Get upto 200X more sensitivity in ICC/IHC/FISH for more clarity and definition with new Tyramide SuperBoost™ kits

ThiolTracker™ Violet (glutathione detection reagent)

Catalog number: T10096

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Flow Cytometry

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

ThiolTracker™ Violet is a brighter and more robust intracellular thiol probe for reproducible GSH detection. Since reduced glutathione represents the majority of intracellular free thiols in the cell, ThiolTracker™ Violet can be used in estimating the cellular level of reduced glutathione.
ThiolTracker™ Violet offers the following;
• 10X brighter than mBCL
• UV or 405 nm excitation, 525 nm emission
• Easy to use
• Reproducible results
• Can be fixed with aldehydes and permeabilized by Triton® X-100 (0.5%)
• Formaldehyde fixable and suvives detergent extraction
• Can be used in multiplex assays including cytotoxicity studies
Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection. ThiolTracker™ Violet can be used for Flow Cytometry, HCS imaging, and epifluorescent microscopy.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Detection Method: Fluorescent
Format: Slide(s), Tube(s), 96-well plate
Form: Lyophilized
Color: Violet
Label or Dye: ThiolTracker™ Violet
Product Size: 5 microplates
Emission Class: Visible
Excitation Class: Visible, UV
Excitation⁄Emission (nm): 405⁄526
Shipping Condition: Room Temperature

Contents & storage

Store at ≤–20°C, L protect material from long-term exposure to light; may be exposed to light for short periods of time, requires DMSO

Documents

Manuals & protocols

Recommended products