Create a label-ready, site-specific, azido-modified antibody without lengthy and inefficient genetic modification using the SiteClick Antibody Azido Modification Kit. SiteClick labeling uses enzymes to specifically attach an azido moiety to the heavy chains of an IgG antibody, ensuring that the antigen-binding domains remain unaltered for binding to the antigen target. This site selectivity is achieved by targeting the carbohydrate domains present on the Fc region of essentially all IgG antibodies regardless of isotype and host species.
Once azido–modified, a variety of SiteClick sDIBO or DBCO alkyne labels are available for covalent attachment to the antibody via copper-free click chemistry under mild reaction conditions at your choice of scale. This provides the flexibility to choose different fluorescent labels for your antibody, attach another molecule via streptavidin, or attach your own molecule, such as an oligo, drug, or toxin via amine-reactive or amine-containing moieties depending on your assay. This site-specific modification using SiteClick labeling is a critical tool in the development of therapeutic antibodies, antibody-drug conjugates, biologics, and immunodiagnostic tests that require high specificity with reproducible labeling reactions and helps ensure that antibody performance and function are preserved after conjugation.
Features of the SiteClick Antibody Azido Modification Kit include:
• Contains everything required to azido-modify any species or isotype of IgG antibody
• Easy-to-follow step-by-step protocol
• Highly efficient, site-specific labeling preserves antibody function and results in high quality antibody conjugate
• Reproducible labeling chemistry with lot-to-lot consistency in degree of labeling
• Optimal method for site-specific labeling of therapeutic antibodies, antibody-drug conjugates, biologics, and immunodiagnostics
Custom SiteClick Antibody Labeling Service and sDIBO labels
If you have an antibody that is considered 'difficult to label' or has lost activity after labeling using a conventional method, please contact our custom service representatives to determine whether the SiteClick Antibody Labeling Service would be right for your antibody. We offer complete custom SiteClick antibody labeling services with the option of multiple detection molecules including biotin, Alexa Fluor dyes, Qdot fluorophores, R-PE, chelates for PET imaging, and many others.