Nitro-Block™ Enhancer

Catalog number: T2026

Applied Biosystems™  Related applications: Protein Assays and Analysis

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Nitro-Block Enhancer can be used with CSPD chemiluminescent substrate to increase the alkaline phosphatase signal for protein or nucleic acid blots. Nitrocellulose membranes provide an inefficient environment for chemiluminescence, resulting in very low signal intensity. Tropix Nitro-Block and Nitro-Block-II membrane enhancers increase signal intensity on both nitrocellulose and PVDF membranes.

Nitro-Block and Nitro-Block-II enhancers generate a hydrophobic environment on the membrane surface that increases the intensity of chemiluminescence. Without Nitro-Block Enhancer, the signal on nitrocellulose membranes is weak, making detection either impossible or requiring extremely long exposure times. With Nitro-Block Enhancer, short exposures of 10 to 45 minutes are achieved. While PVDF membranes do not require the use of Nitro-Block Enhancer, exposure times are ten-fold faster with Nitro-Block Enhancer. This permits very short exposure times on PVDF, ranging from 15 seconds to 15 minutes for western blots.

For Research Use Only. Not for use in diagnostic procedures.


Form: Liquid
Format: Bottle(s)
Product Line: Nitro-Block™, NovaBright™
Product Size: 20 mL
Substrate: Alkaline Phosphatase
Substrate Type: AP (Alkaline Phosphatase) Substrate
Shipping Condition: Room Temperature

Contents & storage

Store the product at 4°C.


Manuals & protocols