ThiolTracker™ Violet (glutathione detection reagent)
ThiolTracker™ Violet (glutathione detection reagent)
Invitrogen™

ThiolTracker™ Violet (glutathione detection reagent)

ThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents theRead more
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Catalog NumberQuantity
T10095180 reactions
Catalog number T10095
Price (USD)
582.00
180 assays
Add to cart
Quantity:
180 reactions
Price (USD)
582.00
180 assays
Add to cart
ThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority of intracellular free thiols in the cell, ThiolTracker™ Violet can be used in estimating the cellular level of reduced glutathione.
ThiolTracker™ Violet offers the following;
• 10X brighter than mBCL
• UV or 405 nm excitation, 525 nm emission
• Easy to use
• Reproducible results
• Can be fixed with aldehydes and permeabilized by Triton® X-100 (0.5%)
• Formaldehyde fixable and suvives detergent extraction
• Can be used in multiplex assays including cytotoxicity studies
Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection. ThiolTracker™ Violet can be used for Flow Cytometry, HCS imaging, and epifluorescent microscopy.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorViolet
Detection MethodFluorescence
Dye TypeThiolTracker™ Violet
EmissionVisible
Excitation Wavelength Range405⁄526
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
FormLyophilized
FormatTube(s), Slide(s)
Product LineMolecular Probes™, ThiolTracker™
Quantity180 reactions
Shipping ConditionRoom Temperature
Label TypeOther Labels or Dyes
Product TypeActin
Unit Size180 assays
Contents & Storage

Store at ≤–20°C, L protect material from long-term exposure to light; may be exposed to light for short periods of time, requires DMSO

Frequently asked questions (FAQs)

Is ThiolTracker Violet a fluorogenic reagent?

No. ThiolTracker Violet is fluorescent whether bound or unbound to a sulfhydryl group.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Will ThiolTracker Violet bind to proteins as well as glutathione?

Yes, ThiolTracker Violet will bind to any accessible sulfhydryl group (-SH; cysteine) whether it is on reduced glutathione or on the surface of a protein. It will not bind to sulfur bound via a disulfide bond (cystine) or ‘buried’ cysteines.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.