Invitrogen™

Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Catalog number: T1029
Invitrogen™

Gal-Screen™ β-Galactosidase Reporter Gene Assay System for Mammalian Cells

Catalog number: T1029
Catalog Number
T1029
Unit Size
200 assays
Price (USD)
Price: 345.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
1,000 assays
Price (USD)
Price: 1,008.00
Your price:
Availability
-
Quantity
Catalog Number
Unit Size
10,000 assays
Price (USD)
Price: 6,370.00
Your price:
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
T1029200 assays
Price: 345.00
Your price:
-
T10271,000 assays
Price: 1,008.00
Your price:
-
T102810,000 assays
Price: 6,370.00
Your price:
-
Product Overview
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Additional Information
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The Gal-Screen™ assay system combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

• Homogeneous assay format allows detection of β-galactosidase in the presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible.
• The kinetics of the glow-assay provides a “window” during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Can be used with either mammalian or yeast model systems, providing flexibility in choice of model systems.
• Wide dynamic range of β-galactosidase assay enables accurate measurement of enzyme from femtogram to nanogram range.
• Assay sensitivity is 100 to 1,000-fold better than either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assay technologies.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Ideal for Screening
This homogeneous assay is ideally suited for screening applications where assay automation is required. The Gal-Screen™ system uses Galacton-Star™ chemiluminescent substrate for convenient measurement in a luminometer. Light emission reaches maximum in 60-90 minutes and remains constant for 45-90 minutes.

For Research Use Only. Not for use in diagnostics procedures.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Product Type
β-Galactosidase Reporter Gene Assay System
Detection Method
Chemiluminescent
For Use With (Equipment)
Luminometer (Microplate)
Assay
β-Gal (lacZ) Assay
Compatible Cells
Mammalian Cells
High-throughput Compatibility
High-throughput Compatible
Label or Dye
Unconjugated
Readout
End Point
Sample Type
Cell Cultures
Substrate
Galacton-Star™
Substrate Type
Beta-Gal Substrate
Substrate Properties
Chemical Substrate
Target Enzyme
Beta-Galactosidase
Product Line
Gal-Screen™, NovaBright™
For Use With (Application)
Enzyme Assay
Quantity
200 Assays
Form
Liquid

Contents & Storage

This kit contains the enzyme substrate and the reaction buffer.
Store the product at 4°C.

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