SYTOX™ Blue Dead Cell Stain, for flow cytometry

Description

SYTOX™ Blue dead cell stain is a simple and quantitative single-step dead-cell indicator for use with violet laser equipped flow cytometers. SYTOX™ Blue dead cell stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes but will not cross uncompromised cell membranes. After brief incubation with SYTOX™ Blue stain, the nucleic acids of dead cells fluoresce bright blue when excited with 405 nm violet laser light. The violet-excited fluorescence emission of SYTOX™ Blue stain permits clear discrimination from probes excited by most other laser lines, facilitating the development of multicolor assays with minimal spectral overlap between signals. SYTOX™ Blue dead cell stain is supplied as a 1 mM solution in dimethylsulfoxide (DMSO) in a unit size of 1 mL.

View a selection guide for all nonfixable viability dyes for flow cytometry.

Figures

Specifications

For Use With (Equipment):	Flow Cytometer
Cell Permeability:	Cell-Impermeant
Sub-Cellular Localization:	Nucleic Acids, Nucleus
Detection Method:	Fluorescent
Excitation⁄Emission (nm):	444⁄480
Flow Cytometer Laser Lines:	405
Form:	Solution
Format:	Tube(s)
Label or Dye:	SYTOX® Blue
Number of Reactions:	1,000
Product Line:	SYTOX®
Product Size:	1 mL
Shipping Condition:	Room Temperature

Contents & storage