Invitrogen™

Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Catalog number: T1014
Invitrogen™

Galacto-Star™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Catalog number: T1014
Catalog Number
T1014
Unit Size
1,800 assays
Price (USD)
Price: 900.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
600 assays
Price (USD)
Price: 421.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
T10141,800 assays
Price: 900.00
Your price:
-
T1012600 assays
Price: 421.00
Your price:
-
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The Galacto-Star™ One-Step β-galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of β-galactosidase in mammalian cell lysates.

• Glow assay kinetics provides a “window” during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems.
• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Sensitive
The chemiluminescent assay exhibits over three orders of magnitude greater sensitivity than colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.

Versatile
The Galacto-Star™ system includes Galacton-Star™ chemiluminescent substrate for β-galactosidase and is suited for measurement in both luminometers and scintillation counters. Automatic injection is not required.

Long-Lasting Signal
Light emission from the Galacto-Star™ assay reaches maximum in 60-90 minutes and remains constant for nearly one hour. Cell lysate is mixed with reaction buffer containing Galacton-Star™ substrate and Sapphire-II™ luminescence enhancer. After a 60-90 minute incubation at room temperature, the signal is measured in a luminometer or scintillation counter.

For Research Use Only. Not for use in diagnostics procedures.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
β-Galactosidase Reporter Gene Assay System
Detection Method
Chemiluminescent
For Use With (Equipment)
Luminometer (Microplate)
Assay
β-Gal (lacZ) Assay
Compatible Cells
Mammalian Cells
High-throughput Compatibility
High-throughput Compatible
Label or Dye
Unconjugated
Readout
End Point
Sample Type
Cell Cultures
Substrate
Galacton-Star™
Substrate Type
Beta-Gal Substrate
Substrate Properties
Chemical Substrate
Target Enzyme
Beta-Galactosidase
Product Line
Galacto-Star™, NovaBright™
For Use With (Application)
Enzyme Assay
Quantity
1800 Assays
Form
Liquid

Contents & Storage

This kit contains the lysis solution, substrate, enhancer, and required buffer. Store the product at 4°C.

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