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Invitrogen™
ThiolTracker™ Violet (glutathione detection reagent)
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Description
ThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority of intracellular free thiols in the cell, ThiolTracker™ Violet can be used in estimating the cellular level of reduced glutathione.ThiolTracker™ Violet offers the following;
• 10X brighter than mBCL
• UV or 405 nm excitation, 525 nm emission
• Easy to use
• Reproducible results
• Can be fixed with aldehydes and permeabilized by Triton® X-100 (0.5%)
• Formaldehyde fixable and suvives detergent extraction
• Can be used in multiplex assays including cytotoxicity studies
Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection. ThiolTracker™ Violet can be used for Flow Cytometry, HCS imaging, and epifluorescent microscopy.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| For Use With (Equipment): | Flow Cytometer, Fluorescence Microscope |
|---|---|
| Detection Method: | Fluorescent |
| Format: | Slide(s), Tube(s) |
| Color: | Violet |
| Emission Class: | Visible |
| Excitation Class: | UV, Visible |
| Excitation⁄Emission (nm): | 405⁄526 |
| Form: | Lyophilized |
| Label or Dye: | ThiolTracker™ Violet |
| Number of Reactions: | 180 Reactions |
| Product Line: | Molecular Probes®, ThiolTracker™ |
| Product Size: | 180 assays |
| Shipping Condition: | Room Temperature |
Contents & storage
Store at ≤–20°C, L protect material from long-term exposure to light; may be exposed to light for short periods of time, requires DMSO
