Invitrogen™
SYTOX™ Blue Dead Cell Stain, for flow cytometry
Description
SYTOX™ Blue dead cell stain is a simple and quantitative single-step dead-cell indicator for use with violet laser equipped flow cytometers. SYTOX™ Blue dead cell stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes but will not cross uncompromised cell membranes. After brief incubation with SYTOX™ Blue stain, the nucleic acids of dead cells fluoresce bright blue when excited with 405 nm violet laser light. The violet-excited fluorescence emission of SYTOX™ Blue stain permits clear discrimination from probes excited by most other laser lines, facilitating the development of multicolor assays with minimal spectral overlap between signals. SYTOX™ Blue dead cell stain is supplied as a 1 mM solution in dimethylsulfoxide (DMSO) in a unit size of 1 mL.View a selection guide for all nonfixable viability dyes for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Specifications
| Cell Permeability: | Cell-Impermeant |
|---|---|
| Detection Method: | Fluorescent |
| Excitation/Emission: | 444⁄480 |
| Flow Cytometer Laser Lines: | 405 |
| For Use With (Equipment): | Flow Cytometer |
| Form: | Solution |
| Label or Dye: | SYTOX® Blue |
| No. of Reactions: | 1,000 |
| Product Line: | SYTOX® |
| Shipping Condition: | Room Temperature |
| Sub Cellular Localization: | Nucleus, Nucleic Acids |
| Format: | Tube(s) |
| Concentration: | 1 mM |
Contents & storage
Contains 1 vial of SYTOX® blue dead cell stain (1 mM solution in DMSO).
Store in freezer (-5 to -30°C), dessicated and protect from light.
Store in freezer (-5 to -30°C), dessicated and protect from light.
