Invitrogen™
SYTOX™ Orange Dead Cell Stain, for flow cytometry
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Description
SYTOX™ Orange Dead Cell Stain is a bright, easy-to-use nucleic acid stain for distinguishing dead from live cells in flow cytometry assays. It is excitable at 532 nm with an emission profile similar to R-PE, and has been tested in an assortment of cell types.View a selection guide for all nonfixable viability dyes for flow cytometry.
You get these advantages with SYTOX™ Orange Dead Cell Stain:
• Bright Signal—Easily distinguishes dead cells from live cells.
• Rapid Staining—Single step, No Wash Assay to assess viability of unfixed cells.
• Validation—in Jurkat, ReH, CHO, Kg1a, 3T3, HeLa, HEK (primary cells), and lysed whole blood.
• Optimization—Dye concentration has been optimized for use in flow cytometry.
• Versatility—Tested in a variety of experimental applications.
A bright stain in an easy-to-use, no-wash assay
SYTOX™ Orange Dead Cell Stain is a cell-impermeant nucleic acid stain. By flow cytometry, dead cells can be easily distinguished from live cells by the increased fluorescent signal due to dsDNA binding of SYTOX™ Orange. Because it is non-fluorescent in aqueous media, SYTOX™ Orange Dead Cell Stain can be applied to cells and visualized with minimal background staining and without an additional wash step.
Validated in flow cytometry
SYTOX™ Orange Dead Cell Stain is designed for flow cytometry using the 488 nm blue laser or 532 nm green laser with emission in the 575/26 BP filter (similar to PE). The dye concentration has been optimized for samples with concentrations ranging from 1x104to 1x106 cells per ml.
SYTOX™ Orange Dead Cell Stain has been validated in several cell types including:
• Jurkat
• ReH
• CHO
• Kg1a
• 3T3
• HeLa
• HEK (primary cells)
• Lysed whole blood
SYTOX™ Orange Dead Cell Stain has been tested in several applications including:
• Apoptosis
• Cell cycle
• Cell proliferation
• Immunophenotyping assays
Part of the SYTOX™ family of cell viability stains
SYTOX™ Dead Cell Stains come in an assortment of excitation/emission characteristics and have been incorporated into a number of assays for apoptosis, cell viability, and metabolism.
• SYTOX™ Blue Dead Cell Stain (S34857): 405 nm violet laser.
• SYTOX™ AADvanced™ Dead Cell Stain (S10274, S10309): 488 nm laser with red emission.
• SYTOX™ Red Dead Cell Stain (S34854): 633/5 nm red laser.
• SYTOX™ Green Dead Cell Stain (S34861): 488 nm laser—green emission.
• SYTOX™ Dead Cell Stain Sampler Kit (S34862)
Related Link
• Learn about all SYTOX™ Products.
For Research Use Only. Not for use in diagnostic procedures.
Figures
Specifications
| For Use With (Equipment): | Flow Cytometer |
|---|---|
| Cell Permeability: | Cell-Impermeant |
| Detection Method: | Fluorescent |
| Excitation⁄Emission (nm): | 547⁄570 |
| Flow Cytometer Laser Lines: | 488, 532 |
| Form: | Solution |
| Format: | Tube(s) |
| Label or Dye: | SYTOX® Orange |
| Number of Reactions: | 1,000 |
| Product Line: | SYTOX® |
| Product Size: | 1 mL |
| Shipping Condition: | Room Temperature |
Contents & storage
Contains 1 vial of SYTOX® orange dead cell stain (250 µM solution in DMSO).
Store in freezer (-5 to -30°C), and protect from light.
Store in freezer (-5 to -30°C), and protect from light.
