phiX174 RF1 DNA
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phiX174 RF1 DNA
Thermo Scientific™

phiX174 RF1 DNA

Thermo Scientific phiX174 was the first DNA virus discovered to have a single-stranded, circular genome. phiX174 DNA is 5386 ntRead more
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Catalog NumberQuantity
SD003150 μg
Catalog number SD0031
Price (USD)/ 50 µg
170.00
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Quantity:
50 μg
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Price (USD)/ 50 µg
170.00
Add to cart
Thermo Scientific phiX174 was the first DNA virus discovered to have a single-stranded, circular genome. phiX174 DNA is 5386 nt in length. The DNA strand packaged into the virion is termed the “plus” strand. After entering the cell, the Thermo Scientific phiX174 DNA is used as a template for minus-strand synthesis, producing double-stranded DNA. The conversion of plus DNA strands to double-stranded DNA does not require any of the phage genes to function.

The double-stranded DNA can then be transcribed, resulting in synthesis of phage-encoded proteins. Synthesis of single-stranded (plus) DNA requires phage-encoded gene A protein. DNA synthesis is initiated at ori (+) and proceeds in the direction indicated. Late in infection, the single-stranded circles are encapsidated into new virions. The cycle terminates by cellular lysis, mediated by phage gene E encoded protein.

Highlights

• Purified by chromatography using proprietary patented technology
• More than 90% in the supercoiled form
• Isolated from E. coli HF4704 infected with phiX174 am3

Applications

• Preparation of DNA molecular weight standards

GenBank/EMBL Accession Numbers
V01128, J02482, M10348, M10379, M10714, M10749, M10750, M10866, M10867, M24859

phiX174 plasmid usage notes:

For DNA sequence, sequence analysis and map creation, see the REviewer free online tool
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration0.5 μg/μL
Final Product TypeDNA
For Use With (Application)Cloning/Nucleic Acid Gel Electrophoresis
Gel CompatibilityAgarose Gels
Product TypeDNA Reagent
Quantity50 μg
Sample TypeDNA
Unit Size50 µg