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Super Bright Complete Staining Buffer, eBioscience, is designed for use as a supplement to Flow Cytometry Staining Buffer in immunofluorescent staining protocols of cells in suspension. Super Bright Complete Staining Buffer is only necessary when using more than one polymer dye-conjugated antibody in the same sample to prevent non-specific polymer interactions, which can result in data appearing under-compensated. Super Bright Complete Staining Buffer is provided in a convenient 5 µL/test format and is compatible with traditional fluorochromes, Brilliant Violet dyes, and standard flow cytometry protocols.
Super Bright Complete Staining Buffer is not compatible with UltraComp eBeads microspheres. If using UltraComp eBeads microspheres as a compensation tool, Flow Cytometry Staining Buffer should be used exclusively for all dilutions and washes during staining of the microspheres.
Super Bright Complete Staining Buffer is a fully compatible replacement for Super Bright Staining Buffer (Cat. Nos. SB-4400-42, SB-4400-75), and it shows decreased background in the 450/40 channel on the violet laser.
Super Bright polymer dyes are sold under license from Becton, Dickinson and Company.