Streptavidin, Pacific Orange™ conjugate
Streptavidin, Pacific Orange™ conjugate
Citas y referencias (2)
Invitrogen™

Streptavidin, Pacific Orange™ conjugate

La estreptavidina Pacific Orange™ tiene absorción/emisión máxima de ∼ 400/551 nm. La estreptavidina es una proteína no glucosilada de uniónMás información
Have Questions?
Número de catálogoCantidad
S323651 mg
Número de catálogo S32365
Precio (MXN)
-
Cantidad:
1 mg
La estreptavidina Pacific Orange™ tiene absorción/emisión máxima de ∼ 400/551 nm. La estreptavidina es una proteína no glucosilada de unión de biotina que se aísla de bacterias y tiene un punto isoeléctrico casi neutro. Se utiliza ampliamente para detectar sondas biotiniladas, porque se ha constatado que exhibe una unión menos inespecífica que la avidina, una proteína glucosilada de unión de biotina.

Consulte la guía de selección para ver todos los conjugados de estreptavidina y avidina Alexa Fluor™.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Excitación/emisión400/551
Líneas láser del citómetro de flujo405
Para utilizar con (equipo)Citometría de flujo, inmunoensayos, aplicaciones histoquímicas, análisis de blot
Etiqueta o tintePacific Orange
Tipo de productoConjugado de estreptavidina (Fluorescente)
Cantidad1 mg
Condiciones de envíoTemperatura ambiente
ConjugadoPacific Orange
FormularioSólido
Línea de productosPacific Orange
Unit SizeEach
Contenido y almacenamiento
Contiene 1 vial de conjugado de estreptavidina Pacific Orange™.

Almacenar en el congelador (de -5 a -30 °C) y proteger de la luz.

Preguntas frecuentes

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

Citations & References (2)

Citations & References
Abstract
An 11-color flow cytometric assay for identifying, phenotyping, and assessing endocytic ability of peripheral blood dendritic cell subsets in a single platform.
Authors:Wang JC, Kobie JJ, Zhang L, Cochran M, Mosmann TR, Ritchlin CT, Quataert SA,
Journal:J Immunol Methods
PubMed ID:19049809
Human peripheral blood dendritic cells (PBDC) are a rare population comprised of several distinctive subsets. Analysis of these cells has been hindered by their low frequency. In this study, we report a novel direct ex vivo 11-color flow cytometric assay that combines subset identification with analysis of activation status and ... More
Violet laser diodes in flow cytometry: an update.
Authors:Telford W, Kapoor V, Jackson J, Burgess W, Buller G, Hawley T, Hawley R,
Journal:Cytometry A
PubMed ID:17051581
INTRODUCTION: In previous studies we and others have demonstrated the usefulness of violet laser diodes (VLDs) as replacement laser sources for krypton-ion lasers on stream-in-air cytometers. Previously available VLDs had a maximum available power of less than 25 mW; this was sufficient for excitation of densely labeled cell surface antigens ... More