Invitrogen™

I-Block™ Protein-Based Blocking Reagent

Catalog number: T2015
Invitrogen™

I-Block™ Protein-Based Blocking Reagent

Catalog number: T2015
Catalog Number
T2015
Unit Size
30 g
Price (USD)
72.25
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
T201530 g
72.25
Your Price:
***
Product Overview
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I-Block reagent is a highly purified casein-based blocking reagent. It provides superior blocking compared to both dried milk and BSA. Unlike other casein-based blocking reagents, I-Block reagent is essentially biotin-free. I-Block reagent is tested in assays using Tropix substrates and alkaline phosphatase conjugates, and is useful as a blocking reagent in membrane-based and immunoassay applications.

The suggested working concentration is 0.2% (w/v) for detection of nucleic acids on neutral or positively-charged nylon membranes and for immunoassays and protein detection on membranes (nitrocellulose, PVDF, or neutral nylon). For protein detection on positively-charged nylon membrane (such as TropilonPlus membrane), a concentration of 3% is recommended. I-Block blocking solution is prepared in either Tris- or phosphate-buffered saline buffer with heating (40-50°C).

This casein-based blocking reagent can be used with Western-Light, Western-Light Plus, Western-Star, ELISA-Light, Southern-Light, and Southern-Star detection systems.

Specifications

Buffer
Blocking Buffers
Conjugate
Alkaline Phosphatase
Product Line
I-Block™, NovaBright™
Product Type
Reagent
Quantity
30 g
Substrate
Tropix
For Use With (Equipment)
Western-Light™ Detection System, Western-Star™ Detection System, Southern-Star™ Detection System, ELISA-Light™ Detection System, Southern-Light™ Detection System, Western-Light Plus™ Detection System
Shipping Condition
Room Temperature

Contents & Storage

Store the product at room temperature.

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