This TetraSpeck Fluorescent Microspheres Size Kit contains one microscope slide, with six viewing regions. Each of five viewing regions contains microspheres of one size – 0.1, 0.2, 0.5, 1.0 or 4.0 µm. The remaining region contains a mixture of all five bead sizes. Every microsphere is stained with four different fluorescent dyes [365⁄430 nm (blue), 505⁄515 nm (green), 560⁄580 nm (orange), and 660⁄680 nm (dark red)] that have well-separated excitation and emission peaks. Invitrogen’s TetraSpeck™ fluorescent microspheres aid in the calibration of wide field, TIRF and confocal fluorescence microscopes,especially for multicolor applications like co-localization analysis and the derivation of point spread functions critical for deconvolution.

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