SYBR® Green I Nucleic Acid Gel Stain is one of the most sensitive stains available for detecting double-stranded DNA (dsDNA) in agarose and polyacrylamide gels. Because SYBR® Green I has greater sensitivity for dsDNA, it is especially useful for assays where the presence of contaminating RNA or ssDNA might obscure results. With exceptionally low background fluorescence and spectral characteristics that closely match light sources and filter sets in existing instruments, SYBR® Green I stain is ideal for use with laser scanners. SYBR® Green I stain provides:
• Sensitivity—at least four times greater than ethidium bromide for DNA in agarose gels • Ease of use—gels soaked in diluted stain can be visualized without desalting • Compatibility—with UV transilluminators, gel documentation systems, and laser scanners • Flexibility—for use with a broad range of applications, including DNA typing, PCR-based assays, DNA damage assays, analysis of complex samples, and real-time PCR detection
Product use One mL stains 100 minigels. Various pack sizes are available.
For Research Use Only. Not for use in diagnostic procedures.
Product Type Specs
Nucleic Acid Gel Stain
Label or Dye
SYBR™ Green I
Contents & Storage
Supplied as a 10,000X concentrate in DMSO. Store at -20°C, protected from light in a dessicator
Electrophoretic bandshift assay using SYBR® Green I Nucleic Acid Gel Stain.
DNA molecular weight ladders stained with SYBR® Green I Nucleic Acid Gel Stain.
HL-60 cells in a comet assay.
Comparison of single-stranded oligonucleotide detection using SYBR® Green I Nucleic Acid Gel Stain and ethidium bromide.