Search
Citas y referencias (16)
Invitrogen™
Kit de tamaño de microesferas fluorescentes TetraSpeck™ (montado sobre portaobjetos)
Este kit de tamaños de microesferas fluorescentes TetraSpeck contiene un portaobjetos para microscopio, con seis regiones de visualización. Cada unaMás información
| Número de catálogo | Cantidad |
|---|---|
| T14792 | 1 kit |
Número de catálogo T14792
Precio (MXN)
11,672.19
1 kit
Cantidad:
1 kit
Precio (MXN)
11,672.19
1 kit
Este kit de tamaños de microesferas fluorescentes TetraSpeck contiene un portaobjetos para microscopio, con seis regiones de visualización. Cada una de las cinco regiones de visualización contiene microesferas de un tamaño: de 0,1, 0,2, 0,5, 1,0 o 4,0 µm. La región restante contiene una mezcla de los cinco tamaños de gránulos. Cada microesfera se tiñe con cuatro colorantes fluorescentes diferentes [365⁄430 nm (azul), 505⁄515 nm (verde), 560⁄580 nm (naranja) y 660⁄680 nm (rojo oscuro)] que tienen picos de excitación y emisión claramente separados. Las microesferas fluorescentes de Invitrogen TetraSpeck™ ayudan en la calibración de microscopios de campo amplio, TIRF y fluorescencia confocal, especialmente para aplicaciones multicolores como el análisis de colocalización y la derivación de funciones de dispersión de puntos críticas para la deconvolución.
Consulte nuestra completa colección de reactivos de calibración para microscopio ›
Consulte nuestra completa colección de reactivos de calibración para microscopio ›
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de calibraciónCalibración de microscopio confocal, calibración de microscopio de fluorescencia
FormatoMontado en portaobjetos
Línea de productosTetraSpeck
Cantidad1 kit
Condiciones de envíoTemperatura ambiente
ColorNaranja, rojo oscuro, azul, verde, Dark Red, Blue, Green
Diámetro (métrico)0,5 μm, 0,2 μm, 0,1 μm, 4 μm, 1 μm
Tipo de productoKit de tamaños de microesferas fluorescentes
Unit Size1 kit
Contenido y almacenamiento
Almacenar a temperatura ambiente y proteger de la luz.
Preguntas frecuentes
What are the excitation/emission peaks for TetraSpeck Microspheres?
The TetraSpeck Microspheres (Cat. Nos. T7279, T7280, T7281, T7283, T7284, T14792) are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks at 360/430 nm (blue), 505/515 nm (green), 560/580 nm (orange) and 660/680 nm (dark red).
TetraSpeck Blue Dye Spectra
Fluorescence excitation and emission spectra of bead encapsulated TetraSpeck blue dye.
TetraSpeck Orange Dye Spectra
TetraSpeck Green Dye Spectra
TetraSpeck Dark Red Dye Spectra
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Citations & References (16)
Citations & References
Abstract
The cohesion protein ORD is required for homologue bias during meiotic recombination.
Journal:J Cell Biol
PubMed ID:15007062
'During meiosis, sister chromatid cohesion is required for normal levels of homologous recombination, although how cohesion regulates exchange is not understood. Null mutations in orientation disruptor (ord) ablate arm and centromeric cohesion during Drosophila meiosis and severely reduce homologous crossovers in mutant oocytes. We show that ORD protein localizes along
Exocytosis of IgG as mediated by the receptor, FcRn: an analysis at the single-molecule level.
Journal:Proc Natl Acad Sci U S A
PubMed ID:15258288
'IgG transport within and across cells is essential for effective humoral immunity. Through a combination of biochemical and in vivo analyses, the MHC class I-related neonatal Fc receptor (FcRn) is known to play a central role in delivering IgGs within and across cells. However, little is known about the molecular
Colocalization of fluorescent markers in confocal microscope images of plant cells.
Journal:Nat Protoc
PubMed ID:18388944
'This protocol describes the steps needed to perform quantitative statistical colocalization on two-color confocal images, specifically of plant cells. The procedure includes a calibration test to check the chromatic alignment of the confocal microscope. A software tool is provided to calculate the Pearson and Spearman correlation coefficients (''Pearson-Spearman correlation colocalization''
Mast cell degranulation requires N-ethylmaleimide-sensitive factor-mediated SNARE disassembly.
Journal:J Immunol
PubMed ID:14607937
'Mast cells possess specialized granules that, upon stimulation of surface FcR with IgE, fuse with the plasma membrane, thereby releasing inflammatory mediators. A family of membrane fusion proteins called SNAREs, which are present on both the granule and the plasma membrane, plays a role in the fusion of these granules
Three-dimensional random access multiphoton microscopy for functional imaging of neuronal activity.
Journal:Nat Neurosci
PubMed ID:18432198
The dynamic ability of neuronal dendrites to shape and integrate synaptic responses is the hallmark of information processing in the brain. Effectively studying this phenomenon requires concurrent measurements at multiple sites on live neurons. Substantial progress has been made by optical imaging systems that combine confocal and multiphoton microscopy with