Streptavidin, Allophycocyanin Conjugate, premium grade
Streptavidin, Allophycocyanin Conjugate, premium grade
Citas y referencias (88)
Invitrogen™

Streptavidin, Allophycocyanin Conjugate, premium grade

La estreptavidina es una proteína de unión a biotina derivada bacteriológicamente que se ha constatado que exhibe menos unión inespecíficaMás información
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Número de catálogoCantidad
S32362
también denominado S-32362
250 μL
Número de catálogo S32362
también denominado S-32362
Precio (MXN)
-
Cantidad:
250 μL
La estreptavidina es una proteína de unión a biotina derivada bacteriológicamente que se ha constatado que exhibe menos unión inespecífica que la avidina. La aloficocianina es una ficobiliproteína de brillo intenso con un amplio perfil de absorción que alcanza un pico de ∼ 650 nm y una emisión de fluorescencia máxima de ∼ 660 nm. Nuestro conjugado de estreptavidina y aloficocianina de *alta calidad* es un fraccionamiento adicional de nuestro producto S-868, destinado especialmente para aplicaciones que se benefician de una pureza adicional.

Consulte la guía de selección para todos los conjugados de estreptavidina RPE, APC y tándem.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración1 mg/mL
Método de detecciónFluorescente
Excitación/emisión650/660
Líneas láser del citómetro de flujo633 o 635
Para utilizar con (equipo)Citometría de flujo, inmunoensayos, aplicaciones histoquímicas, análisis de blot
Etiqueta o tinteAPC (Aloficocianina)
Tipo de productoConjugado de estreptavidina (Fluorescente)
Cantidad250 μL
Condiciones de envíoHielo húmedo
ConjugadoAPC
FormularioSolución
Unit SizeEach
Contenido y almacenamiento
Contiene 1 vial de conjugado de estreptavidina APC de alta calidad (1 mg/ml).

Almacenar en el refrigerador (2–8 °C) y proteger de la luz.

Preguntas frecuentes

For Streptavidin, Allophycocyanin Conjugate, premium grade (Cat. No. S32362), how many molecules of APC are conjugated to each streptavidin molecule?

Streptavidin APC, premium grade has a degree of labeling of one APC molecule to one streptavidin molecule.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How should I store my allophycocyanin (APC)-conjugated antibody?

Allophycocyanin-conjugated antibodies must be stored at 2-8 degrees C. Antibodies conjugated to phycobiliproteins (APC, R-PE) must never be frozen. Once frozen, even briefly, they are no longer usable.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (88)

Citations & References
Abstract
Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers.
Authors:Chattopadhyay PK, Melenhorst JJ, Ladell K, Gostick E, Scheinberg P, Barrett AJ, Wooldridge L, Roederer M, Sewell AK, Price DA,
Journal:Cytometry A
PubMed ID:18836993
'The ability to quantify and characterize antigen-specific CD8+ T cells irrespective of functional readouts using fluorochrome-conjugated peptide-major histocompatibility complex class I (pMHCI) tetramers in conjunction with flow cytometry has transformed our understanding of cellular immune responses over the past decade. In the case of prevalent CD8+ T cell populations that ... More
Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation.
Authors:Hirsch JD, Eslamizar L, Filanoski BJ, Malekzadeh N, Haugland RP, Beechem JM, Haugland RP
Journal:Anal Biochem
PubMed ID:12419349
'The high-affinity binding of biotin to avidin, streptavidin, and related proteins has been exploited for decades. However, a disadvantage of the biotin/biotin-binding protein interaction is that it is essentially irreversible under physiological conditions. Desthiobiotin is a biotin analogue that binds less tightly to biotin-binding proteins and is easily displaced by ... More
Nonradioactive methods for the assay of phosphoinositide 3-kinases and phosphoinositide phosphatases and selective detection of signaling lipids in cell and tissue extracts.
Authors:Gray A, Olsson H, Batty IH, Priganica L, Peter Downes C
Journal:Anal Biochem
PubMed ID:12605860
'We describe a novel approach to quantitation of phosphoinositides in cell extracts and in vitro enzyme-catalyzed reactions using suitably tagged and/or labeled pleckstrin homology (PH) domains as probes. Stable complexes were formed between the biotinylated target lipid and an appropriate PH domain, and phosphoinositides present in samples were detected by ... More
Development of a ubiquitin transfer assay for high throughput screening by fluorescence resonance energy transfer.
Authors:Boisclair MD, McClure C, Josiah S, Glass S, Bottomley S, Kamerkar S, Hemmilä I
Journal:J Biomol Screen
PubMed ID:11080690
'An assay based on fluorescence resonance energy transfer (FRET) has been developed to screen for ubiquitination inhibitors. The assay measures the transfer of ubiquitin from Ubc4 to HECT protein Rsc 1083. Secondary reagents (streptavidin and antibody to glutathione-S-transferase [GST]), pre-labeled with fluorophores (europium chelate, Eu(3+), and allophycocyanin [APC]), are noncovalently ... More
Cell surface markers in human embryonic stem cells.
Authors:Rao RR, Johnson AV, Stice SL,
Journal:Methods Mol Biol
PubMed ID:18453248
'The pluripotent nature of human embryonic stem cells (hESCs) is based on their potential to form every cell type in the body. Prior to use in directed differentiation strategies, these cells need to be thoroughly characterized. The large number of glycoproteins and carbohydrates that exist on the cell surface provide ... More
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