Luminescence Enhancer For Solution Assays, Emerald-II™ Enhancer

Catalog number: T2115

Invitrogen™  Related applications: Protein Assays and Analysis

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The Sapphire-II™ chemiluminescence enhancer significantly reduces aqueous quenching and tune the wavelength of light emission.

• Aqueous environments reduce chemiluminescent signal intensity of 1,2-dioxetane substrates by water-induced quenching; chemiluminescence enhancers increase the emission efficiency of light production by partitioning the water from the site of chemiluminescent signal production.
• Chemically optimized formulations enhance signal intensity in solution-based applications with dioxetane-based substrate solutions (CSPD®, CDP-Star®, Galacton®, Galacton-Plus®, Galacton-Star®, or NA-Star® substrates).
• Choice of Sapphire-II™ enhancer facilitates assay development by allowing users to optimize assay signal intensity and wavelength of light emission.

Optimize Chemiluminescence Signals
Sapphire-II™ chemiluminescence enhancers, are essential components of tube or microplate-based assays, significantly reducing the effect of aqueous quenching. Enhancers also tune the wavelength of light emission. Sapphire-II™ enhancer shifts the light emission maximum to 461 nm from 475 nm observed with the dioxetane alone. The use of Sapphire-II™ enhancer can produce a wide dynamic range since photodetector saturation is less likely to occur at higher sample concentrations.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.


Form: Liquid
Format: Bottle(s)
Product Line: EMERALD-II™, NovaBright™
Product Size: 2500 assays
Substrate: Alkaline Phosphatase
Substrate Type: AP (Alkaline Phosphatase) Substrate
Shipping Condition: Room Temperature

Contents & storage

Store the product at 4°C.


Manuals & protocols