Streptavidin, Alexa Fluor™ 405 conjugate
Invitrogen™

Streptavidin, Alexa Fluor™ 405 conjugate

Unser Streptavidin, Alexa Fluor 405 Konjugat ist eine helle, photostabile Sonde mit Anregungs-/Emissionsmaxima von 402 bzw. 421 nm. Streptavidin istWeitere Informationen
Have Questions?
KatalognummerMenge
S323511 mg
Katalognummer S32351
Preis (EUR)
472,00
Each
Menge:
1 mg
Preis (EUR)
472,00
Each
Unser Streptavidin, Alexa Fluor 405 Konjugat ist eine helle, photostabile Sonde mit Anregungs-/Emissionsmaxima von 402 bzw. 421 nm. Streptavidin ist ein nicht glykosyliertes Biotin-bindendes Protein, das aus Bakterien isoliert ist und einen nahezu neutralen isoelektrischen Punkt hat. Es wird häufig verwendet, um biotinylierte Sonden nachzuweisen, da es Berichten zufolge weniger unspezifische Bindungen aufweist als das glykosylierte, Biotin-bindende Protein Avidin.

Sehen Sie sich eine Auswahlhilfe für alle Alexa Fluor™ Streptavidin- und Avidin-Konjugate an.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
NachweisverfahrenFluoreszent
Anregung/Emission402/421
Durchflusszytometer-Laserlinien405
Zur Verwendung mit (Geräte)Durchflusszytometrie, Immunassays, histochemische Anwendungen, Blot-Analyse
Marker oder FarbstoffAlexa Fluor 405
ProdukttypStreptavidin-Konjugat (fluoreszierend)
Menge1 mg
VersandbedingungRaumtemperatur
KonjugatAlexa Fluor 405
FormFest
ProduktlinieAlexa Fluor
Unit SizeEach
Inhalt und Lagerung
Enthält 1 Fläschchen Streptavidin Alexa Fluor™ 405 Konjugat.

Im Tiefkühlgerät bei -5 bis -30 °C und vor Licht geschützt lagern.

Häufig gestellte Fragen (FAQ)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

Zitierungen und Referenzen (4)

Zitierungen und Referenzen
Abstract
Violet laser diodes in flow cytometry: an update.
Authors:Telford W, Kapoor V, Jackson J, Burgess W, Buller G, Hawley T, Hawley R,
Journal:Cytometry A
PubMed ID:17051581
INTRODUCTION: In previous studies we and others have demonstrated the usefulness of violet laser diodes (VLDs) as replacement laser sources for krypton-ion lasers on stream-in-air cytometers. Previously available VLDs had a maximum available power of less than 25 mW; this was sufficient for excitation of densely labeled cell surface antigens ... More
Novel strategy to selectively label excitatory and inhibitory neurons in the cerebral cortex of mice.
Authors:Basu K, Gravel C, Tomioka R, Kaneko T, Tamamaki N, Sík A,
Journal:J Neurosci Methods
PubMed ID:18321591
Revealing the connections of neuronal systems is critical for understanding how they function. The vast majority of neurons in all cortical areas consist of excitatory cells whose activity is controlled by inhibitory cells. Distribution and projection patterns of inhibitory and excitatory cells are key information to understand the organization of ... More
Endosomal trafficking of the ligated FcvarepsilonRI receptor.
Authors:Fattakhova GV, Masilamani M, Narayanan S, Borrego F, Gilfillan AM, Metcalfe DD, Coligan JE,
Journal:Mol Immunol
PubMed ID:18945491
In addition to initiating signaling cascades leading to mast cell mediator release, aggregation of the high affinity IgE receptor (FcvarepsilonRI) leads to rapid internalization of the cross-linked receptor. However, little is known about the trafficking of the internalized FcvarepsilonRI. Here we demonstrate that in RBL-2H3 cells, aggregated FcvarepsilonRI appears in ... More
Inducible gene targeting in the neonatal vasculature and analysis of retinal angiogenesis in mice.
Authors:Pitulescu ME, Schmidt I, Benedito R, Adams RH,
Journal:Nat Protoc
PubMed ID:20725067
The retina is a powerful experimental system for the analysis of angiogenic blood vessel growth in the postnatal organisms. The three-dimensional architecture of the vessel network and processes as diverse as endothelial cell (EC) proliferation, sprouting, perivascular cell recruitment, vessel remodeling or maturation can be investigated at high resolution. The ... More