH) and fluorescein (FL) have shown that cellular uptake and tubular secretion of organic anions is driven by indirect coupling to sodium. Here we used killifish proximal tubules and laser-scanning confocal microscopy to study the transport of a larger organic anion, fluorescein-methotrexate (FL-MTX, mol mass 923 ... More
Spectra and fluorescence lifetimes of lissamine rhodamine, tetramethylrhodamine isothiocyanate, texas red, and cyanine 3.18 fluorophores: influences of some environmental factors recorded with a confocal laser scanning microscope.
AuthorsBrismar H, Trepte O, Ulfhake B
JournalJ Histochem Cytochem
PubMed ID7608524
'We report on the spectra and fluorescence lifetimes of four commonly used fluorophores: lissamine rhodamine (LRSC); tetramethyl rhodamine isothiocyanate (TRITC); Texas Red; and cyanine 3.18 (Cy-3). Fluorescence lifetime recordings revealed that these spectrally overlapping fluorophores can be individually detected by their lifetimes, indicating that at least four fluorophores can be ... More
Aplysia cell adhesion molecules and serotonin regulate sensory cell-motor cell interactions during early stages of synapse formation in vitro.
AuthorsZhu H, Wu F, Schacher S
JournalJ Neurosci
PubMed ID7965085
'Long-term facilitation of sensorimotor synapses of Aplysia in culture by serotonin (5-HT) is accompanied by two changes: an increase in the number of sensory cell branches and varicosities contacting the major axons of the target motor cell L7, and a downregulation of Aplysia cell adhesion molecules (apCAM) from the surface ... More
The effects of pH and intraliposomal buffer strength on the rate of liposome content release and intracellular drug delivery.
AuthorsLee RJ, Wang S, Turk MJ, Low PS
JournalBiosci Rep
PubMed ID9743475
'Targeted liposomal drug formulations may enter cells by receptor-mediated endocytosis and then traffick by membrane flow into acidic intracellular compartments. In order to understand the impact of these intracellular pH changes on liposomal drug unloading, the effect of pH on the release from folate-targeted liposomes of three model compounds with ... More
Fluorescent taxoids.
AuthorsGuy R, Scott Z, Sloboda R, Nicolaou K
JournalChem Biol
PubMed ID9000007
'BACKGROUND: Taxol is a natural product produced by the Pacific Yew, Taxus brevifolia, that has emerged as a prominent chemotherapeutic agent for the treatment of solid tumors. Taxol''s biochemical mode of action has been well studied: it binds to microtubules, stabilizing them and preventing their depolymerization to tubulin subunits. At ... More
Lipophilic cations: a group of model substrates for the multidrug-resistance transporter.
AuthorsGros P, Talbot F, Tang-Wai D, Bibi E, Kaback HR
JournalBiochemistry
PubMed ID1371401
'The possibility that simple lipophilic cations such as tetraphenylphosphonium (TPA+), triphenylmethylphosphonium (TPMP+), and diphenyldimethylphosphonium (DDP+) are substrates for the multidrug-resistance transport protein, P-glycoprotein, was tested. Hamster cells transfected with and overexpressing mouse mdr1 or mouse mdr3 exhibit high levels of resistance to TPP+ and TPA+ (20-fold) and somewhat lower levels ... More
2-substituted 1,2-dihydro-3H-dibenz[de,h]isoquinoline-1,3-diones. A new class of antitumor agent.
AuthorsSami SM, Dorr RT, Alberts DS, Remers WA
JournalJ Med Chem
PubMed ID8459403
'A new class of antitumor agents, having structural analogy to amonafide, but differing by the addition of a fourth ring in the nucleus, was synthesized conveniently from anthracene. Compounds with a variety of substituents, containing a basic nitrogen atom and located on the imide nitrogen, were prepared. Thirteen of 19 ... More
Polyanionic agents as inhibitors of phagosome-lysosome fusion in cultured macrophages: evolution of an alternative interpretation.
AuthorsGoren MB, Vatter AE, Fiscus J
JournalJ Leukoc Biol
PubMed ID3468191
'Various natural and synthetic substances classified as polyanionics have been implicated in antagonizing phagosome-lysosome fusion in cultured macrophages. The phenomenon has been judged by comparing the transfer of selected markers from secondary lysosomes to phagosomes in control and in "polyanion" cells. Our earlier studies showed that use of one of ... More
Caries diagnosis with dye-staining at amalgam restoration margins.
AuthorsBoston DW, Cotmore JM, Sperrazza L
JournalAm J Dent
PubMed ID8695001
'PURPOSE: To determine the strength of agreement between detection of caries at the margin of amalgam restorations with a caries detector dye, and detection of caries histologically utilizing light/polarizing light microscopy. MATERIALS AND METHODS: 17 permanent molars with intact amalgam restorations, determined to be caries-free by visual inspection and explorer ... More
New colorimetric cytotoxicity assay for anticancer-drug screening.
'We have developed a rapid, sensitive, and inexpensive method for measuring the cellular protein content of adherent and suspension cultures in 96-well microtiter plates. The method is suitable for ordinary laboratory purposes and for very large-scale applications, such as the National Cancer Institute''s disease-oriented in vitro anticancer-drug discovery screen, which ... More
Novel homogeneous liposomal immunoassay for colorimetric estimation of serum IgG anticardiolipin antibodies.
AuthorsFrost SJ, Chakraborty J, Firth GB
JournalClin Chem
PubMed ID8665678
'Liposomes entrapping the dye sulforhodamine B were used to develop an assay for anticardiolipin antibodies (ACAs). In the presence of magnesium ions, IgG ACAs induced liposomal lysis and the resulting absorbance changes were dependent on the amount of ACAs present. The liposomal assay (y) showed similar intraassay imprecision and detection ... More
A strip liposome immunoassay for aflatoxin B1.
AuthorsHo JA, Wauchope RD
JournalAnal Chem
PubMed ID12033235
'A technique has been developed for the preparation of aflatoxin B1 (AFB1)-tagged liposomes encapsulating a visible dye. These liposomes have several useful potential analytical applications, one of which is demonstrated. A simple plastic-backed nitrocellulose strip is the basis for an assay for detecting AFB1. Samples containing aflatoxin B1 are allowed ... More
Clomiphene analogs with activity in vitro and in vivo against human breast cancer cells.
AuthorsBaumann RJ, Bush TL, Cross-Doersen DE, Cashman EA, Wright PS, Zwolshen JH, Davis GF, Matthews DP, Bender DM, Bitonti AJ
JournalBiochem Pharmacol
PubMed ID9586957
'Six hundred triphenylethylenes were assayed for antiproliferative activity against MCF-7, LY2, and MDA-MB-231 breast cancer cells using sulforhodamine B dye to measure proliferation. Here we report on just 63 of the compounds, mostly clomiphene analogs, with substitutions on the alpha'' or beta ring, at the vinyl position or in the ... More
Imaging of fluorescent molecule and small ion transport through human stratum corneum during high voltage pulsing: localized transport regions are involved.
'During the application of high-voltage pulses across the skin, transport of two negatively charged fluorescent molecules through the stratum corneum is highly localized. The apparent size of these localized transport regions (LTR''s) is initially 10 microns in diameter for both calcein and sulforhodamine. Appearance of LTR''s occurred at or above ... More
Stabilization of exocytosis by dynamic F-actin coating of zymogen granules in pancreatic acini.
AuthorsNemoto T, Kojima T, Oshima A, Bito H, Kasai H
JournalJ Biol Chem
PubMed ID15184362
'Reorganization of F-actin in the apical region of mouse pancreatic acinar cells during Ca(2+)-dependent exocytosis of zymogen granules was investigated by two-photon excitation microscopy with intact acini. Granules were rapidly coated with F-actin in response to either agonist stimulation or photolysis of a caged-Ca(2+) compound. Such F-actin coating occurred exclusively ... More
Unilamellar vesicle formation and encapsulation by microfluidic jetting.
AuthorsStachowiak JC, Richmond DL, Li TH, Liu AP, Parekh SH, Fletcher DA,
JournalProc Natl Acad Sci U S A
PubMed ID18353990
'Compartmentalization of biomolecules within lipid membranes is a fundamental requirement of living systems and an essential feature of many pharmaceutical therapies. However, applications of membrane-enclosed solutions of proteins, DNA, and other biologically active compounds have been limited by the difficulty of forming unilamellar vesicles with controlled contents in a repeatable ... More
Fluorescence imaging of light absorption for axial-beam geometry in capillary electrophoresis.
AuthorsJohansson J, Johansson T, Nilsson S
JournalElectrophoresis
PubMed ID9761209
'A new method for investigation of axial-beam absorption detection for improved detection limits in microcolumn separations is reported. The method is based on fluorescence imaging of light absorption along a separation capillary. The probing UV light is introduced at one end of the capillary and shows an exponential fall-off along ... More
Flow of water between aqueous and vitreous compartments in the rabbit eye.
AuthorsMaurice DM
JournalAm J Physiol
PubMed ID3812693
'When fluoresceinated dextran (FD) is injected into the vitreous body of the rabbit, it diffuses out of the eye through the anterior chamber so that the ratio of its concentration in the aqueous to that in the vitreous humor remains constant. The rate of loss of the substance from the ... More
Efficient fixation procedure of human leukemia cells in sulforhodamine B cytotoxicity assay.
AuthorsKim HM, Han SB, Kim MS, Kang JS, Oh GT, Hong DH
JournalJ Pharmacol Toxicol Methods
PubMed ID8959582
'The fixation procedures in sulforhodamine B (SRB) assay for human leukemia cells were modified to produce more reliable results. It was found that the concentration of the fixative agent, trichloroacetic acid (TCA), was critical in the selective fixation of cellular protein. While a TCA solution of 80% fixed both cells ... More
Zymogen granule exocytosis is characterized by long fusion pore openings and preservation of vesicle lipid identity.
AuthorsThorn P, Fogarty KE, Parker I
JournalProc Natl Acad Sci U S A
PubMed ID15090649
'The dynamics of the fusion pore that forms between a secretory vesicle and the plasma membrane are important in the regulation of both exocytosis and endocytosis. Here, we describe characteristics of fusion during zymogen granule exocytosis in exocrine pancreatic acinar cells. By using fluorescence recovery after photobleaching techniques, we show ... More
Potentiation of the antiviral activity of poly r(A-U) by xanthene dyes.
AuthorsJamison JM, Krabill K, Hatwalkar A, Jamison E, Tsai CC
JournalCell Biol Int Rep
PubMed ID1964628
'Ten xanthene dyes (XAN) are evaluated for their ability to potentiate the antiviral activity of poly r(A-U) using a human foreskin fibroblast-vesicular stomatitis virus bioassay in which the XAN is combined with 0.2 mM poly r(A-U) to produce a XAN/ribonucleotide ratio of 1/4. Four of the ten XANs tested in ... More
Sulforhodamine labeling of neural circuits engaged in motor pattern generation in the in vitro turtle brainstem-cerebellum.
AuthorsKeifer J, Vyas D, Houk JC
JournalJ Neurosci
PubMed ID1494952
'A fluorescent molecular probe was used in combination with a novel in vitro preparation to study spatial patterns of neural activity associated with motor pattern generation. The in vitro brainstem-cerebellum preparation takes advantage of the turtle's unusual resistance to anoxia to preserve the entire neural network that connects the cerebellum, ... More
Cucurbitacin E targets proliferating endothelia.
AuthorsDuncan MD, Duncan KL
JournalJ Surg Res
PubMed ID9202647
'Tumor vasculature offers a target for drugs directed against proliferating endothelia. We hypothesized that cucurbitacin E (CuE), an actin-disrupting agent, would preferentially inhibit proliferating vs. quiescent endothelia. Log-phase and confluent ECV304 and HUVEC human endothelial cells were exposed to 10-200 nM CuE for 1-96 hr, and toxicity was determined at ... More
A dual-probe fluorescence method to examine selective perturbations of membrane permeability by melittin.
AuthorsEl Jastimi R, Lafleur M
JournalBiospectroscopy
PubMed ID10380080
'A new fluorescence method has been developed to measure simultaneously and independently the release of fluorophores from two vesicle populations. Calcein and sulforhodamine B were used as a probe couple: the leakage of these probes from vesicles can be recorded independently since they can be excited simultaneously at 510 nm, ... More
Relative roles of gap junction channels and cytoplasm in cell-to-cell diffusion of fluorescent tracers.
AuthorsSafranyos RG, Caveney S, Miller JG, Petersen NO
JournalProc Natl Acad Sci U S A
PubMed ID3470791
'Intercellular (tissue) diffusion of molecules requires cytoplasmic diffusion and diffusion through gap junctional (or cell-to-cell) channels. The rates of tissue and cytoplasmic diffusion of fluorescent tracers, expressed as an effective diffusion coefficient, De, and a cytoplasmic diffusion coefficient, Dcyt, have been measured among the developing epidermal cells of a larval ... More
The rate of diffusion of fluorophores through the corneal epithelium and stroma.
AuthorsAraie M, Maurice D
JournalExp Eye Res
PubMed ID3556451
'The time taken to cross the rabbit corneal epithelium and stroma was estimated for fluorescein (F), carboxyfluorescein (CF), rhodamine B (RB), and sulforhodamine B (SRB). Paired corneas were mounted in vitro; one was intact and the dye solution was kept in continuous contact with its epithelial surface; the epithelium was ... More
Two-photon microscopic analysis of acetylcholine-induced mucus secretion in guinea pig nasal glands.
AuthorsOshima A, Kojima T, Dejima K, Hisa Y, Kasai H, Nemoto T
JournalCell Calcium
PubMed ID15755496
'The spatiotemporal changes in intracellular free Ca(2+) concentration ([Ca(2+)](i)) as well as fluid secretion and exocytosis induced by acetylcholine (ACh) in intact acini of guinea pig nasal glands were investigated by two-photon excitation imaging. Cross-sectional images of acini loaded with the fluorescent Ca(2+) indicator fura-2 revealed that the ACh-evoked increase ... More
Interaction between oleic acid-containing pH-sensitive and plain liposomes. Fluorescent spectroscopy studies.
AuthorsTorchilin VP, Lukyanov AN, Klibanov AL, Omelyanenko VG
JournalFEBS Lett
PubMed ID1299612
'The energy transfer method has been applied to study the interaction between pH-sensitive liposomes (phosphatidyl ethanolamine/oleic acid/cholesterol, 4:2:4 molar ratio) and plain liposomes (phosphatidyl choline/phosphatidyl ethanolamine/cholesterol, 4:2:3 molar ratio). It was shown that a slow fusion process occurs between two types of liposomes. Also, the transfer of oleic acid from ... More
Fluorescence methods for monitoring phagosome-lysosome fusion in human macrophages.
AuthorsDüzgünes N, Majumdar S, Goren MB
JournalMethods Enzymol
PubMed ID8361377
Cytotoxic effect of vesicles produced by Porphyromonas gingivalis on fibroblasts in culture.
AuthorsGuigand M, Pellen P, Mouton C, Bonnaure-Mallet M
JournalJ Periodontal Res
PubMed ID7776155
It has been shown that the vesicles produced by Porphyromonas gingivalis under certain growth conditions contribute to its pathogenicity. In this study, we demonstrate the cytotoxic effect of the vesicles using two methods: one quantitative (the colorimetric cytotoxicity test using sulforhodamine B) and the other qualitative (flow cytometry). ... More
Boron trifluoride etherate on silica-A modified Lewis acid reagent (VII). Antitumor activity of cannabigerol against human oral epitheloid carcinoma cells.
AuthorsBaek SH, Kim YO, Kwag JS, Choi KE, Jung WY, Han DS
JournalArch Pharm Res
PubMed ID9875457
Geraniol (1), olivetol (2), cannabinoids (3 and 4) and 5-fluorouracil (5) were tested for their growth inhibitory effects against human oral epitheloid carcinoma cell lines (KB) and NIH 3T3 fibroblasts using two different 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and sulforhodamine B protein (SRB) assay. Cannabigerol (3) exhibited the highest growth-inhibitory activity ... More
Comparison of the sulforhodamine B protein and tetrazolium (MTT) assays for in vitro chemosensitivity testing.
The sulforhodamine B (SRB) protein stain assay was compared with the tetrazolium (MTT) colorimetric assay for in vitro chemosensitivity testing of various human tumour cell lines. The SRB assay provided a better linearity with cell number and a higher sensitivity, and its staining was not cell-line dependent. In contrast to ... More
[Histotopochemical investigations on elastic membranes of blood vessels with special regard to diabetes mellitus. III. Lipids (author's transl)]
AuthorsOpitz M, von Dorsche HH
JournalActa Histochem
PubMed ID818868
Lipids have been investigated in the light microscope with Scarlet-Blue and for the fluorescentmicroscopy with rhodamine B and sulforhodamine B. Both of these reactions gave weak results. After using nilblue-sulfate for the detecting of acid and neutral lipids the presence of acid lipids could be shown in the walls of ... More
A permeability test for acute corneal toxicity.
AuthorsMaurice D, Singh T
JournalToxicol Lett
PubMed ID3715923
The penetration of sulforhodamine B (SRB) into the two corneas of a freshly killed mouse was measured after one eye was briefly exposed to a solution of a possibly toxic substance. The ratio of the penetrations provides a numerical index of acute toxicity to the corneal epithelium. With a group ... More
Immobilon-P transfer membrane: applications and utility in protein biochemical analysis.
AuthorsLeGendre N
JournalBiotechniques
PubMed ID2271178
Polyvinylidene fluoride membrane for protein-blotting applications was introduced by Millipore Corporation in 1986. The membrane, designated variously as Immobilon-PVDF, Immobilon-P Transfer Membrane and Immobilon-P, has been widely used in protein biochemical analysis. Applications developed using Millipore's polyvinylidene fluoride membrane span immunochemistry, cell biology and protein structural characterization. Additionally, protein analysis ... More
Fluorescent markers for studying phagosome-lysosome fusion.
AuthorsGoren MB, Swendsen CL, Fiscus J, Miranti C
JournalJ Leukoc Biol
PubMed ID6384402
Lysosomotropic fluorescent aminoacridines such as acridine orange and quinacrine have achieved prominence as markers for studying lysosome-phagosomes fusion, especially in macrophages. Experiments described demonstrate that because the aminoacridines traverse biological membranes with facility, they diffuse throughout the system, and ultimately accumulate intra- or extracellularly where they are most efficiently bound. ... More
Comparison of two colorimetric assays as cytotoxicity endpoints for an in vitro screen for antitumour agents.
AuthorsFricker SP, Buckley RG
JournalAnticancer Res
PubMed ID9042253
Two colorimetric assays, MTT and SRB, were compared as cytotoxicity endpoints. They were evaluated against four anti-cancer drugs and seven human tumour cell lines, results were analyzed by ANOVA. Both assays gave a linear response with respect to cell number. The cell lines exhibited differential cytotoxicity to the cancer drugs. ... More
A highly sensitive immunoassay system involving antibody-coated tubes and liposome-entrapped dye.
AuthorsO'Connell JP, Campbell RL, Fleming BM, Mercolino TJ, Johnson MD, McLaurin DA
JournalClin Chem
PubMed ID4028391
In this colorimetric immunoassay for digoxin, large, unilamellar phospholipid vesicles approximately 0.2 micron in diameter are loaded with high concentrations of Sulforhodamine B. Digoxigenin coupled to phosphatidylethanolamine, incorporated into the lipid formulation, confers immunological specificity. The liposomes are then used as tracers in simple competitive-binding immunoassays with antibody-coated tubes. Results ... More
Single-molecule identification by spectrally and time-resolved fluorescence detection
AuthorsPrummer M, Hubner CG, Sick B, Hecht B, Renn A, Wild UP
JournalAnal Chem
PubMed ID10695126
A method to identify single molecules rapidly and with high efficiency based on simple probability considerations is proposed. In principle, any property of a detected photon in a single-molecule fluorescence experiment, e.g., emission wavelength, arrival time after pulsed excitation, and polarization, can be analyzed within the framework of the outlined ... More
Rapid staining of proteins on polyacrylamide gels and nitrocellulose membranes using a mixture of fluorescent dyes.
AuthorsGanesh G, Kumar TK, Pandian ST, Yu C
JournalJ Biochem Biophys Methods
PubMed ID11086192
The present work describes a novel, fluorescence-based method for staining proteins on SDS-PAGE and membrane(s). In this method, proteins are stained using a mixed-dye (sulfo-rhodamine B and 1-anilino-8-naphthalene sulfonic acid (NH(4)(+))) solution. The mixed-dye staining protocol can detect proteins up to a concentration of 15 ng. This method is generally ... More
Calcium buffering capacity of neuronal cell cytosol measured by flash photolysis of calcium buffer NP-EGTA.
AuthorsFleet A, Ellis-Davies G, Bolsover S
JournalBiochem Biophys Res Commun
PubMed ID9784424
N1E-115 mouse neuroblastoma cells were injected with a calcium buffer/indicator solution to allow both ratiometric measurement of free calcium concentration and the release of calcium ions upon UV flash. The solution contained sulforhodamine, a marker dye used to estimate the volume injected; fluo-3, a calcium indicator, and NP-EGTA, a high ... More
Differential and sequential delivery of fluorescent lysosomal probes into phagosomes in mouse peritoneal macrophages.
AuthorsWang YL, Goren MB
JournalJ Cell Biol
PubMed ID2438290
It has previously been inferred that the fusion of a macrophage secondary lysosome with a phagosome delivers the entire lysosomal contents uniformly to the phagosome. We found, however, that different fluorescent lysosomal probes can enter phagosomes at remarkably different rates, even though they are initially sequestered together in the same ... More
AuthorsRouach N, Koulakoff A, Abudara V, Willecke K, Giaume C,
JournalScience
PubMed ID19056987
Astrocytes provide metabolic substrates to neurons in an activity-dependent manner. However, the molecular mechanisms involved in this function, as well as its role in synaptic transmission, remain unclear. Here, we show that the gap-junction subunit proteins connexin 43 and 30 allow intercellular trafficking of glucose and its metabolites through astroglial ... More
Comparative in vitro sensitivity of human cholangiocarcinoma and colon adenocarcinoma cells to anticancer agents.
AuthorsVilla R, Gornati D, Zaffaroni N, Veneroni S, Bidoli P, Severini A
JournalAnticancer Res
PubMed ID9137435
We comparatively investigated the sensitivity of a human cholangiocarcinoma cell line (SG231) and an adenocarcinoma cell line (WiDr) to mitoxantrone (MX), taxol (TX), mitomycin C (MMC), doxorubicin (DX), cisplatin (CDDP) and 5-fluorouracil (5FU) by the sulforhodamine B assay. The lower susceptibility of SG231 to SFU, to CDDP, to DX and ... More
In vivo imaging of elastic fibers using sulforhodamine B.
AuthorsRicard C, Vial JC, Douady J, van der Sanden B,
JournalJ Biomed Opt
PubMed ID18163833
Until now, the imaging of elastic fibers was restricted to tissue sections using the endofluorescence properties of elastin or histological dyes. Methods to study their morphology in vivo and in situ have been lacking. We present and characterize a new application of a fluorescent dye for two-photon microscopy: sulforhodamine B ... More
A virocidal amphipathic {alpha}-helical peptide that inhibits hepatitis C virus infection in vitro.
An amphipathic alpha-helical peptide (C5A) derived from the membrane anchor domain of the hepatitis C virus (HCV) NS5A protein is virocidal for HCV at submicromolar concentrations in vitro. C5A prevents de novo HCV infection and suppresses ongoing infection by inactivating both extra- and intracellular infectious particles, and it is nontoxic ... More
ATP-induced P2X7-associated uptake of large molecules involves distinct mechanisms for cations and anions in macrophages.
AuthorsSchachter J, Motta AP, de Souza Zamorano A, da Silva-Souza HA, Guimarães MZ, Persechini PM,
JournalJ Cell Sci
PubMed ID18782864
Macrophages express the P2X(7) receptor and other nucleotide (P2) receptors, and display the phenomenon of extracellular ATP (ATP(e))-induced P2X(7)-dependent membrane permeabilization, which occurs through a poorly understood mechanism. We used patch-clamp recordings, cytoplasmic Ca(2+) measurements and fluorescent dye uptake assays to compare P2X(7)-associated transport phenomena of macrophages and HEK-293 cells ... More
Skin electroporation: rapid measurements of the transdermal voltage and flux of four fluorescent molecules show a transition to large fluxes near 50 V.
AuthorsChen T, Segall EM, Langer R, Weaver JC
JournalJ Pharm Sci
PubMed ID9811492
Large molecular fluxes and tight control are highly desired for transdermal drug delivery, which motivated this study of molecular transport due to high voltage pulsing. We used a flow-through sampling system (time resolution of approximately 14 s) to measure the response of human skin in vitro to a series of ... More
Evaluation of boron neutron capture effects in cell culture using sulforhodamine-B assay and a colony assay.
AuthorsWittig A, Sauerwein W, Pöller F, Fuhrmann C, Hideghéty K, Streffer C
JournalInt J Radiat Biol
PubMed ID9690686
PURPOSE: The purpose of this study was to find an in vitro method for determining the cytotoxicity of boronated drugs as well as their potential suitability for neutron capture therapy. MATERIALS AND METHODS: The survival of human melanoma cells has been determined by a colony assay and the sulforhodamine-B assay ... More
Target-dependent morphological segregation of Aplysia sensory outgrowth in vitro.
AuthorsGlanzman DL, Kandel ER, Schacher S
JournalNeuron
PubMed ID1764244
The adult nervous system is characterized by partial or complete morphological segregation of terminals from different afferent neurons innervating the same postsynaptic target. This segregation is thought to result, in part, from competition between the afferent terminals. To explore the role of the target cell in the spatial distribution of ... More
Comparison of sulforhodamine B, tetrazolium and clonogenic assays for in vitro radiosensitivity testing in human ovarian cell lines.
AuthorsGriffon G, Merlin JL, Marchal C
JournalAnticancer Drugs
PubMed ID7538827
The radiation sensitivity of six human ovarian tumor cell lines was evaluated using sulforhodamine B (SRB), tetrazolium (MTT) and clonogenic assays. Radiobiological parameters calculated from a linear quadratic model (SF2, alpha, beta) as well as from a single-hit multitarget model (D(o), Dq, n) and from the area under the dose-response ... More
Characteristics of DNA-tagged liposomes allowing their use in capillary-migration, sandwich-hybridization assays.
AuthorsRule GS, Montagna RA, Durst RA
JournalAnal Biochem
PubMed ID9025942
Liposomes that have been labeled externally with a DNA oligomer are used in a capillary-migration, sandwich-hybridization assay for specific DNA target sequences. The liposomes are used in a DNA detection scheme that produces visually observable results in 10 min. The preparation and covalent attachment of a thiol-activated 22-base oligomer to ... More
Nanoparticle assembly and transport at liquid-liquid interfaces.
AuthorsLin Y, Skaff H, Emrick T, Dinsmore AD, Russell TP
JournalScience
PubMed ID12522244
The self-assembly of particles at fluid interfaces, driven by the reduction in interfacial energy, is well established. However, for nanoscopic particles, thermal fluctuations compete with interfacial energy and give rise to a particle-size-dependent self-assembly. Ligand-stabilized nanoparticles assembled into three-dimensional constructs at fluid-fluid interfaces, where the properties unique to the nanoparticles ... More
A vesicle capture sensor chip for kinetic analysis of interactions with membrane-bound receptors.
AuthorsCooper MA, Hansson A, Löfås S, Williams DH
JournalAnal Biochem
PubMed ID10625506
A novel sensor chip for use in surface plasmon resonance (SPR) biosensors has been developed to capture vesicles which may contain membrane-bound receptors. Sulforhodamine-containing vesicles were shown by fluorescence microscopy to be immobilized intact on the sensor chip. Binding of cholera toxin to captured vesicles containing ganglioside GM(1) was demonstrated ... More
Optimization of the sulforhodamine B colorimetric assay.
AuthorsPapazisis KT, Geromichalos GD, Dimitriadis KA, Kortsaris AH
JournalJ Immunol Methods
PubMed ID9433470
Sulforhodamine B (SRB) protein staining has been widely used for cell proliferation and chemosensitivity testing, substituting for tetrazolium-based assays. However, the cell fixation step in the original assay is subject to error. We tested whether aspiration of medium with an automatic microplate multiwash device prior to fixation improves the method ... More
Development of an in vitro hepatotoxicity assay for assessing the effects of chronic drug exposure.
AuthorsHall TJ, James PR, Cambridge G
JournalRes Commun Chem Pathol Pharmacol
PubMed ID8451546
We have used the human hepatoma cell line HepG2 to compare the hepatotoxic effects of acute (24 hr) and chronic (up to 10 days) exposure to amitriptyline, paracetamol and ondansetron. In acute exposure studies, hepatotoxicity was assessed by the sulforhodamine B protein staining method, where amitriptyline and paracetamol produced 50% ... More
A comparison of clonogenic, microtetrazolium and sulforhodamine B assays for determination of cisplatin cytotoxicity in human ovarian carcinoma cell lines.
AuthorsPerez RP, Godwin AK, Handel LM, Hamilton TC
JournalEur J Cancer
PubMed ID8398340
An assay based upon quantitative staining of cellular protein by sulforhodamine B (SRB) has recently been adopted by the NCI for large-scale screening of new drugs. However, there are few data available regarding whether the SRB assay is comparable to other established methods. Cisplatin cytotoxicity was determined in 16 human ... More
Two-photon excitation imaging of pancreatic islets with various fluorescent probes.
AuthorsTakahashi N, Nemoto T, Kimura R, Tachikawa A, Miwa A, Okado H, Miyashita Y, Iino M, Kadowaki T, Kasai H
JournalDiabetes
PubMed ID11815453
Various fluorescent probes were assessed for investigating intact islets of Langerhans using two-photon excitation imaging. Polar fluorescent tracers applied on the outside rapidly (within 3 min) penetrated deep into the islets via microvessels. Likewise, an adenovirus carrying a Ca(2+)-sensitive green fluorescent protein mutant gene, yellow cameleon 2.1, was successfully transfected ... More
Colorimetric chemosensitivity testing using sulforhodamine B.
AuthorsKubota T, Takahara T, Nagata M, Furukawa T, Kase S, Tanino H, Ishibiki K, Kitajima M
JournalJ Surg Oncol
PubMed ID8468977
A colorimetric chemosensitivity test was investigated using sulforhodamine B (SRB), which stains protein synthesized by cells, as an end-point marker. Four cultured cell lines, 9 human tumor xenografts serially transplanted into nude mice, and 14 fresh surgical specimens were subjected to this assay. The optimal conditions for the assay were ... More
Modification of cell cycle and viability of TLX5 lymphoma in vitro by sulfoxide-ruthenium compounds and cisplatin detected by flow cytometry.
AuthorsCapozzi I, Clerici K, Cocchietto M, Salerno G, Bergamo A, Sava G
JournalChem Biol Interact
PubMed ID9630847
The effects of Na[trans-RuCl4(DMSO)Im] (NAMI), Na[trans-RuCl4(TMSO) Ind] (TIND) and Na[trans-RuCl4(TMSO)Iq] TEQU) were tested in vitro on TLX5 lymphoma cells in comparison to cisplatin by means of the sulforhodamine-B test SRB) for protein content determination, by acridine orange and propidium iodide staining and by means of the bromodeoxyuridine test, for cell ... More
Liposome dehydration on nitrocellulose and its application in a biotin immunoassay.
AuthorsMartorell D, Siebert ST, Durst RA
JournalAnal Biochem
PubMed ID10419633
The feasibility of utilizing dehydrated liposomes in the development of a simple immunoassay device for point-of-care diagnostics or field assays was demonstrated. The recovery of liposomes after a cycle of dehydration and rehydration was studied using biotin-tagged, dye-loaded liposomes with antibiotin antibodies immobilized in a defined zone on nitrocellulose strips. ... More
Size selectivity between gap junction channels composed of different connexins.
AuthorsGong XQ, Nicholson BJ
JournalCell Commun Adhes
PubMed ID12064586
Gap junction channels are traditionally viewed as large, nonspecific pores connecting cells. Recently the diversity in the connexin family has drawn more attention to their permeability characteristics. Several studies have shown that both size and charge contribute to the permeability of gap junctional channels. We have used a graded series ... More
Two phases of zymogen granule lifetime in mouse pancreas: ghost granules linger after exocytosis of contents.
AuthorsThorn P, Parker I
JournalJ Physiol
PubMed ID15637100
Different cell types show widely divergent mechanisms and kinetics of exocytosis. We investigated these processes in pancreatic acinar cells by using video-rate 2-photon microscopy to image entry of extracellular dye into individual zymogen granules undergoing exocytosis. Fluorescence signals display two distinct phases; an initial peak that then decays over several ... More
Immunoliposome sandwich assay for the detection of Escherichia coli O157:H7.
AuthorsPark S, Durst RA
JournalAnal Biochem
PubMed ID10805533
We describe the development of a field-portable colorimetric immunoassay for the detection of Escherichia coli O157:H7, using antibody-directed liposomes (immunoliposomes) encapsulating dye as an analytical reagent. Antibodies (anti-E. coli O157:H7) thiolated by 2-iminothiolane were coupled to malemide-tagged liposomes encapsulating the marker dye, sulforhodamine B. Transmission electron microscopy showed that the ... More
Excision repair cross-complementing rodent repair deficiency gene 2 expression and chloroethylnitrosourea resistance in human glioma cell lines.
AuthorsChen ZP, McQuillan A, Mohr G, Panasci LC
JournalNeurosurgery
PubMed ID9588557
OBJECTIVE: Nitrosoureas are the standard chemotherapeutic agents for malignant brain tumors. However, their anticancer effects are limited because many tumors are resistant to these agents. Nucleotide excision repair can repair bulky deoxyribonucleic acid adducts, including deoxyribonucleic acid damage induced by ultraviolet light and some chemotherapeutic agents, and may be implicated ... More
Circumvention of P-glycoprotein-mediated multiple drug resistance by phosphorylation modulators is independent of protein kinases.
AuthorsSmith CD, Zilfou JT
JournalJ Biol Chem
PubMed ID7499304
Expression of P-glycoprotein by tumor cells confers resistance to multiple natural product drugs because of its ability to export these compounds. This transporter is a substrate for several protein kinases; however, the functional significance of its phosphorylation is not defined. We examined the effects of many activators and inhibitors of ... More
Identification of myocardial proteins from two-dimensional gels by peptide mass fingerprinting.
AuthorsSutton CW, Pemberton KS, Cottrell JS, Corbett JM, Wheeler CH, Dunn MJ, Pappin DJ
JournalElectrophoresis
PubMed ID7607162
Two-dimensional gels offer a powerful method for separating complex protein mixtures, but subsequent methods for analysing individual components, such as protein sequencing and Western immunoblotting, are laborious and slow. The identification of proteins can be accelerated by using a combination of protease digestion and matrix assisted laser desorption-mass spectrometry (MALDI-MS). ... More
Effects of melatonin on proliferation of cancer cell lines.
AuthorsPapazisis KT, Kouretas D, Geromichalos GD, Sivridis E, Tsekreli OK, Dimitriadis KA, Kortsaris AH
JournalJ Pineal Res
PubMed ID9885990
The pineal hormone melatonin has been reported to have in vitro antiproliferative effects on estrogen receptor-positive human breast cancer cell lines at concentrations near to plasma physiological concentrations (1 x 10(-11) to 1 x 10(-9) M). Its growth inhibitory actions have been thought to be linked to the estrogen-receptor system. ... More
Antimony trichloride induces DNA damage and apoptosis in mammalian cells.
AuthorsHuang H, Shu SC, Shih JH, Kuo CJ, Chiu ID
JournalToxicology
PubMed ID9772090
Antimony compounds are widely used in various manufacturing and semiconducting industries. Previously, it has been shown that antimony trichloride (SbCl3) elevates sister chromatid exchange (SCE) rates in V79 cells after a 28-h incubation. However, only limited data on its genotoxic effects are available so far. The present results demonstrate that ... More
Schedule-dependent interaction of doxorubicin, paclitaxel and gemcitabine in human breast cancer cell lines.
AuthorsZoli W, Ricotti L, Barzanti F, Dal Susino M, Frassineti GL, Milandri C, Casadei Giunchi D, Amadori D
JournalInt J Cancer
PubMed ID9935183
We showed previously that a sequential treatment with doxorubicin (4 hr) followed by paclitaxel (24 hr) (Dox-->Pacl) induces a synergistic cytotoxic effect in the BRC-230 breast cancer cell line and in human primary breast cancer cultures. The validity of this experimental finding was confirmed in a clinical phase I/II study ... More
Comparison of in vitro anticancer-drug-screening data generated with a tetrazolium assay versus a protein assay against a diverse panel of human tumor cell lines.
AuthorsRubinstein LV, Shoemaker RH, Paull KD, Simon RM, Tosini S, Skehan P, Scudiero DA, Monks A, Boyd MR
JournalJ Natl Cancer Inst
PubMed ID2359137
The National Cancer Institute (NCI) is implementing a large-scale in vitro drug-screening program that requires a very efficient automated assay of drug effects on tumor cell viability or growth. Many laboratories worldwide have adopted a microculture assay based on metabolic reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). However, because of certain technical ... More
A monoclonal antibody-beta-glucuronidase conjugate as activator of the prodrug epirubicin-glucuronide for specific treatment of cancer.
AuthorsHaisma HJ, Boven E, van Muijen M, de Jong J, van der Vijgh WJ, Pinedo HM
JournalBr J Cancer
PubMed ID1520585
The anti-pan carcinoma monoclonal antibody (MAb) 323/A3, linked to E. coli-derived beta-glucuronidase (GUS) was used to study the tumour-site-selective activation of the prodrug Epirubicin-glucuronide (Epi-glu). Epi-glu was isolated from the urine of patients treated with Epirubicin (Epi) by reversed phase chromatography on a silica-C18 column. Epi-glu was stable in human ... More
Measurement of transmission of ultraviolet and visible light in the living rabbit cornea.
AuthorsMcLaren JW, Brubaker RF
JournalCurr Eye Res
PubMed ID8670741
Corneal transmittance in pigmented rabbits was measured at ultraviolet and visible wavelengths from the ratio of fluorescence of dyes in the anterior chamber to fluorescence of the same dyes in a quartz cuvette. Aqueous humor was drained through a limbal incision and the anterior chamber was reformed with a mixture ... More
Double fluorescent vital assay of phagocytosis by cultured retinal pigment epithelial cells.
AuthorsMcLaren MJ, Inana G, Li CY
JournalInvest Ophthalmol Vis Sci
PubMed ID7680023
PURPOSE. The goal of this study was to develop the first vital assay system for in vitro analysis of phagocytosis of rod outer segments (ROS) by normal retinal pigment epithelial (RPE) cells and for study of the phagocytic defect in RPE of the Royal College of Surgeons (RCS) rat with ... More
Interaction of liposomes with human skin in vitro--the influence of lipid composition and structure.
AuthorsKirjavainen M, Urtti A, Jääskeläinen I, Suhonen TM, Paronen P, Valjakka-Koskela R, Kiesvaara J, Mönkkönen J
JournalBiochim Biophys Acta
PubMed ID8982264
Liposomes have been suggested as a vehicle for dermal and transdermal drug delivery, but the knowledge about the interaction between lipid vesicles and human skin is poor. Therefore, we visualized liposome penetration into the human skin by confocal laser scanning microscopy (CLSM) in vitro. Liposomes were prepared from phospholipids in ... More
Using the national cancer institute anticancer drug screen to assess the effect of MRP expression on drug sensitivity profiles.
AuthorsAlvarez M, Robey R, Sandor V, Nishiyama K, Matsumoto Y, Paull K, Bates S, Fojo T
JournalMol Pharmacol
PubMed ID9804615
The MRP gene contributes to one form of multidrug resistance. To identify drugs interacting with MRP, we measured MRP mRNA expression by quantitative PCR in 60 cell lines of the National Cancer Institute Anticancer Drug Screen. Expression was detected in all cell lines (highest in lung carcinomas and central nervous ... More
Effect of gonadotropin-releasing hormone analogs and their conjugates on gonadotropin-releasing hormone receptor--positive human cancer cell lines.
AuthorsPályi I, Vincze B, Kálnay A, Turi G, Mezõ I, Teplán I, Seprõdi J, Pató J, Móra M
JournalCancer Detect Prev
PubMed ID8706040
The purpose of the present investigation was to develop new gonadotropin-releasing hormone (GnRH) antagonists and to increase their stability and antitumor effect by conjugation with carrier macromolecules. Antitumor effect was evaluated using clonogenic assay, cell counting for antiproliferation, and sulforhodamine B method. The presence of GnRH-binding sites in human cancer ... More
Poly(L-lactic acid) foams with cell seeding and controlled-release capacity.
AuthorsLo H, Kadiyala S, Guggino SE, Leong KW
JournalJ Biomed Mater Res
PubMed ID8847355
A synthetic porous three-dimensional structure that can mimic the architecture of actual tissues, provide sustained release of nutrients or growth factors, and serve as a template for cell seeding would be an ideal substrate for tissue engineering. Poly(l-lactic acid) (PLLA) foams were fabricated for this purpose, based on the principle ... More
Functional nucleoside transporters are required for gemcitabine influx and manifestation of toxicity in cancer cell lines.
AuthorsMackey JR, Mani RS, Selner M, Mowles D, Young JD, Belt JA, Crawford CR, Cass CE
JournalCancer Res
PubMed ID9766663
Gemcitabine (2',2'-difluorodeoxycytidine) is a novel pyrimidine nucleoside drug with clinical efficacy in several common epithelial cancers. We have proposed that gemcitabine requires nucleoside transporter (NT) proteins to permeate the plasma membrane and to exhibit pharmacological activity. In humans, there are seven reported distinct NT activities varying in substrate specificity, sodium ... More
Segregation of presynaptic inputs on an identified target neuron in vitro: structural remodeling visualized over time.
AuthorsBank M, Schacher S
JournalJ Neurosci
PubMed ID1494941
Sensory cells of Aplysia form chemical synapses with the motor cell L7 in culture. Under certain conditions, sensory cells will also form electrical connections with each other. Sites of chemical synaptic interaction between the sensory cells and L7 are located at varicosities along sensory cell processes that overlie the main ... More
Measurement of material extravasation in microvascular networks using fluorescence video-microscopy.
We have developed a new method using fluorescence videomicroscopy to quantitate the extravasation of intravenously injected materials. This method can measure the relative plasma concentration of, and the vascular permeability to, these materials in microcirculatory preparations which contain multiple blood vessels in a field of view. The image of a ... More
Differential inhibitory effects on human endometrial carcinoma cell growth of luteinizing hormone-releasing hormone analogues.
AuthorsBorri P, Coronnello M, Noci I, Pesciullesi A, Peri A, Caligiani R, Maggi M, Torricelli F, Scarselli G, Chieffi O, Mazzei T, Mini E
JournalGynecol Oncol
PubMed ID9887238
In addition to its function as a key hormone in the regulation of the pituitary-gonadal axis, luteinizing hormone-releasing hormone (LHRH) probably also affects various extrapituitary tissues. LHRH binding sites and in vitro antiproliferative effects of LHRH analogues have been reported in human endometrial cancer. The effects of the LHRH agonist ... More
Comparative in vitro cytotoxicity of taxol and Taxotere against cisplatin-sensitive and -resistant human ovarian carcinoma cell lines.
AuthorsKelland LR, Abel G
JournalCancer Chemother Pharmacol
PubMed ID1356649
Using the sulforhodamine B assay, we compared the cytotoxic properties of the novel microtubule agent taxol and the semi-synthetic related compound Taxotere in nine human ovarian-carcinoma cell lines, including three pairs of cell lines rendered resistant to cisplatin or carboplatin. In addition, the cytotoxicity of the commonly used anticancer drugs ... More
Expression of bcl-2 and bax in TGF-beta 1-induced apoptosis of L1210 leukemic cells.
TGF-beta1 is a multifunctional regulatory peptide (25 kDa) inducing growth arrest and apoptosis in many normal and neoplastic cells. In the present study, the involvement of proapoptotic (bax) and antiapoptotic (bcl-2) genes in the molecular mechanism of TGF-beta1-induced apoptosis of L1210 leukemic cells was investigated. Bax transcript was measured using ... More