Citations & References (100)

Invitrogen™

Streptavidin, Texas Red™ conjugate

Catalog Number	Quantity
S872	1 mg

Catalog number S872

Price (USD)

309.00

Quantity:

Price (USD)

309.00

The streptavidin, Texas Red™ conjugate has excitation and emission maxima ∼596/612 and is widely used to detect biotinylated probes by fluorescence microscopy.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product TypeStreptavidin Conjugate (fluorescent)

Quantity1 mg

Shipping ConditionRoom Temperature

ConjugateTexas Red

FormSolid

Product LineTexas Red

Unit SizeEach

Contents & Storage

Store in freezer (-5 to -30°C) and protect from light.

Frequently asked questions (FAQs)

I am planning to use a fluorescent streptavidin labeled conjugate. What are the storage conditions and shelf life for the lyophilized powder and reconstituted solution?

In the lyophilized powder form, the fluorescent streptavidin labeled conjugate is stable for six months when stored at -20 degrees C, desiccated, and protected from light. The reconstituted solution is stable for approximately six months when stored at 4 degrees C, protected from light, with the addition of sodium azide to a final concentration of 5 mM or thimerosal to 0.2 mM. For longer storage, we recommend dividing the solution into aliquots and freezing at -20 degrees C, protected from light. Avoid repeated freezing and thawing of the solution.

I am planning to use a fluorescent streptavidin labeled conjugate. How should I prepare the working solution of the conjugate?

The fluorescent streptavidin labeled conjugate solution can be made by dissolving the powder in 0.5-1.0 mL of PBS or other suitable buffer. For details, please refer to page 4 of the "Streptavidin and Fluorescent Conjugates of Streptavidin" manual (https://assets.fishersci.com/TFS-Assets/LSG/manuals/mp00888.pdf).

Citations & References (100)

Citations & References

Abstract

The zinc finger protein A20 inhibits TNF-induced NF-kappaB-dependent gene expression by interfering with an RIP- or TRAF2-mediated transactivation signal and directly binds to a novel NF-kappaB-inhibiting protein ABIN.

Authors:Heyninck K,De Valck D,Vanden Berghe W,Van Criekinge W,Contreras R,Fiers W,Haegeman G,Beyaert R

Journal:The Journal of cell biology

PubMed ID:10385526

The zinc finger protein A20 is a tumor necrosis factor (TNF)â and interleukin 1 (IL-1)-inducible protein that negatively regulates nuclear factor-kappa B (NF-ÎºB)âdependent gene expression. However, the molecular mechanism by which A20 exerts this effect is still unclear. We show that A20 does not inhibit TNF- induced nuclear translocation and ... More

In situ polymerase chain reaction amplification of HIV-1 DNA in brain tissue.

Authors:Strappe PM, Wang TH, McKenzie CA, Lowrie S, Simmonds P, Bell JE

Journal:J Virol Methods

PubMed ID:9562406

A direct in situ polymerase chain reaction (IS-PCR) assay is described for the detection of HIV-1 proviral DNA in formalin fixed paraffin embedded brain tissue. Biotin-16-dUTP is incorporated during the PCR process and microwave pretreatment of tissue sections ensures that no non-specific incorporation into damaged or nicked genomic DNA occurs. ... More

Organization of long-range inhibitory connections with rat visual cortex.

Authors:McDonald CT, Burkhalter A

Journal:J Neurosci

PubMed ID:7678860

We have studied the laminar organization of local long-range inhibitory connections within rat primary visual cortex (area 17) by combining retrograde tracing of nerve cell bodies with glutamic acid decarboxylase immunocytochemistry. While most inhibitory connections are confined to within 0.4 mm of the injection site, a subset of neurons at ... More

Fibronectin is a component of the sodium dodecyl sulfate-insoluble transglutaminase substrate.

Authors:Tyrrell DJ, Sale WS, Slife CW

Journal:J Biol Chem

PubMed ID:2897368

Liver plasma membranes contain a morphologically distinct protein complex which serves as a substrate for the plasma membrane-associated transglutaminase. The complex, which appears as a two-dimensional sheet, is insoluble in sodium dodecyl sulfate and reducing agents and has been named SITS for sodium dodecyl sulfate-insoluble transglutaminase substrate (Tyrrell, D. J., ... More

Autoantigens targeted in systemic lupus erythematosus are clustered in two populations of surface structures on apoptotic keratinocytes.

Authors:Casciola-Rosen LA, Anhalt G, Rosen A

Journal:J Exp Med

PubMed ID:7511686

'Systemic lupus erythematosus is a multisystem autoimmune disease in which the autoantibody response targets a variety of autoantigens of diverse subcellular location. We show here that these autoantigens are clustered in two distinct populations of blebs at the surface of apoptotic cells. The population of smaller blebs contains fragmented endoplasmic ... More

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