SlowFade® Gold Antifade Mountant with DAPI

Description

SlowFade® Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments. SlowFade® Gold Antifade Mountant is glycerol-based, with no mixing or curing required, which makes it ideal for immediate viewing of the sample. It comes ready-to-use—just apply a drop to the sample, add a coverslip, and image. It is available with or without DAPI nuclear stain.

SlowFade® Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins, such as GFP. For superior antifade protection of fluorescent proteins and fluorescent dyes, SlowFade® Diamond Antifade Mountant is recommended. Learn more about key differences between SlowFade® Gold and SlowFade® Diamond antifade mountants.

Key Attributes:

• Protects dyes from fading during imaging
• Ready-to-use liquid, ideal for immediate sample viewing
• Mounted samples are stable for weeks
• Maintains signal strength—little to no quenching
• Ideal for Alexa Fluor® dyes

Get Photobleaching Protection Without Quenching

The antifade reagents used in some mountants can destroy the initial strength of many fluorophores (especially those that emit above 550 nm) by up to 80%. To create the Molecular Probes® Gold series of mountants, our scientists developed a formula of chemical additives that minimize this initial signal quenching for the broadest range of colors and dyes.

Immediate Viewing with Maximum Signal Protection
While epoxy-based mountants used in our FluoCells® series of prepared cells can be stable for years and afford the highest level of antifade protection, they are difficult to use, requiring a series of steps in organic solvents prior to mounting. SlowFade® Gold Antifade Mountant is the best option for immediate viewing of samples. For longer-term storage, it is recommended that a sealant (i.e., finger nail polish or equivalent acrylic reagent) be applied to the coverslip edges to prevent drying. Alternatively, choose our curing mountant ProLong® Gold Antifade Mountant.

Improved Resolution in Thick Sections
Glycerol-based mountants are recommended when viewing thick samples, as the refractive index of glycerol is closer to that of tissue. It's easy to use—just air-dry your stained samples to remove excess water and mount.

Specifications

Reagent Type:	Anti-Fade Solution
Product Size:	2 mL
Green Features:	Less hazardous, Sustainable packaging

Contents & storage