Citations & References (2)

Invitrogen™

Streptavidin, Pacific Orange™ conjugate

Catalog Number	Quantity
S32365	1 mg

Catalog number S32365

Price (USD)

339.00

Quantity:

Price (USD)

339.00

Pacific Orange™ streptavidin has absorption/emission maxima of ∼400/551 nm. Streptavidin is a nonglycosylated biotin-binding protein that is isolated from bacteria and has a near-neutral isoelectric point. It is widely used to detect biotinylated probes, because it reportedly exhibits less nonspecific binding than does the glycosylated, biotin-binding protein, avidin.

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View a selection guide for all Alexa Fluor™ streptavidin and avidin conjugates.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection MethodFluorescence

Excitation/Emission400/551

Flow Cytometer Laser Lines405

For Use With (Equipment)Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis

Label or DyePacific Orange

Product TypeStreptavidin Conjugate (Fluorescent)

Quantity1 mg

Shipping ConditionRoom Temperature

ConjugatePacific Orange

FormSolid

Product LinePacific Orange

Unit SizeEach

Contents & Storage

Contains 1 vial of streptavidin Pacific Orange™ conjugate.

Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

Citations & References (2)

Citations & References

Abstract

An 11-color flow cytometric assay for identifying, phenotyping, and assessing endocytic ability of peripheral blood dendritic cell subsets in a single platform.

Authors:Wang JC, Kobie JJ, Zhang L, Cochran M, Mosmann TR, Ritchlin CT, Quataert SA,

Journal:J Immunol Methods

PubMed ID:19049809

Human peripheral blood dendritic cells (PBDC) are a rare population comprised of several distinctive subsets. Analysis of these cells has been hindered by their low frequency. In this study, we report a novel direct ex vivo 11-color flow cytometric assay that combines subset identification with analysis of activation status and ... More

Violet laser diodes in flow cytometry: an update.

Authors:Telford W, Kapoor V, Jackson J, Burgess W, Buller G, Hawley T, Hawley R,

Journal:Cytometry A

PubMed ID:17051581

INTRODUCTION: In previous studies we and others have demonstrated the usefulness of violet laser diodes (VLDs) as replacement laser sources for krypton-ion lasers on stream-in-air cytometers. Previously available VLDs had a maximum available power of less than 25 mW; this was sufficient for excitation of densely labeled cell surface antigens ... More