Succinimidil 3-(2-piridilditio)propionato (SPDP)
Succinimidil 3-(2-piridilditio)propionato (SPDP)
Citas y referencias (163)
Invitrogen™

Succinimidil 3-(2-piridilditio)propionato (SPDP)

El entrecruzador heterobifuncional succinimidilo 3-(2-piridilditio) propionato (SPDP) permite vincular covalentemente dos grupos objetivo. El éster de succinidilo reacciona químicamente conMás información
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Número de catálogoCantidad
S1531100 mg
Número de catálogo S1531
Precio (MXN)
-
Cantidad:
100 mg
El entrecruzador heterobifuncional succinimidilo 3-(2-piridilditio) propionato (SPDP) permite vincular covalentemente dos grupos objetivo. El éster de succinidilo reacciona químicamente con aminas primarias y después de una reducción leve del ditiol, puede reaccionar formando un disulfuro con otros tioles. Los reactivos de entrecruzamiento son útiles para sondear las relaciones espaciales y las interacciones dentro y entre biomoléculas.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Permeabilidad celularSí
DescripciónSPDP
FormularioSólido
Método de etiquetadoEtiqueta química
Peso molecular312.36
PegiladoNo
Cantidad100 mg
Fracción reactivaÉster NHS, piridildisulfuro
SolubilidadDMF, DMSO
Longitud del brazo del separador6,8 Å
Soluble en aguaNo
Reactividad químicaAmino-sulfhidrilo
CleavablePor tioles
Tipo de enlace cruzadoHeterobifuncionales
FormatoEstándar
Tipo de productoEntrecruzador
SeparadorCorto (< 10 Å)
Unit SizeEach
Contenido y almacenamiento
Tras su recepción, se debe almacenar a -20 °C, protegido de la luz.

Preguntas frecuentes

Can you provide the shelf-life for Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)?

Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (163)

Citations & References
Abstract
Blockade of the galactose-binding sites of ricin by its linkage to antibody. Specific cytotoxic effects of the conjugates.
Authors:Thorpe PE,Ross WC,Brown AN,Myers CD,Cumber AJ,Foxwell BM,Forrester JT
Journal:European journal of biochemistry
PubMed ID:6608447
A method is described for preparing specific cytotoxic agents by linking intact ricin to antibodies in a manner that produces obstruction of the galactose-binding sites on the B chain of the toxin and so diminishes the capacity of the conjugate to bind non-specifically to cells. The conjugates were synthesised by ... More
Enhancement of the thrombolytic potency of plasminogen activators by conjugation with clot-specific monoclonal antibodies.
Authors:Dewerchin M, Collen D
Journal:Bioconjug Chem
PubMed ID:1790167
Detection of one attomole of [Arg8]-vasopressin by novel noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay).
Authors:Hashida S, Tanaka K, Yamamoto N, Uno T, Yamaguchi K, Ishikawa E
Journal:J Biochem (Tokyo)
PubMed ID:1778973
'One attomole of [Arg8]-vasopressin (AVP) was detected by a novel noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay). AVP was indirectly biotinylated using N-hydroxysuccinimidobiotin and trapped onto an anti-AVP IgG-coated polystyrene ball. After washing, biotinylated AVP was eluted from the polystyrene ball with HCl and was reacted with 2,4-dinitrophenyl-fluorescein disulfide-bovine ... More
Activation of methotrexate-alpha-alanine by carboxypeptidase A-monoclonal antibody conjugate.
Authors:Haenseler E, Esswein A, Vitols KS, Montejano Y, Mueller BM, Reisfeld RA, Huennekens FM
Journal:Biochemistry
PubMed ID:1731945
'Carboxypeptidase A (CP-A) and monoclonal antibody KS1/4 directed against an antigen on human lung adenocarcinoma cells (UCLA-P3) were derivatized by treatment with succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate and N-succinimidyl 3-(2-pyridyldithio)propionate, respectively. The derivatized proteins were reacted to produce thioether-linked enzyme-antibody conjugates. Sequential HPLC size-exclusion and DEAE chromatography separated the conjugate preparation from unreacted ... More
Selective destruction of nerve growth factor receptor-bearing cells in vitro using a hybrid toxin composed of ricin A chain and a monoclonal antibody against the nerve growth factor receptor.
Authors:DiStefano PS, Schweitzer JB, Taniuchi M, Johnson EM
Journal:J Cell Biol
PubMed ID:2993314
'A hybrid toxin composed of ricin A chain and a monoclonal antibody directed against the rat nerve growth factor (NGF) receptor (192-IgG) was prepared using the heterobifunctional cross-linking agent N-succinimidyl-3-(2-pyridyldithio)-propionate and purified by affinity chromatography. Characterization studies showed that the hybrid, 192-s-s-A, displaced bound 125I-labeled 192-IgG from rat superior cervical ... More
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