Seeing the trees in the forest: selective electroporation of adipocytes within adipose tissue.
AuthorsGranneman JG, Li P, Lu Y, Tilak J
JournalAm J Physiol Endocrinol Metab
PubMed ID15126244
'Electroporation has been recently adapted for the transfer of macromolecules into cells of tissues in vivo. Although mature adipocytes constitute <20% of cells residing in adipose tissue, we hypothesized that fat cells might be susceptible to selective electrotransfer of plasmid DNA owing to their large size relative to other cells ... More
PKC putative phosphorylation site Ser235 is required for MIP/AQP0 translocation to the plasma membrane.
AuthorsGolestaneh N, Fan J, Zelenka P, Chepelinsky AB,
JournalMol Vis
PubMed ID18523655
'PURPOSE: To investigate the functional significance of MIP/AQP0 phosphorylation at serine(235). METHODS: MIP/AQP0 expression and cellular localization was studied in rat lens epithelia explants induced to differentiate by FGF-2. MIP wild type (WT) and MIP (S235A) mutant expression plasmids were constructed and transiently expressed in RK13 cells. Subcellular localization of ... More
Collagen XVI harbors an integrin alpha1 beta1 recognition site in its C-terminal domains.
AuthorsEble JA, Kassner A, Niland S, Mörgelin M, Grifka J, Grässel S
JournalJ Biol Chem
PubMed ID16754661
'Collagen XVI is integrated tissue-dependently into distinct fibrillar aggregates, such as D-banded cartilage fibrils and fibrillin-1-containing microfibrils. In skin, the distribution of collagen XVI overlaps that of the collagen-binding integrins alpha1 beta1 and alpha2 beta1. Basal layer keratinocytes express integrin alpha2 beta1, whereas integrin alpha1 beta1 occurs in smooth muscle ... More
Diabetes is predicted by the beta cell level of autoantigen.
AuthorsByersdorfer CA, Schweitzer GG, Unanue ER
JournalJ Immunol
PubMed ID16177075
'Two novel transgenic (Tg) strains were created expressing hen egg-white lysozyme (HEL) in a pancreas-specific fashion. RmHP.111 mice had levels of HEL per cell similar to that of the established ILK-3 strain, while RmHP.117 mice had 10-fold lower levels (50,000 molecules per cell). When bred to 3A9 TCR Tg mice, ... More
Metabolic and cellular plasticity in white adipose tissue II: role of peroxisome proliferator-activated receptor-alpha.
AuthorsLi P, Zhu Z, Lu Y, Granneman JG
JournalAm J Physiol Endocrinol Metab
PubMed ID15941786
'Chronic activation of adipocyte beta-adrenergic receptors induces remodeling of white adipose tissue (WAT) that includes a transient inflammatory response followed by mitochondrial biogenesis, induction of fatty acid oxidation genes, and elevation of tissue oxidative metabolism. Gene profiling experiments of WAT during remodeling induced by the beta(3)-adrenergic receptor agonist CL-316,243 (CL) ... More
Antibody to Langerin/CD207 localizes large numbers of CD8alpha+ dendritic cells to the marginal zone of mouse spleen.
AuthorsIdoyaga J, Suda N, Suda K, Park CG, Steinman RM,
JournalProc Natl Acad Sci U S A
PubMed ID19168629
Dendritic cells (DCs) are strategically positioned to take up antigens and initiate adaptive immunity. One DC subset expresses CD8alphaalpha in mice and is specialized to capture dying cells and process antigens for MHC class I ... More
Whole-genome genotyping with the single-base extension assay.
We describe an efficient, accurate and robust whole-genome genotyping (WGG) assay based on a two-color, single-base extension (SBE), single-nucleotide polymorphism (SNP)-scoring step. We report genotyping results for biallelic International HapMap quality control (QC) SNPs using a single probe per locus. We show scalability, throughput and accuracy of the system by ... More
Quantum dot ex vivo labeling of neuromuscular synapses.
Nicotinic receptors (nAchRs) are responsible for fast excitatory signaling by the neurotransmitter acetylcholine (Ach). They are present on the postsynaptic membrane at neuromuscular junctions (NMJs) and also at brain synapses. Alpha-bungarotoxin (alpha-BTX), a high-affinity nAchR antagonist, inhibits Ach binding and neurotransmission. Here we demonstrate biotinylated alpha-BTX, bound to native mouse ... More
AuthorsKartalov EP, Zhong JF, Scherer A, Quake SR, Taylor CR, Anderson WF,
JournalBiotechniques
PubMed ID16454045
Here we describe the development of a high-throughput multi-antigen microfluidic fluorescence immunoassay system. A 100-chamber polydimethylsiloxane (PDMS) chip performs up to 5 tests for each of 10 samples. In this particular study system, the specificity of detection was demonstrated, and calibration curves were produced for C-reactive protein (CRP), prostate-specific antigen ... More
Endophilin B1 as a novel regulator of nerve growth factor/ TrkA trafficking and neurite outgrowth.
AuthorsWan J, Cheung AY, Fu WY, Wu C, Zhang M, Mobley WC, Cheung ZH, Ip NY,
JournalJ Neurosci
PubMed ID18768694
Neurotrophins and their cognate receptors Trks are important regulators of neuronal survival and differentiation. Recent studies reveal that internalization and trafficking of Trks play a critical role in neurotrophin-mediated signaling. At present, little is known of the molecular events that mediate this process. In the current study, we show that ... More