Luc-Screen™ Extended-Glow Luciferase Reporter Gene Assay System

Description

The Luc-Screen® assay system with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme in microplate cell cultures. A linear signal is obtained with the Luc-Screen® assay from 50 fg to 100 ng of pure enzyme in culture medium.

• Homogeneous assay format allows detection of firefly luciferase in the presence of normal culture media without removal of media and without an additional cell lysis step, providing the easiest and most streamlined assay procedure possible.
• The glow assay kinetics provides a "window" during which measurements may be performed, facilitating HTS applications where assay automation is used.
• Wide dynamic range of firefly luciferase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than with either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for b-galactosidase, providing greater sensitivity than competing assay technologies.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Sensitive
Luciferase is an ideal reporter because of the high sensitivity of detection and the absence of endogenous luciferase activity in mammalian cells. The Luc-Screen® assay conveniently couples in-well cell lysis in the presence of culture medium with a high sensitivity assay that exhibits extended-glow light emission kinetics. Light signal can be measured between 10 minutes and several hours after adding assay reagents, providing great flexibility in the time between reagent addition and measurement.

High Throughput
The Luc-Screen® system is designed for maximum assay flexibility in a high throughput format and can be used in luminometers without automatic injectors. The Luc-Screen® system is formulated to provide a convenient, easy-to-use luciferase assay that is optimized for use in high-throughput screening. The high sensitivity of the Luc-Screen® assay is accompanied by a wide dynamic range.

For Research Use Only. Not for use in diagnostics procedures.

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Specifications

Assay Type:	Luciferase Assay
Compatible Cells:	Mammalian Cells
Detection Method:	Chemiluminescent
For Use With (Equipment):	Luminometer (Microplate)
Form:	Liquid
Format:	96-well plate
High Throughput Compatibility:	High Throughput-Compatible
Product Line:	Luc-Screen®, NovaBright™
Product Size:	200 assays
Readout:	End Point
Sample Type (Specific):	Cell Cultures
Substrate:	Luciferin
Substrate Properties:	Chemical Substrate
Substrate Type:	Luciferase Substrate
Target Enzyme:	Luciferase
Technique:	Enhanced Chemiluminescence

Contents & storage