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Thermo Scientific™

Lambda DNA/EcoRI plus HindIII Marker

Green features
Catalog number: SM0191
Thermo Scientific™

Lambda DNA/EcoRI plus HindIII Marker

Green features
Catalog number: SM0191
Catalog Number
SM0191
Unit Size
5 x 50 µg
Price (USD)
Price: 98.00
Your Price:
Availability
***
Quantity
Catalog Number
Unit Size
25 x 50 µg
Price (USD)
Price: 366.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
SM01915 x 50 µg
Price: 98.00
Your Price:
***
SM019225 x 50 µg
Price: 366.00
Your Price:
***
Product Overview
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Additional Information
Recommendations
Recommendations
Thermo Scientific Lambda DNA/EcoRI+HindIII Marker is recommended for sizing of linear double-stranded large DNA fragments in agarose gels. Lambda DNA is digested to completion with the appropriate Thermo Scientific restriction enzyme(s) and purified and dissolved in storage buffer. DNA fragments contain various sticky ends depending on the restriction enzyme used for preparation of the marker.

Lambda DNA/EcoRI+HindIII Marker can be labeled radioactively with T4 Polynucleotide Kinase. Alternatively, and it can be labeled radioactively or non-radioactively with Klenow fragment or Klenow Fragment, exo- with the fill-in reaction (see Resources for Protocols).

Highlights

• Sizing and approximate quantification of large DNA fragments
• Sharp bands
• Supplied with loading dye for sample DNA

Note
The cohesive ends (the 12nt cos site of lambda DNA) of fragments indicated may anneal and form additional bands. These fragments can be separated by heating at 65°C for 5minutes and then cooling on ice for 3minutes.

The shortest fragments (oblique) are not visible in standard electrophoresis. Fragment lengths are predicted by computer analysis of respective DNA sequences.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Gel Type
Agarose
Product Type Specs
DNA/ECORI Plus Hind III Marker
Ready to Load
No
Gel Compatibility
Agarose Gels
Green Features
Sustainable packaging
Quantity
5 x 50 μg

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