SYTOX® Orange Dead Cell Stain, for flow cytometry

Description

SYTOX™ Orange Dead Cell Stain is a bright, easy-to-use nucleic acid stain for distinguishing dead from live cells in flow cytometry assays. It is excitable at 532 nm with an emission profile similar to R-PE, and has been tested in an assortment of cell types.

View a selection guide for all nonfixable viability dyes for flow cytometry.

You get these advantages with SYTOX™ Orange Dead Cell Stain:

• Bright Signal—Easily distinguishes dead cells from live cells.
• Rapid Staining—Single step, No Wash Assay to assess viability of unfixed cells.
• Validation—in Jurkat, ReH, CHO, Kg1a, 3T3, HeLa, HEK (primary cells), and lysed whole blood.
• Optimization—Dye concentration has been optimized for use in flow cytometry.
• Versatility—Tested in a variety of experimental applications.

A bright stain in an easy-to-use, no-wash assay
SYTOX™ Orange Dead Cell Stain is a cell-impermeant nucleic acid stain. By flow cytometry, dead cells can be easily distinguished from live cells by the increased fluorescent signal due to dsDNA binding of SYTOX™ Orange. Because it is non-fluorescent in aqueous media, SYTOX™ Orange Dead Cell Stain can be applied to cells and visualized with minimal background staining and without an additional wash step.

Validated in flow cytometry
SYTOX™ Orange Dead Cell Stain is designed for flow cytometry using the 488 nm blue laser or 532 nm green laser with emission in the 575/26 BP filter (similar to PE). The dye concentration has been optimized for samples with concentrations ranging from 1x10⁴to 1x10⁶ cells per ml.

SYTOX™ Orange Dead Cell Stain has been validated in several cell types including:
•  Jurkat
•  ReH
•  CHO
•  Kg1a
•  3T3
•  HeLa
•  HEK (primary cells)
•  Lysed whole blood

SYTOX™ Orange Dead Cell Stain has been tested in several applications including:
•  Apoptosis
•  Cell cycle
•  Cell proliferation
•  Immunophenotyping assays

Part of the SYTOX™ family of cell viability stains
SYTOX™ Dead Cell Stains come in an assortment of excitation/emission characteristics and have been incorporated into a number of assays for apoptosis, cell viability, and metabolism.
•  SYTOX™ Blue Dead Cell Stain (S34857): 405 nm violet laser.
•  SYTOX™ AADvanced™ Dead Cell Stain (S10274, S10309): 488 nm laser with red emission.
•  SYTOX™ Red Dead Cell Stain (S34854): 633/5 nm red laser.
•  SYTOX™ Green Dead Cell Stain (S34861): 488 nm laser—green emission.
•  SYTOX™ Dead Cell Stain Sampler Kit (S34862)

Related Link
•  Learn about all SYTOX™ Products.