Informational:The SiteClick™ Antibody Azido Modification Kit is optimized to be used in conjunction with our SiteClick™ sDIBO Alkyne Kits
Invitrogen™

SiteClick™ Antibody Azido Modification Kit

Catalog number: S20026
Invitrogen™

SiteClick™ Antibody Azido Modification Kit

Catalog number: S20026
Catalog Number
S20026
Unit Size
for 100-250 µg of Ab
Price (USD)
Price: 483.00
Online price: 463.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
for 1 mg of Ab
Price (USD)
Price: 1,112.00
Online price: 1,066.65
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
for 5 mg of Ab
Price (USD)
Price: 3,330.00
Online price: 3,196.65
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
S20026for 100-250 µg of Ab
Price: 483.00
Online price: 463.65
Your price:
-
S10900for 1 mg of Ab
Price: 1,112.00
Online price: 1,066.65
Your price:
-
S10901for 5 mg of Ab
Price: 3,330.00
Online price: 3,196.65
Your price:
-
Product Overview
Figures
Videos
Recommendations
Recommendations
Documents
FAQ
Citations & References
Additional Information
Recommendations

Create a label-ready, site-specific, azido-modified antibody without lengthy and inefficient genetic modification using the SiteClick Antibody Azido Modification Kit. SiteClick labeling uses enzymes to specifically attach an azido moiety to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain unaltered for binding to the antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on the Fc region of essentially all IgG antibodies regardless of isotype and host species.

Once azido–modified, a variety of SiteClick sDIBO or DBCO alkyne labels are available for covalent attachment to the antibody via copper-free click chemistry under mild reaction conditions at your choice of scale. This provides the flexibility to choose different fluorescent labels for your antibody, attach another molecule via streptavidin, or attach your own molecule, such as an oligo, drug, or toxin via amine-reactive or amine-containing moieties depending on your assay. This site-specific modification using SiteClick labeling is a critical tool in the development of therapeutic antibodies, antibody-drug conjugates, biologics, and immunodiagnostic tests that require high specificity with reproducible labeling reactions and helps ensure that antibody performance and function are preserved after conjugation.

Features of the SiteClick Antibody Azido Modification Kit include:
• Contains everything required to azido-modify any species or isotype of IgG antibody
• Easy-to-follow step-by-step protocol
• Highly efficient, site-specific labeling preserves antibody function and results in high quality antibody conjugate
• Reproducible labeling chemistry with lot-to-lot consistency in degree of labeling
• Optimal method for site-specific labeling of therapeutic antibodies, antibody-drug conjugates, biologics, and immunodiagnostics

Custom SiteClick Antibody Labeling Service and sDIBO labels
If you have an antibody that is considered 'difficult to label' or has lost activity after labeling using a conventional method, please contact our custom service representatives to determine whether the SiteClick Antibody Labeling Service would be right for your antibody. We offer complete custom SiteClick antibody labeling services with the option of multiple detection molecules including biotin, Alexa Fluor dyes, Qdot fluorophores, R-PE, chelates for PET imaging, and many others.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
Antibody Azido Modification Kit
Labeling Scale
100 to 250 μg
Shipping Condition
Room Temperature
Product Line
SiteClick™
Chemical Reactivity
Azide
Label or Dye
Azide, Alexa Fluor 555, Alexa Fluor 488
Color
Clear
Labeling Target
Antibodies

Contents & Storage

Store in refrigerator (2–8°C), desiccate, protect from light, and do not freeze.

Figures

Documents & Downloads

Certificates

    Frequently asked questions (FAQs)

    Citations & References

    Search citations by name, author, journal title or abstract text