Invitrogen™

SYTOX™ Blue Dead Cell Stain, for flow cytometry

Back to top

Description

SYTOX™ Blue dead cell stain is a simple and quantitative single-step dead-cell indicator for use with violet laser equipped flow cytometers. SYTOX™ Blue dead cell stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes but will not cross uncompromised cell membranes. After brief incubation with SYTOX™ Blue stain, the nucleic acids of dead cells fluoresce bright blue when excited with 405 nm violet laser light. The violet-excited fluorescence emission of SYTOX™ Blue stain permits clear discrimination from probes excited by most other laser lines, facilitating the development of multicolor assays with minimal spectral overlap between signals. SYTOX™ Blue dead cell stain is supplied as a 1 mM solution in dimethylsulfoxide (DMSO) in a unit size of 1 mL.

View a selection guide for all nonfixable viability dyes for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Permeability: Cell-Impermeant
Detection Method: Fluorescent
Excitation/Emission: 444⁄480
Flow Cytometer Laser Lines: 405
For Use With (Equipment): Flow Cytometer
Form: Solution
Label or Dye: SYTOX® Blue
No. of Reactions: 1,000
Product Line: SYTOX®
Shipping Condition: Room Temperature
Sub Cellular Localization: Nucleus, Nucleic Acids
Format: Tube(s)
Concentration: 1 mM

Contents & storage

Contains 1 vial of SYTOX® blue dead cell stain (1 mM solution in DMSO).

Store in freezer (-5 to -30°C), dessicated and protect from light.