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SYTOX® Red Dead Cell Stain, for 633 or 635 nm excitation

Catalog number: S34859

Molecular Probes™  Related applications: Cell Structure | Cell Tracing & Tracking | Cell Viability, Proliferation & Function | Flow Cytometry

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Description

SYTOX™ Red dead cell stain is a simple and quantitative single-step dead-cell indicator for use with red laser-equipped flow cytometers. SYTOX™ Red dead cell stain is a high-affinity nucleic acid stain that easily penetrates cells with compromised plasma membranes but will not cross uncompromised cell membranes. After brief incubation with SYTOX™ Red stain, the nucleic acids of dead cells fluoresce bright red when excited with 633 or 635 nm red laser light. SYTOX™ Red dead cell stain is distinct from other dead cell probes like 7-AAD and PI, which are excited using 488 nm. The emission of SYTOX? Red stain is limited to one channel with minimal spectral overlap. This effectively frees all the channels of the 488 nm laser line. SYTOX™ Red dead cell stain is supplied as a 5 µM solution in dimethylsulfoxide (DMSO) in a unit size of 1 mL.

View a selection guide for all nonfixable viability dyes for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Flow Cytometer
Cell Permeability: Cell-Impermeant
Sub-Cellular Localization: Nucleus, Nucleic Acids
Form: Solution
Format: Tube(s)
Label or Dye: SYTOX® Red
Product Size: 1 mL
Detection Method: Fluorescent
Number of Reactions: 1,000
Flow Cytometer Laser Lines: 633/635
Excitation⁄Emission (nm): 640⁄658
Shipping Condition: Room Temperature

Contents & storage

Contains 1 vial of SYTOX® red dead cell stain (5 µM solution in DMSO).

Store in freezer (-5 to -30°C), and protect from light.

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Manuals & protocols

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