NuPAGE™ Midi Tris-Acetate Welcome Pack, 3 to 8%

The NuPAGE Tris-Acetate Midi Welcome Pack provides all of the necessary NuPAGE gels, buffers, and reagents needed to begin using the SureLock Tandem Midi Gel Tank. The SureLock Tandem Midi Gel Tank provides for rapid electrophoresis of midi gels using minimal buffer (∼520 mL/gel) in a leak-free system. With a setup time of ∼30 seconds, the tank efficiently runs midi gels while also providing consistent performance. The tank features two independent chambers, allowing electrophoresis of one or two gels at a time, which saves on buffer and limits waste.

The NuPAGE Tris-Acetate Midi Welcome Pack contains:
• SureLock Tandem Midi Gel Tank (STM1001)
• NuPAGE Tris-Acetate Midi Gels (2 boxes, 20 gels)
• NuPAGE Tris-Acetate SDS Running Buffer, 20X (LA0041)
• NuPAGE LDS Sample Buffer, 4X (NP0007)
• NuPAGE Sample Reducing Agent, 10X (NP0009)
• HiMark Pre-stained Protein Standard (LC5699)

About the SureLock Tandem Midi Gel Tank
The SureLock Tandem Midi Gel Tank provides for rapid electrophoresis of midi gels using minimal buffer (∼520 mL/gel) in a leak-free system. With a setup time of ∼30 seconds, the tank efficiently runs midi gels while also providing consistent performance. The tank features two independent chambers, allowing electrophoresis of one or two gels at a time, which saves on buffer and limits waste. The tank is compatible with all Invitrogen pre-cast midi gels and midi gel cassettes.

About NuPAGE Tris-Acetate Midi Gels
NuPAGE Tris-Acetate Midi protein gels provide excellent separation of large molecular weight proteins. NuPAGE Tris-Acetate Midi gels are high performance polyacrylamide gels that simulate the denaturing or the native conditions of the traditional Laemmli system. The unique buffer formulation maintains a low operating pH during electrophoresis, resulting in superior resolution of proteins compared to traditional Tris-glycine midi SDS-PAGE gels.

Transfer and detection
For transfer of proteins to a membrane, we recommend using NuPAGE Transfer Buffer (NP0006) for traditional wet transfer using the SureLock Tandem Midi Blot Module (STM2001). Alternatively, rapid semi-dry transfer can be done using the Invitrogen Power Blotter or rapid dry transfer using the iBlot 2 Gel Transfer Device (IB21001).