Phagocytosis and peroxidase release by seabream (Sparus aurata L.) leucocytes in response to yeast cells.
AuthorsRodrÃguez A, Esteban MA, Meseguer J
JournalAnat Rec A Discov Mol Cell Evol Biol
PubMed ID12704699
A flow cytometric method was adapted to evaluate phagocytosis by gilthead seabream leucocytes after incubation with yeast cells (Saccharomyces cerevisiae). Head-kidney leucocytes were incubated in vitro for different times in different proportions with heat-killed fluorescein isothiocyanate (FITC)-labeled yeast cells to study the kinetics of phagocytosis. Attached and internalized yeast cells ... More
Conserved role of a complement-like protein in phagocytosis revealed by dsRNA knockout in cultured cells of the mosquito, Anopheles gambiae.
AuthorsLevashina EA, Moita LF, Blandin S, Vriend G, Lagueux M, Kafatos FC
JournalCell
PubMed ID11257225
'We characterize a novel hemocyte-specific acute phase glycoprotein from the malaria vector, Anopheles gambiae. It shows substantial structural and functional similarities, including the highly conserved thioester motif, to both a central component of mammalian complement system, factor C3, and to a pan-protease inhibitor, alpha2-macroglobulin. Most importantly, this protein serves as ... More
Simultaneous flow cytometric method to measure phagocytosis and oxidative products by neutrophils.
AuthorsPerticarari S, Presani G, Mangiarotti MA, Banfi E
JournalCytometry
PubMed ID1782835
'We developed a rapid and sensitive two-color flow cytometric method which allows the simultaneous quantification of both the phagocytosis rate and the oxidative burst activation of polymorphonuclear leukocytes (PMNLs). The oxidation of hydroethidine (HE) to ethidium bromide (EB) was performed by the oxidative neutrophil products within the cells during the ... More
Increase in phagocytosis after geldanamycin treatment or heat shock: role of heat shock proteins.
AuthorsVega VL, De Maio A
JournalJ Immunol
PubMed ID16210633
'The response to injury is activated at the systemic and cellular levels. At the systemic level, phagocytosis plays a key role in controlling infections and clearing necrotic and apoptotic cells. The expression of heat shock proteins (Hsp), which is a well-conserved process, is a major component of cellular response to ... More
Quantitation of phagocytosis by confocal microscopy.
AuthorsBabcock GF
JournalMethods Enzymol
PubMed ID10506981
'Confocal microscopy is an excellent tool to quantify phagocytosis. Depending on the particle used, phagocytosis can be determined by the simple manual counting of internalized particles. If a fluorescence probe is utilized, an analysis of fluorescence intensity can be used for quantification. The basic procedure can be altered in a ... More
Phagocytosis by human leukocytes, phagosomal pH and degradation of seven species of bacteria measured by flow cytometry.
AuthorsBassøe CF, Bjerknes R
JournalJ Med Microbiol
PubMed ID3968704
'Phagocytosis by human leukocytes, phagosomal pH and degradation of seven species of bacteria were studied by a flow cytometric method. The percentage of phagocytosing leukocytes was similar for all bacterial strains examined, but Salmonella typhi and Neisseria meningitidis were more slowly phagocytosed than other bacteria. The phagosomal pH surrounding the ... More
Rapid whole-blood microassay using flow cytometry for measuring neutrophil phagocytosis.
AuthorsWhite-Owen C, Alexander JW, Sramkoski RM, Babcock GF
JournalJ Clin Microbiol
PubMed ID1500514
'A simple flow cytometric method (FCM) for measuring phagocytosis of Staphylococcus aureus by human neutrophils (polymorphonuclear leukocytes [PMNs]) is described. This assay utilizes 100 microliters of EDTA-anticoagulated whole blood and a simplified method of fluorescently labeling bacteria. A commercially available whole-blood lysing reagent allows for the removal of erythrocytes and ... More
A new flow cytometric assay for the evaluation of phagocytosis and the oxidative burst in whole blood.
AuthorsPerticarari S, Presani G, Banfi E
JournalJ Immunol Methods
PubMed ID8157984
'Phagocytes play an important role in host defence against microorganisms and different techniques are needed to evaluate their functional activities. Here we describe a rapid, simple and reliable one step procedure to measure the phagocytosis rate and oxidative burst activation of both polymorphonuclear leukocytes (PMN) and monocytes, by means of ... More
Fluorescent staphylococci as microbeads.
AuthorsSzabó G, Damjanovich S
JournalCytometry
PubMed ID2684580
'Fixed bacteria of the protein A-rich Cowan I Staphylococcus strain were labelled with fluorescein isothiocyanate and used as the second-step reagent in an indirect immunofluorescent assay of specific cell-surface antigen expression. The results are documented with fluorescence microscopy and flow cytometry.' ... More
Staphylococcus aureus phagocytosis. A new cytofluorometric method using FITC and paraformaldehyde.
AuthorsCantinieaux B, Hariga C, Courtoy P, Hupin J, Fondu P
JournalJ Immunol Methods
PubMed ID2503560
'A new method for measuring the uptake of Staphylococcus aureus by human polymorphonuclear neutrophils (PMN) using flow cytometry (FCM) is described. Bacteria were labeled with fluorescein isothiocyanate (FITC) and incubated with PMN in a suspension assay. At the end of the assay, phagocytosis was arrested by the addition of cold ... More
Phagocytosis of bacteria by human leukocytes measured by flow cytometry.
AuthorsBassøe CF, Laerum OD, Solberg CO, Haneberg B
JournalProc Soc Exp Biol Med
PubMed ID6415659
A new method has been developed for the evaluation of the phagocytic activity of human leukocytes using fluorescently labeled bacteria and flow cytometry. By simultaneous measurement of cellular light scatter and fluorescence, extracellular bacteria, phagocytes, and nonphagocytes could be discriminated and quantified. All leukocytes assumed to be capable of phagocytosis ... More
Surfactant protein A (SP-A)-mediated clearance of Staphylococcus aureus involves binding of SP-A to the staphylococcal adhesin eap and the macrophage receptors SP-A receptor 210 and scavenger receptor class A.
AuthorsSever-Chroneos Z, Krupa A, Davis J, Hasan M, Yang CH, Szeliga J, Herrmann M, Hussain M, Geisbrecht BV, Kobzik L, Chroneos ZC,
JournalJ Biol Chem
PubMed ID21123169
Staphylococcus aureus causes life-threatening pneumonia in hospitals and deadly superinfection during viral influenza. The current study investigated the role of surfactant protein A (SP-A) in opsonization and clearance of S. aureus. Previous studies showed that SP-A mediates phagocytosis via the SP-A receptor 210 (SP-R210). Here, we show that SP-R210 mediates ... More
gamma-tubulin is a minus end-specific microtubule binding protein.
AuthorsLi Q, Joshi HC
JournalJ Cell Biol
PubMed ID7559777
The role of microtubules in mediating chromosome segregation during mitosis is well-recognized. In addition, interphase cells depend upon a radial and uniform orientation of microtubules, which are intrinsically asymmetric polymers, for the directional transport of many cytoplasmic components and for the maintenance of the structural integrity of certain organelles. The ... More
Supplemental L-arginine HCl augments bacterial phagocytosis in human polymorphonuclear leukocytes.
AuthorsMoffat FL, Han T, Li ZM, Peck MD, Jy W, Ahn YS, Chu AJ, Bourguignon LY
JournalJ Cell Physiol
PubMed ID8647919
That L-arginine (L-Arg) augments the host response to acute bacterial sepsis suggests that this amino acid intervenes early in the immune response, perhaps via the nitric oxide synthetase (NOS) pathway. The effect of L-Arg supplementation on in vitro phagocytosis of fluorescein-labeled, heat-killed Staphylococcus aureus by peripheral blood neutrophils (PMNs) from ... More
Phagosomal acidification is mediated by a vacuolar-type H(+)-ATPase in murine macrophages.
AuthorsLukacs GL, Rotstein OD, Grinstein S
JournalJ Biol Chem
PubMed ID2147429
The mechanism underlying phagosomal acidification was studied in thioglycolate-elicited murine macrophages. The pH of the phagosomal compartment (pHp) was measured fluorimetrically in macrophage suspensions following ingestion of fluorescein isothiocyanate-labeled Staphylococcus aureus. At 37 degrees C, pHp decreased rapidly, reaching a steady state value of 5.8-6.1, while the cytoplasmic pH remained ... More